Mercury ion separation from another metal ions has been carried out in phase system consisted of propanol, NaCl and KI. The results showed that propanol could extract the associates formed by mercury with iodide. Hg(...Mercury ion separation from another metal ions has been carried out in phase system consisted of propanol, NaCl and KI. The results showed that propanol could extract the associates formed by mercury with iodide. Hg(Ⅱ) can be separated completely from Fe(Ⅲ), Co(Ⅱ), Mn(Ⅱ), Mo(Ⅵ) and Zn(Ⅱ) in solution system of pH=2.0.展开更多
OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to ...OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress.METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the procedure.In vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods.RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP treatment.The abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP treatment.More apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell injury.CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury.展开更多
Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal i...Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal imaging system was selected to measure the infrared radiation temperature on body surface of eight points in four meridians in 74 patients with hyperplasia of mammary glands and 63 healthy people. Results： The infrared radiation temperatures on body surface of right Youmen （KI 21） in cases with hyperplasia of mammary glands were significantly higher than the healthy people （P=-0.009）, and the infrared radiation temperatures on left Youmen （KI 21） and other points had no significant difference with healthy people （P〉0.05）. Conclusion： The infrared radiation temperatures on body surface of right Youmen （KI 21） have significant differences with healthy people. This might occur because of the pathogenesis of hyperplasia of mammary glands and of the specificity of the point Youmen （KI 21）.展开更多
AIM To evaluate the relationship between the expression of Ki 67 antigen and the pathobiological behaviours of gastric cancers especially their distant metastases. METHODS Fifty six specimens of gastric cancer ro...AIM To evaluate the relationship between the expression of Ki 67 antigen and the pathobiological behaviours of gastric cancers especially their distant metastases. METHODS Fifty six specimens of gastric cancer routinely fixed in formalin and embedded in paraffin (FFEP) were studied by immunohistochemical method. RESULTS Expression of Ki 67 antigen was significantly related to the distant metastases to liver, ovary and adrenal gland ( P <0 005), but not related to the histological type, growth pattern, depth of invasion, histological differentiation and the metastases to local lymph nodes ( P >0 05). Furthermore, the Ki 67 antigen expression was significantly related to the DNA aneuploidy pattern, which is closely related to poor prognosis ( P <0 05). CONCLUSION Overexpression of Ki 67 can be used as an objective marker of the proliferative activity for predicting prognosis of gastric cancer and metastatic potential to distant organs.展开更多
OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in ...OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.METHODS:Fifty-two PD patients and 49 healthy volunteers were recruited.Skin temperature measurements were performed with a skin temperature assessment device at 10 points.Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.RESULTS:On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi(KI 3) compared with the healthy group(P < 0.01).A significant reduction in skin temperature difference was detected at Taixi(KI 3) in the first day of menstruation compared with those values in the third day after menstruation(P < 0.01)in the healthy group.On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu(LR 6) in PD group compared with the healthy group(P < 0.05).No significant differences of skin temperature were detected at other points(P > 0.05).CONCLUSION:The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD.Significant difference was only found in Taixi(KI 3), the Yuan-source point of Kidney meridian.展开更多
文摘Mercury ion separation from another metal ions has been carried out in phase system consisted of propanol, NaCl and KI. The results showed that propanol could extract the associates formed by mercury with iodide. Hg(Ⅱ) can be separated completely from Fe(Ⅲ), Co(Ⅱ), Mn(Ⅱ), Mo(Ⅵ) and Zn(Ⅱ) in solution system of pH=2.0.
基金The project supported by National Natural Science Foundation of China(81330074,81261160507,81170632,81370783,41376166)the 111Project,and International Science&Technology Cooperation Program of China 2012DFA11070
文摘OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress.METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the procedure.In vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods.RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP treatment.The abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP treatment.More apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell injury.CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury.
基金State 973 plan (2005CB523306)National Natural Science Foundation (30572306)+1 种基金Shanghai Municipal Education Commission Project (07CZ034)State Administration of Traditional Chinese Medicine and Shanghai Leading Academic Discipline Project (T0304,T0302)
文摘Objective： To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method： The thermaCATMT P30 infrared thermal imaging system was selected to measure the infrared radiation temperature on body surface of eight points in four meridians in 74 patients with hyperplasia of mammary glands and 63 healthy people. Results： The infrared radiation temperatures on body surface of right Youmen （KI 21） in cases with hyperplasia of mammary glands were significantly higher than the healthy people （P=-0.009）, and the infrared radiation temperatures on left Youmen （KI 21） and other points had no significant difference with healthy people （P〉0.05）. Conclusion： The infrared radiation temperatures on body surface of right Youmen （KI 21） have significant differences with healthy people. This might occur because of the pathogenesis of hyperplasia of mammary glands and of the specificity of the point Youmen （KI 21）.
文摘AIM To evaluate the relationship between the expression of Ki 67 antigen and the pathobiological behaviours of gastric cancers especially their distant metastases. METHODS Fifty six specimens of gastric cancer routinely fixed in formalin and embedded in paraffin (FFEP) were studied by immunohistochemical method. RESULTS Expression of Ki 67 antigen was significantly related to the distant metastases to liver, ovary and adrenal gland ( P <0 005), but not related to the histological type, growth pattern, depth of invasion, histological differentiation and the metastases to local lymph nodes ( P >0 05). Furthermore, the Ki 67 antigen expression was significantly related to the DNA aneuploidy pattern, which is closely related to poor prognosis ( P <0 05). CONCLUSION Overexpression of Ki 67 can be used as an objective marker of the proliferative activity for predicting prognosis of gastric cancer and metastatic potential to distant organs.
基金Supported by the National Basic Research Program of China(973 Program)-the Effect of De Qi on Acupoint Specific Effect Based on Meridians and its Characteristics and Molecular Response Mechanisms(No.2012CB518506)Research on Acupoint Specificity in Regulating Uterus(No.2006CB504503)National Natural Science Foundation of China-Research on Variations of Biophysical Properties of Acupoints on Different Meridians with the Same Spinal Segments Based on Primary Dysmenorrhea(No.81573884)
文摘OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.METHODS:Fifty-two PD patients and 49 healthy volunteers were recruited.Skin temperature measurements were performed with a skin temperature assessment device at 10 points.Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.RESULTS:On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi(KI 3) compared with the healthy group(P < 0.01).A significant reduction in skin temperature difference was detected at Taixi(KI 3) in the first day of menstruation compared with those values in the third day after menstruation(P < 0.01)in the healthy group.On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu(LR 6) in PD group compared with the healthy group(P < 0.05).No significant differences of skin temperature were detected at other points(P > 0.05).CONCLUSION:The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD.Significant difference was only found in Taixi(KI 3), the Yuan-source point of Kidney meridian.
