Background Sepsis is a complex pathophysiologic process, which is the leading cause of death in the world. Many studies have revealed that Chinese medicine has shown tremendous advantages on the treatment of sepsis. O...Background Sepsis is a complex pathophysiologic process, which is the leading cause of death in the world. Many studies have revealed that Chinese medicine has shown tremendous advantages on the treatment of sepsis. Our study aims to explore the effect of Huanglian jiedu decoction and Jiawei huanglian jiedu decoction on organ function in septic rats. Methods SD rats were randomly divided into 7 groups, sham-group, cecal ligation and puncture group （CLP, 18h/72h）, Jiawei huanglian jiedu decoction group （JW, 18h/72h）, Huanglian jiedu decoc-tion group （W, 18h/72h）. Except the Sham group, the rats in other groups received cecal ligation and puncture method to induce sepsis. CLP groups were intragastric administration with normal saline after CLP. Huanglian jiedu decoction and Jiawei huanglian jiedu decoction groups were intragastric administration with Huanglian jie-du decoction and Jiawei huanglian jiedu decoction in the same time points. Glutamic-pyruvic transaminase （ALT）, glutamic oxalacetic transaminase （AST）, total bilirubin （TBIL）, creatinine（Cr）, urea, creatine kinase-MB （CK-MB）, lactate dehydrogenase （LDH） were detected by biochemical analyzer. Brain natriuretic peptide （BNP）, surfactant protein A （SP-A）, chemotactic factor for monocyte-1 （MCP-1）, D-lactic acid （D-LAC）, complement 5a, mobility group box-1 protein B 1 （HMGB-1）, interleukin 6 （IL-6） were analyzed by enzyme-linked immunosor-bent assay （ELISA）. Total lung capacity （TLC） and function residual capacity （FRC） were measured by buxco re-search system. The wet to dry weight ratio （W/D） was tested. Moreover, changes in tissue pathology were evaluat-ed using hematoxylin and eosin （H＆E） staining and electron microscope. Results The level of AST/ALT in se-rum was decreased after treatment with Huanglian jiedu decoction and Jiawei huanglian jiedu decoction. More-over, the level of TLC and FRC was significantly increased, and the level of SP-A, W/D was significantly de-creased展开更多
目的:研究早期舌癌局灶性切除术患者给予加味黄连解毒汤治疗后的临床疗效和复发率。方法:将80例早期舌癌局灶性切除术患者随机分为两组。对照组40例,给予常规治疗;研究组40例,给予常规治疗及加味黄连解毒汤治疗。比较两组患者生存质量...目的:研究早期舌癌局灶性切除术患者给予加味黄连解毒汤治疗后的临床疗效和复发率。方法:将80例早期舌癌局灶性切除术患者随机分为两组。对照组40例,给予常规治疗;研究组40例,给予常规治疗及加味黄连解毒汤治疗。比较两组患者生存质量、缓解程度、不良反应、6～24个月复发率。结果:治疗后,研究组肿瘤缓解率75.00%,明显优于对照组缓解率60.00%,差异具有统计学意义(χ~2=5.128,P=0.024);两组卡氏功能状态(Karnofsky,KPS)评分比较,差异无统计学意义(t=0.149,P=0.149);研究组行为状况评分(performance status,PS)明显优于对照组,差异有统计学意义(t=2.079,P=0.041);研究组生存质量评分(quality of life,QOL)明显优于对照组,差异有统计学意义(t=6.904,P=0.000);研究组不良反应率为20.00%,对照组不良反应率为35.00%,两组比较,差异具有统计学意义(χ~2=5.643,P=0.039)。在6～24个月随访中,研究组有7例患者失访,对照组有5例患者失访,1例死亡,研究组6～24个月的复发率为27.27%(9/33),明显优于对照组的复发率44.12%(15/34),差异具有统计学意义(χ~2=6.185,P=0.013)。结论:早期舌癌局灶性切除术患者进行加味黄连解毒汤治疗,可有效提高患者肿瘤缓解率,减少近期复发率,并改善患者日常生活功能状态,从而提高生存质量。展开更多
