Background The two most basic properties of mesenchymal stem cells （MSCs） are the capacities to selfrenew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Whar...Background The two most basic properties of mesenchymal stem cells （MSCs） are the capacities to selfrenew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Wharton＇ s Jelly have properties of MSCs and represent a rich source of primitive cells. This study was conducted to explore the possibility of inducing human umbilical cord Wharton＇ s Jelly-derived MSCs to differentiate into nerve-like cells.Methods MSCs were cultured from the Wharton＇ s Jelly taken from human umbilical cord of babies delivered after full-term normal labor. Salvia miltiorrhiza and [3-mercaptoethanol were used to induce the human umbilical cord-derived MSCs to differentiate The expression of neural protein markers was shown by immunocytochemistry. The induction process was monitored by phase contrast microscopy, electron microscopy （EM）, and laser scanning confocal microscopy （LSCM） . The pleiotrophin and nestin genes were measured by reverse transcription-polymerase chain reaction （RT-PCR）.Results MSCs in the Wharton＇ s Jelly were easily attainable and could be maintained and expanded in culture. They were positive for markers of MSCs, but negative for markers of hematopoietic cells and graft-versus-host disease （GVHD）-related cells. Treatment with Salvia mihiorrhiza caused Wharton＇ s Jelly cells to undergo profound morphological changes. The induced MSCs developed rounded cell bodies with multiple neurite-like extensions. Eventually they developed processes that formed networks reminiscent of primary cultures of neurons. Salvia mihiorrhiza and β-mercaptoethanol also induced MSCs to express nestin, β-tubulin Ⅲ, neurofilament （NF） and glial fibrillary acidic protein （GFAP）. It was confirmed by RT-PCR that MSCs could express pleiotrophin both before and after induction by Salvia miltiorrhiza. The expression was markedly enhanced after induction and the nestin gene was also expressed.Conclusions MSCs could be isolated from human umbilical cord Wharto展开更多
Human umbilical cord(UC)is a promising source of mesenchymal stem cells(MSCs).Apart from their prominent advantages,such as a painless collection procedure and faster self-renewal,UC-MSCs have shown the ability to dif...Human umbilical cord(UC)is a promising source of mesenchymal stem cells(MSCs).Apart from their prominent advantages,such as a painless collection procedure and faster self-renewal,UC-MSCs have shown the ability to differentiate into three germ layers,to accumulate in damaged tissue or inflamed regions,to promote tissue repair,and to modulate immune response.There are diverse protocols and culture methods for the isolation of MSCs from the various compartments of UC,such as Wharton’s jelly,vein,arteries,UC lining and subamnion and perivascular regions.In this review,we give a brief introduction to various compartments of UC as a source of MSCs and emphasize the potential clinical utility of UC-MSCs for regenerative medicine and immunotherapy.展开更多
以兰州百合为主要原料,卡拉胶和魔芋胶为复配凝胶剂研究百合果冻的配方。在单因素实验的基础上,选择复配胶浓度和配比、百合汁和氯化钾添加量为自变量,利用Box-Behnken法进行四因素三水平响应面优化设计实验,确定最优工艺参数为:复配胶2...以兰州百合为主要原料,卡拉胶和魔芋胶为复配凝胶剂研究百合果冻的配方。在单因素实验的基础上,选择复配胶浓度和配比、百合汁和氯化钾添加量为自变量,利用Box-Behnken法进行四因素三水平响应面优化设计实验,确定最优工艺参数为:复配胶2.0%,卡拉胶/魔芋胶为2∶1,百合汁30%,氯化钾0.14%,蔗糖12%,柠檬酸0.12%,制作的果冻弹性为2.80±0.0081 mm,咀嚼性为1.03±0.0067 m J,感官得分为91±0.6710分,具有独特的百合风味。展开更多
