目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离...目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离体肝脏消耗葡萄糖的影响与肝脏损伤状况。结果受试的中药提取液在120 m in内对灌流液中谷丙转氨酶没有显著性影响,桑白皮、桑枝、桑寄生提取物能显著降低灌流液中葡萄糖的浓度。结论桑白皮、桑寄生、桑枝提取物在胰岛素存在时能增加离体肝脏葡萄糖的消耗。展开更多
Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouselivers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and highspeed centrifugation. SFA was enriched 2...Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouselivers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and highspeed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured byradioimmunoassay. The basic method offers opportunities for further increases of yield andfor the enrichment of SFA uf other vertebrates.展开更多
文摘目的利用大鼠离体肝脏灌流技术评价中药提取物在胰岛素存在时对离体肝组织消耗葡萄糖的影响。方法采用离体肝灌流技术,将中药提取物加入灌流液中,观察灌流液中谷丙转氨酶(ALT)及葡萄糖浓度的变化,用以评价中药提取物在胰岛素存在时对离体肝脏消耗葡萄糖的影响与肝脏损伤状况。结果受试的中药提取液在120 m in内对灌流液中谷丙转氨酶没有显著性影响,桑白皮、桑枝、桑寄生提取物能显著降低灌流液中葡萄糖的浓度。结论桑白皮、桑寄生、桑枝提取物在胰岛素存在时能增加离体肝脏葡萄糖的消耗。
文摘Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouselivers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and highspeed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured byradioimmunoassay. The basic method offers opportunities for further increases of yield andfor the enrichment of SFA uf other vertebrates.
