AIM: To study gastric mucosal interleukine-8 (IL-8) mRNA expression, the cytotoxin-associated gene A (cagA) mutation, and serum pepsinogen (PG)?I/II ratio related risk in Thai gastric cancer.METHODS: There were consen...AIM: To study gastric mucosal interleukine-8 (IL-8) mRNA expression, the cytotoxin-associated gene A (cagA) mutation, and serum pepsinogen (PG)?I/II ratio related risk in Thai gastric cancer.METHODS: There were consent 134 Thai non-cancer volunteers who underwent endoscopic narrow band imaging examination, and 86 Thais advance gastric cancer patients who underwent endoscopic mucosal biopsies and gastric surgery. Tissue samples were taken by endoscopy with 3 points biopsies. The serum PG?I, II, and Helicobacter pylori (H. pylori) immunoglobulin G (IgG) antibody for H. pylori were tested by enzyme-linked immunosorbent assay technique. The histopathology description of gastric cancer and non-cancer with H. pylori detection was defined with modified Sydney Score System. Gastric mucosal tissue H. pylori DNA was extracted and genotyped for cagA mutation. Tissue IL-8 and cyclooxygenase-2 (COX-2) mRNA expression were conducted by real time relative quantitation polymerase chain reaction. From 17 Japanese advance gastric cancer and 12 benign gastric tissue samples, all were tested for genetic expression with same methods as well as Thai gastric mucosal tissue samples. The multivariate analysis was used for the risk study. Correlation and standardized t-test were done for quantitative data, P value < 0.05 was considered as a statistically significant.RESULTS: There is a high non cagA gene of 86.8 per cent in Thai gastric cancer although there are high yields of the East Asian type in the positive cagA. The H. pylori infection prevalence in this study is reported by combined histopathology and H. pylori IgG antibody test with 77.1% and 97.4% of sensitivity and specificity, respectively. The serum PG?I/II ratio in gastric cancer is significantly lower than in the non-cancer group, P = 0.045. The serum PG?I/II ratio of less than 3.0 and IL-8 mRNA expression ≥ 100 or log10 ≥ 2 are significant cut off risk differences between Thai cancer and non-cancer, P = 0.03 and P < 0.001, respectively. There is a significantly lower PG展开更多
AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden...AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden oscopy. Endoscopic and histopathologic diagnosis of reflux esophagitis was established by Savary-Miller and Vandenplas grading systems, respectively. Esophageal biopsy specimens were taken from the esophagus 20% proximal above the esophagogastric junction for conventional histopathological examination and the measurements of ROS and cytokine levels. ROS were measured by chemiluminescence, whereas IL-8 and MCP-1 levels were determined with quantitative immunometric ELISA on esophageal tissue. Esophagealtissue ROS, IL-8 and MCP-1 levels were compared among groups with and without endoscopic/histo- pathologic esophagitis. RESULTS: Of 59 patients 28 (47.5%) had normal esophagus whereas 31 (52.5%) had endoscopic esophagitis. In histopathological evaluation, almost 73% of the cases had mild and 6.8% had moderate degree of esophagitis. When ROS and chemokine levels were compared among groups with and