A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×...A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×10 -3 mol/L), HSA may combine with 3- by intermolecular forces (mainly by electrostatic force) to form { 3- n-HSA m+} k nanoparticle of the ion-association complexes of HSA m+- 3- n. There is a strongest resonance scattering intensity at 351 nm, owing to the existence of the resonance scattering of the nanoparticle, 3- molecular absorption and the non-distribution of the emission intensity of Xe lamp in the range of 200-1000 nm. In addition, two resonance scattering peaks at 470 and 700 nm were observed. The HSA concentration in the range of 0-12 μg/mL is linear to the resonance scattering intensity at 351 nm. The determination limit of this method is 0.1 μg/mL HSA, about ten-fold lower than that of Coomassie brilliant blue protein assay. This method has been used for the determination of HSA in human serum and synthetic samples with satisfactory results. The mechanism of enhanced resonance scattering light, the TEM of the particle, the concepts of quasi-elastic absorption and un-elastic absorption were also discussed.展开更多
An 8-week feeding trial investigated the optimum dietary vitamin K3 requirements of largemouth bass.A total of 600 healthy fish(12.96±0.07 g)were fed diets containing varying levels of vitamin K3 at 0.78(K0),5.80...An 8-week feeding trial investigated the optimum dietary vitamin K3 requirements of largemouth bass.A total of 600 healthy fish(12.96±0.07 g)were fed diets containing varying levels of vitamin K3 at 0.78(K0),5.80(K5),10.82(K10),15.84(K15),and 20.85(K20)mg/kg with four replicates per level.Results showed that dietary vitamin K3 enhanced growth,with weight gain rate and specific growth rate in K15 trials significantly higher than K0 trials(P<0.05).No significant difference was observed in the content of crude protein,crude lipid,moisture,ash,and phosphorus in muscle tissue(P>0.05).However,the supplementation of vitamin K3 significantly contributed to calcium accumulation in muscle tissues and vertebrae(P<0.05)and increased red blood cell counts(P<0.05).The blood coagulation time decreased significantly with increasing dietary vitamin K3 from 0.78 to 10.82 mg/kg(P<0.05)before stabilizing(P>0.05).The antioxidant capacity was improved with the addition of vitamin K3,which was inconsistent with the changes in malondialdehyde and MK-4(a form of menaquinone)within liver and serum,suggesting that the toxicity of excessive artificial vitamin K3 may account for this difference.Using regression analysis,the appropriate dietary vitamin K3 levels within a largemouth bass diet was determined to be in the range of 9.93-15.22 mg/kg,which will provide a reference for the preparation of vitamin premix and artificial diet for largemouth bass.展开更多
We study the space of stability conditions on K3 surfaces from the perspective of mirror symmetry. This is done in the attractor backgrounds(moduli). We find certain highly non-generic behaviors of marginal stability ...We study the space of stability conditions on K3 surfaces from the perspective of mirror symmetry. This is done in the attractor backgrounds(moduli). We find certain highly non-generic behaviors of marginal stability walls(a key notion in the study of wall crossings)in the space of stability conditions. These correspond via mirror symmetry to some nongeneric behaviors of special Lagrangians in an attractor background. The main results can be understood as a mirror correspondence in a synthesis of the homological mirror conjecture and SYZ mirror conjecture.展开更多
The quotient space of a K3 surface by a finite group is an Enriques surface or a rational surface if it is smooth.Finite groups where the quotient space are Enriques surfaces are known.In this paper,by analyzing effec...The quotient space of a K3 surface by a finite group is an Enriques surface or a rational surface if it is smooth.Finite groups where the quotient space are Enriques surfaces are known.In this paper,by analyzing effective divisors on smooth rational surfaces,the author will study finite groups which act faithfully on K3 surfaces such that the quotient space are smooth.In particular,he will completely determine effective divisors on Hirzebruch surfaces such that there is a finite Abelian cover from a K3 surface to a Hirzebrunch surface such that the branch divisor is that effective divisor.Furthermore,he will decide the Galois group and give the way to construct that Abelian cover from an effective divisor on a Hirzebruch surface.Subsequently,he studies the same theme for Enriques surfaces.展开更多
A simple and sensitive spectroelectrochemistry method for the determination of vitamin K3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K3 was reduced at a glassy carbo...A simple and sensitive spectroelectrochemistry method for the determination of vitamin K3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K3 was reduced at a glassy carbon electrode, and its product with characteristic fluorescence at 420 nm was determined with excitation wavelength at 309 nm. Under optimized electrochemical reaction conditions and fluorescent experiment parameters, the fluorescence intensity was proportional to the concentration of vitamin K3 in a range from 3.50×10^-7 to 1.05×10^-5 mol/L with a correlation coefficient of 0.9991, and detection limit was estimated to be 7.50× 10^-8 mol/L at a signal/noise ra- tio of 3. The relative standard deviation was less than 4.3%（n=5） and the recovery was in a range of 97%-105% for the determination of vitamin K3 in pharmaceutical preparations. The result is satisfactory for the determination of vitamin K3 as comparison to that from HPLC method.展开更多
文摘A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×10 -3 mol/L), HSA may combine with 3- by intermolecular forces (mainly by electrostatic force) to form { 3- n-HSA m+} k nanoparticle of the ion-association complexes of HSA m+- 3- n. There is a strongest resonance scattering intensity at 351 nm, owing to the existence of the resonance scattering of the nanoparticle, 3- molecular absorption and the non-distribution of the emission intensity of Xe lamp in the range of 200-1000 nm. In addition, two resonance scattering peaks at 470 and 700 nm were observed. The HSA concentration in the range of 0-12 μg/mL is linear to the resonance scattering intensity at 351 nm. The determination limit of this method is 0.1 μg/mL HSA, about ten-fold lower than that of Coomassie brilliant blue protein assay. This method has been used for the determination of HSA in human serum and synthetic samples with satisfactory results. The mechanism of enhanced resonance scattering light, the TEM of the particle, the concepts of quasi-elastic absorption and un-elastic absorption were also discussed.