在结合传统果冻工艺配方的基础之上,通过添加菊粉和食用菊花,研制了一种新型的、具有功能性的菊粉花茶果冻。采用单因素实验和正交实验,确定了影响菊粉花茶果冻品质的主次因素,依次为氯化钾用量>复配胶总量>柠檬酸用量>白砂糖...在结合传统果冻工艺配方的基础之上,通过添加菊粉和食用菊花,研制了一种新型的、具有功能性的菊粉花茶果冻。采用单因素实验和正交实验,确定了影响菊粉花茶果冻品质的主次因素,依次为氯化钾用量>复配胶总量>柠檬酸用量>白砂糖用量。通过感官评定,确定了菊粉花茶果冻的最佳工艺配方。结果表明:复配胶的最佳配比为:菊粉∶黄原胶∶魔芋粉=3∶5∶2(m/m);菊粉花茶果冻配方为:菊花水100 m L,复配胶1.0 g/100 m L、柠檬酸0.06 g/100 m L、氯化钾0.08 g/100 m L、白砂糖4.0 g/100 m L,所制果冻产品具有质地均匀,外观良好和花香风味等特点。展开更多
文摘Background The two most basic properties of mesenchymal stem cells （MSCs） are the capacities to selfrenew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Wharton＇ s Jelly have properties of MSCs and represent a rich source of primitive cells. This study was conducted to explore the possibility of inducing human umbilical cord Wharton＇ s Jelly-derived MSCs to differentiate into nerve-like cells.Methods MSCs were cultured from the Wharton＇ s Jelly taken from human umbilical cord of babies delivered after full-term normal labor. Salvia miltiorrhiza and [3-mercaptoethanol were used to induce the human umbilical cord-derived MSCs to differentiate The expression of neural protein markers was shown by immunocytochemistry. The induction process was monitored by phase contrast microscopy, electron microscopy （EM）, and laser scanning confocal microscopy （LSCM） . The pleiotrophin and nestin genes were measured by reverse transcription-polymerase chain reaction （RT-PCR）.Results MSCs in the Wharton＇ s Jelly were easily attainable and could be maintained and expanded in culture. They were positive for markers of MSCs, but negative for markers of hematopoietic cells and graft-versus-host disease （GVHD）-related cells. Treatment with Salvia mihiorrhiza caused Wharton＇ s Jelly cells to undergo profound morphological changes. The induced MSCs developed rounded cell bodies with multiple neurite-like extensions. Eventually they developed processes that formed networks reminiscent of primary cultures of neurons. Salvia mihiorrhiza and β-mercaptoethanol also induced MSCs to express nestin, β-tubulin Ⅲ, neurofilament （NF） and glial fibrillary acidic protein （GFAP）. It was confirmed by RT-PCR that MSCs could express pleiotrophin both before and after induction by Salvia miltiorrhiza. The expression was markedly enhanced after induction and the nestin gene was also expressed.Conclusions MSCs could be isolated from human umbilical cord Wharto
文摘Human umbilical cord(UC)is a promising source of mesenchymal stem cells(MSCs).Apart from their prominent advantages,such as a painless collection procedure and faster self-renewal,UC-MSCs have shown the ability to differentiate into three germ layers,to accumulate in damaged tissue or inflamed regions,to promote tissue repair,and to modulate immune response.There are diverse protocols and culture methods for the isolation of MSCs from the various compartments of UC,such as Wharton’s jelly,vein,arteries,UC lining and subamnion and perivascular regions.In this review,we give a brief introduction to various compartments of UC as a source of MSCs and emphasize the potential clinical utility of UC-MSCs for regenerative medicine and immunotherapy.
文摘以兰州百合为主要原料,卡拉胶和魔芋胶为复配凝胶剂研究百合果冻的配方。在单因素实验的基础上,选择复配胶浓度和配比、百合汁和氯化钾添加量为自变量,利用Box-Behnken法进行四因素三水平响应面优化设计实验,确定最优工艺参数为:复配胶2.0%,卡拉胶/魔芋胶为2∶1,百合汁30%,氯化钾0.14%,蔗糖12%,柠檬酸0.12%,制作的果冻弹性为2.80±0.0081 mm,咀嚼性为1.03±0.0067 m J,感官得分为91±0.6710分,具有独特的百合风味。
文摘在结合传统果冻工艺配方的基础之上,通过添加菊粉和食用菊花,研制了一种新型的、具有功能性的菊粉花茶果冻。采用单因素实验和正交实验,确定了影响菊粉花茶果冻品质的主次因素,依次为氯化钾用量>复配胶总量>柠檬酸用量>白砂糖用量。通过感官评定,确定了菊粉花茶果冻的最佳工艺配方。结果表明:复配胶的最佳配比为:菊粉∶黄原胶∶魔芋粉=3∶5∶2(m/m);菊粉花茶果冻配方为:菊花水100 m L,复配胶1.0 g/100 m L、柠檬酸0.06 g/100 m L、氯化钾0.08 g/100 m L、白砂糖4.0 g/100 m L,所制果冻产品具有质地均匀,外观良好和花香风味等特点。