without endoscopic esophagitis, statistical difference could not be found between patients with and without esophagitis. Although the levels of ROS, IL-8 and MCP-1 were found to be higher in the group with histopathological reflux esophagitis, this difference was not statistically significant. CONCLUSION: These results suggest that the grade of esophagitis is usually mild or moderate during childhood and factors apart from ROS, IL-8 and MCP-1 may be involved in the pathogenesis of reflux esophagitis in children.展开更多
目的:观察在常规西医治疗的基础上,痰热清注射液治疗慢性阻塞性肺疾病急性加重期(acute exacerbation of chronic obstructive pul monary disease,AECOPD)痰热阻肺证患者的临床疗效,并从气道炎症和气道黏液高分泌研究清热化痰法治疗AEC...目的:观察在常规西医治疗的基础上,痰热清注射液治疗慢性阻塞性肺疾病急性加重期(acute exacerbation of chronic obstructive pul monary disease,AECOPD)痰热阻肺证患者的临床疗效,并从气道炎症和气道黏液高分泌研究清热化痰法治疗AECOPD痰热阻肺证的机制。方法:选择AECOPD患者90例,将其随机分为痰热清组、盐酸氨溴索组和对照组。对照组仅给予西医基础治疗;痰热清组给予西医基础治疗的同时,静脉滴注痰热清注射液20ml+5%葡萄糖注射液250ml,1次/d;盐酸氨溴索组给予西医基础治疗的同时,静脉滴注盐酸氨溴索注射液15mg+5%葡萄糖注射液100ml,2次/d,疗程均为10d。观察治疗前后中医症状积分、血浆白细胞介素8(interleukin-8,IL-8)、IL-10和中性粒细胞弹性蛋白酶(neutrophil elastase,NE)水平。结果:痰热清注射液可使咳嗽、痰量、咳痰、气喘、发热、舌苔、脉象等中医症状和体征明显改善(P<0.05);其改善咳嗽、痰量和咳痰的疗效明显优于对照组(P<0.05),但与盐酸氨溴索组比较,差异无统计学意义(P>0.05);痰热清组舌苔变化均较盐酸氨溴索组和对照组有所改善(P<0.05)。治疗后,痰热清组及盐酸氨溴索组IL-8、IL-10和NE水平均明显下降(P<0.05),而对照组仅IL-8和IL-10明显下降(P<0.05);痰热清组IL-8治疗前后变化高于其他两组,盐酸氨溴索组IL-10和NE治疗前后的变化高于其他2组,但3组间IL-8、IL-10和NE治疗前后的变化比较,差异均无统计学意义(P>0.05)。3组总显效率比较,痰热清组及盐酸氨溴索组均优于对照组(P<0.05),痰热清组和盐酸氨溴索组显效率比较,差异无统计学意义(P>0.05);3组总有效率比较,差异均无统计学意义(P>0.05)。结论:清热化痰法代表方痰热清注射液在西医的基础治疗上能够较好地改善AECOPD(痰热阻肺证)患者中医症状及体征,其治疗的机制之一可能是通过促进IL-8、NE水平的下降以及延缓IL-10水平下降而改展开更多
基金Supported by JSPS Ronpaku (Dissertation PhD) program (No.NRCT 10726) award by Japan Society for the Promotion of Scince and in collaboration with Kobe University School of Medicine,Kobe,JapanJSPS Asian CORE Program 2012,Nippon Medical Schoolthe Faculty of Medicine,Chiang Mai University,Chiang Mai,Thailand (in part)
文摘AIM: To study gastric mucosal interleukine-8 (IL-8) mRNA expression, the cytotoxin-associated gene A (cagA) mutation, and serum pepsinogen (PG)?I/II ratio related risk in Thai gastric cancer.METHODS: There were consent 134 Thai non-cancer volunteers who underwent endoscopic narrow band imaging examination, and 86 Thais advance gastric cancer patients who underwent endoscopic mucosal biopsies and gastric surgery. Tissue samples were taken by endoscopy with 3 points biopsies. The serum PG?I, II, and Helicobacter pylori (H. pylori) immunoglobulin G (IgG) antibody for H. pylori were tested by enzyme-linked immunosorbent assay technique. The histopathology description of gastric cancer and non-cancer with H. pylori detection was defined with modified Sydney Score System. Gastric mucosal tissue H. pylori DNA was extracted and genotyped for cagA mutation. Tissue IL-8 and cyclooxygenase-2 (COX-2) mRNA expression were conducted by real time relative quantitation polymerase chain reaction. From 17 Japanese advance gastric cancer and 12 benign gastric tissue samples, all were tested for genetic expression with same methods as well as Thai gastric mucosal tissue samples. The multivariate analysis was used for the risk study. Correlation and standardized t-test were done for quantitative data, P value < 0.05 was considered as a statistically significant.RESULTS: There is a high non