基金study was supported by Guangdong province key field R&D program project(No.2020B0202010001)Shanghai collaborative innovation for aquatic animal genetics and breeding.
文摘An 8-week feeding trial investigated the optimum dietary vitamin K3 requirements of largemouth bass.A total of 600 healthy fish(12.96±0.07 g)were fed diets containing varying levels of vitamin K3 at 0.78(K0),5.80(K5),10.82(K10),15.84(K15),and 20.85(K20)mg/kg with four replicates per level.Results showed that dietary vitamin K3 enhanced growth,with weight gain rate and specific growth rate in K15 trials significantly higher than K0 trials(P<0.05).No significant difference was observed in the content of crude protein,crude lipid,moisture,ash,and phosphorus in muscle tissue(P>0.05).However,the supplementation of vitamin K3 significantly contributed to calcium accumulation in muscle tissues and vertebrae(P<0.05)and increased red blood cell counts(P<0.05).The blood coagulation time decreased significantly with increasing dietary vitamin K3 from 0.78 to 10.82 mg/kg(P<0.05)before stabilizing(P>0.05).The antioxidant capacity was improved with the addition of vitamin K3,which was inconsistent with the changes in malondialdehyde and MK-4(a form of menaquinone)within liver and serum,suggesting that the toxicity of excessive artificial vitamin K3 may account for this difference.Using regression analysis,the appropriate dietary vitamin K3 levels within a largemouth bass diet was determined to be in the range of 9.93-15.22 mg/kg,which will provide a reference for the preparation of vitamin premix and artificial diet for largemouth bass.
文摘We study the space of stability conditions on K3 surfaces from the perspective of mirror symmetry. This is done in the attractor backgrounds(moduli). We find certain highly non-generic behaviors of marginal stability walls(a key notion in the study of wall crossings)in the space of stability conditions. These correspond via mirror symmetry to some nongeneric behaviors of special Lagrangians in an attractor background. The main results can be understood as a mirror correspondence in a synthesis of the homological mirror conjecture and SYZ mirror conjecture.
文摘The quotient space of a K3 surface by a finite group is an Enriques surface or a rational surface if it is smooth.Finite groups where the quotient space are Enriques surfaces are known.In this paper,by analyzing effective divisors on smooth rational surfaces,the author will study finite groups which act faithfully on K3 surfaces such that the quotient space are smooth.In particular,he will completely determine effective divisors on Hirzebruch surfaces such that there is a finite Abelian cover from a K3 surface to a Hirzebrunch surface such that the branch divisor is that effective divisor.Furthermore,he will decide the Galois group and give the way to construct that Abelian cover from an effective divisor on a Hirzebruch surface.Subsequently,he studies the same theme for Enriques surfaces.
基金Supported by the National Nature Science Foundation of China(No.20635020)the Open-end Fund of State Key Labo-ratory of Fine Chemicals, China(No.KF0508)
文摘A simple and sensitive spectroelectrochemistry method for the determination of vitamin K3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K3 was reduced at a glassy carbon electrode, and its product with characteristic fluorescence at 420 nm was determined with excitation wavelength at 309 nm. Under optimized electrochemical reaction conditions and fluorescent experiment parameters, the fluorescence intensity was proportional to the concentration of vitamin K3 in a range from 3.50×10^-7 to 1.05×10^-5 mol/L with a correlation coefficient of 0.9991, and detection limit was estimated to be 7.50× 10^-8 mol/L at a signal/noise ra- tio of 3. The relative standard deviation was less than 4.3%（n=5） and the recovery was in a range of 97%-105% for the determination of vitamin K3 in pharmaceutical preparations. The result is satisfactory for the determination of vitamin K3 as comparison to that from HPLC method.