基金supported bythe Research Fund from Science and Technology Department of Guangdong Province(No.2014A020212277)the Specific Science and Technology Research Fund from Guangdong Province(No.YN2015QN07)
文摘Background Sepsis is a complex pathophysiologic process, which is the leading cause of death in the world. Many studies have revealed that Chinese medicine has shown tremendous advantages on the treatment of sepsis. Our study aims to explore the effect of Huanglian jiedu decoction and Jiawei huanglian jiedu decoction on organ function in septic rats. Methods SD rats were randomly divided into 7 groups, sham-group, cecal ligation and puncture group （CLP, 18h/72h）, Jiawei huanglian jiedu decoction group （JW, 18h/72h）, Huanglian jiedu decoc-tion group （W, 18h/72h）. Except the Sham group, the rats in other groups received cecal ligation and puncture method to induce sepsis. CLP groups were intragastric administration with normal saline after CLP. Huanglian jiedu decoction and Jiawei huanglian jiedu decoction groups were intragastric administration with Huanglian jie-du decoction and Jiawei huanglian jiedu decoction in the same time points. Glutamic-pyruvic transaminase （ALT）, glutamic oxalacetic transaminase （AST）, total bilirubin （TBIL）, creatinine（Cr）, urea, creatine kinase-MB （CK-MB）, lactate dehydrogenase （LDH） were detected by biochemical analyzer. Brain natriuretic peptide （BNP）, surfactant protein A （SP-A）, chemotactic factor for monocyte-1 （MCP-1）, D-lactic acid （D-LAC）, complement 5a, mobility group box-1 protein B 1 （HMGB-1）, interleukin 6 （IL-6） were analyzed by enzyme-linked immunosor-bent assay （ELISA）. Total lung capacity （TLC） and function residual capacity （FRC） were measured by buxco re-search system. The wet to dry weight ratio （W/D） was tested. Moreover, changes in tissue pathology were evaluat-ed using hematoxylin and eosin （H＆E） staining and electron microscope. Results The level of AST/ALT in se-rum was decreased after treatment with Huanglian jiedu decoction and Jiawei huanglian jiedu decoction. More-over, the level of TLC and FRC was significantly increased, and the level of SP-A, W/D was significantly de-creased
文摘目的:研究早期舌癌局灶性切除术患者给予加味黄连解毒汤治疗后的临床疗效和复发率。方法:将80例早期舌癌局灶性切除术患者随机分为两组。对照组40例,给予常规治疗;研究组40例,给予常规治疗及加味黄连解毒汤治疗。比较两组患者生存质量、缓解程度、不良反应、6～24个月复发率。结果:治疗后,研究组肿瘤缓解率75.00%,明显优于对照组缓解率60.00%,差异具有统计学意义(χ~2=5.128,P=0.024);两组卡氏功能状态(Karnofsky,KPS)评分比较,差异无统计学意义(t=0.149,P=0.149);研究组行为状况评分(performance status,PS)明显优于对照组,差异有统计学意义(t=2.079,P=0.041);研究组生存质量评分(quality of life,QOL)明显优于对照组,差异有统计学意义(t=6.904,P=0.000);研究组不良反应率为20.00%,对照组不良反应率为35.00%,两组比较,差异具有统计学意义(χ~2=5.643,P=0.039)。在6～24个月随访中,研究组有7例患者失访,对照组有5例患者失访,1例死亡,研究组6～24个月的复发率为27.27%(9/33),明显优于对照组的复发率44.12%(15/34),差异具有统计学意义(χ~2=6.185,P=0.013)。结论:早期舌癌局灶性切除术患者进行加味黄连解毒汤治疗,可有效提高患者肿瘤缓解率,减少近期复发率,并改善患者日常生活功能状态,从而提高生存质量。