cagA gene of 86.8 per cent in Thai gastric cancer although there are high yields of the East Asian type in the positive cagA. The H. pylori infection prevalence in this study is reported by combined histopathology and H. pylori IgG antibody test with 77.1% and 97.4% of sensitivity and specificity, respectively. The serum PG?I/II ratio in gastric cancer is significantly lower than in the non-cancer group, P = 0.045. The serum PG?I/II ratio of less than 3.0 and IL-8 mRNA expression ≥ 100 or log10 ≥ 2 are significant cut off risk differences between Thai cancer and non-cancer, P = 0.03 and P < 0.001, respectively. There is a significantly lower PG
基金A Grant Obtained from Marmara University School of Medicine, SAG-TUS-300505-0214
文摘AIM: To determine the role of inflammatory cytokines and reactive oxygen species (ROS) in childhood reflux esophagitis. METHODS: A total of 59 subjects who had complaints suggesting GERD underwent esophagogastroduoden oscopy. Endoscopic and histopathologic diagnosis of reflux esophagitis was established by Savary-Miller and Vandenplas grading systems, respectively. Esophageal biopsy specimens were taken from the esophagus 20% proximal above the esophagogastric junction for conventional histopathological examination and the measurements of ROS and cytokine levels. ROS were measured by chemiluminescence, whereas IL-8 and MCP-1 levels were determined with quantitative immunometric ELISA on esophageal tissue. Esophagealtissue ROS, IL-8 and MCP-1 levels were compared among groups with and without endoscopic/histo- pathologic esophagitis. RESULTS: Of 59 patients 28 (47.5%) had normal esophagus whereas 31 (52.5%) had endoscopic esophagitis. In histopathological evaluation, almost 73% of the cases had mild and 6.8% had moderate degree of esophagitis. When ROS and chemokine levels were compared among groups with and without endoscopic esophagitis, statistical difference could not be found between patients with and without esophagitis. Although the levels of ROS, IL-8 and MCP-1 were found to be higher in the group with histopathological reflux esophagitis, this difference was not statistically significant. CONCLUSION: These results suggest that the grade of esophagitis is usually mild or moderate during childhood and factors apart from ROS, IL-8 and MCP-1 may be involved in the pathogenesis of reflux esophagitis in children.
文摘目的:观察在常规西医治疗的基础上,痰热清注射液治疗慢性阻塞性肺疾病急性加重期(acute exacerbation of chronic obstructive pul monary disease,AECOPD)痰热阻肺证患者的临床疗效,并从气道炎症和气道黏液高分泌研究清热化痰法治疗AECOPD痰热阻肺证的机制。方法:选择AECOPD患者90例,将其随机分为痰热清组、盐酸氨溴索组和对照组。对照组仅给予西医基础治疗;痰热清组给予西医基础治疗的同时,静脉滴注痰热清注射液20ml+5%葡萄糖注射液250ml,1次/d;盐酸氨溴索组给予西医基础治疗的同时,静脉滴注盐酸氨溴索注射液15mg+5%葡萄糖注射液100ml,2次/d,疗程均为10d。观察治疗前后中医症状积分、血浆白细胞介素8(interleukin-8,IL-8)、IL-10和中性粒细胞弹性蛋白酶(neutrophil elastase,NE)水平。结果:痰热清注射液可使咳嗽、痰量、咳痰、气喘、发热、舌苔、脉象等中医症状和体征明显改善(P<0.05);其改善咳嗽、痰量和咳痰的疗效明显优于对照组(P<0.05),但与盐酸氨溴索组比较,差异无统计学意义(P>0.05);痰热清组舌苔变化均较盐酸氨溴索组和对照组有所改善(P<0.05)。治疗后,痰热清组及盐酸氨溴索组IL-8、IL-10和NE水平均明显下降(P<0.05),而对照组仅IL-8和IL-10明显下降(P<0.05);痰热清组IL-8治疗前后变化高于其他两组,盐酸氨溴索组IL-10和NE治疗前后的变化高于其他2组,但3组间IL-8、IL-10和NE治疗前后的变化比较,差异均无统计学意义(P>0.05)。3组总显效率比较,痰热清组及盐酸氨溴索组均优于对照组(P<0.05),痰热清组和盐酸氨溴索组显效率比较,差异无统计学意义(P>0.05);3组总有效率比较,差异均无统计学意义(P>0.05)。结论:清热化痰法代表方痰热清注射液在西医的基础治疗上能够较好地改善AECOPD(痰热阻肺证)患者中医症状及体征,其治疗的机制之一可能是通过促进IL-8、NE水平的下降以及延缓IL-10水平下降而改
