目的:通过观察氧化苦参碱对糖尿病患者空腹血糖、空腹胰岛素、胰岛素抵抗稳态模型指标以及血清中活性氧(reactive oxygen species,R O S)、炎症因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)含量的影响,检测胰岛素抵抗信号通路...目的:通过观察氧化苦参碱对糖尿病患者空腹血糖、空腹胰岛素、胰岛素抵抗稳态模型指标以及血清中活性氧(reactive oxygen species,R O S)、炎症因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)含量的影响,检测胰岛素抵抗信号通路中关键蛋白的含量变化,探究氧化苦参碱对胰岛素抵抗的影响及其机制,为临床上使用氧化苦参碱治疗胰岛素抵抗提供一定的科学依据.方法:采用1∶1随机对照病例研究方法,将研究对象分为氧化苦参治疗组和未治疗对照组,采用葡萄糖氧化酶法检测两组患者空腹血糖、空腹胰岛素水平;比较两组胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)和胰岛素敏感指数(homeostasis model assessment of insulin sensitivity index,HOMA-ISI)采用ELISA检测两组患者血清中ROS、TNF-α含量;采用Western blot检测两组蛋白激酶B(protein kinase B,AKT)、p-AKT、糖原合成酶激酶3α/β(glycogen synthase kinase-3α/β,GSK3α/β)、p-GSK3α/β变化情况.结果:葡萄糖氧化酶实验结果显示,经氧化苦参碱治疗后的患者与对照组相比,空腹血糖、空腹胰岛素显著降低(P<0.05),HOMAI R降低(P<0.05),H O M A-I S I增加(P<0.05);ELISA结果显示,经氧化苦参碱治疗后的患者血清中ROS、TNF-α含量显著低于对照组患者(P<0.05);Western blot结果显示,经氧化苦参碱处理后的肝细胞总蛋白AKT、G S K3α/β含量不变(P>0.05),而p-A K T、p-GSK3α/β均明显增加(P<0.05).结论:氧化苦参碱可降低空腹血糖、空腹胰岛素含量,一方面通过减少ROS、炎症因子的产生,改善胰岛素抵抗;另一方面可以影响胰岛素信号传导通路中关键分子AKT、G S K3α/β的磷酸化,从而改善胰岛素抵抗,这也为临床上使用氧化苦参碱治疗胰岛素抵抗提供了基础依据.展开更多
目的:探讨脂肪因子Vaspin与妊娠期糖尿病(GDM)胰岛素抵抗的内在联系。方法:复苏、传代及诱导分化GDM前脂肪细胞,构建Vaspin过表达载体,转化、培养和提取;以4个过表达梯度(0.0、1.0、2.5、5.0μg)转染传代脂肪细胞,Q-PCR检测Vaspin、胰...目的:探讨脂肪因子Vaspin与妊娠期糖尿病(GDM)胰岛素抵抗的内在联系。方法:复苏、传代及诱导分化GDM前脂肪细胞,构建Vaspin过表达载体,转化、培养和提取;以4个过表达梯度(0.0、1.0、2.5、5.0μg)转染传代脂肪细胞,Q-PCR检测Vaspin、胰岛素受体底物-1/2(IRS-1/2)、磷脂酰肌醇-3激酶(PI3K(P85a))m RNA表达,WB检测Vaspin、IRS-1/2、PI3K(P85a)蛋白表达及IRS-1/2磷酸化水平,[3H]-2-脱氧-D-葡萄糖摄取测定法检测各组葡萄糖摄取率的变化。结果:(1)Vaspin m RNA及蛋白表达随转染浓度梯度升高而表达量增加,所构建Vaspin过表达载体有效;(2)随Vaspin表达增加,IRS-1/2、PI3K(P85a)m RNA、蛋白表达及IRS-1/2酪氨酸磷酸化程度均未出现明显变化;(3)脂肪细胞葡萄糖摄取率与Vaspin过表达浓度变化无明显关系。结论:Vaspin在GDM血清及网膜脂肪组织中的高表达与GDM胰岛素抵抗的发生没有必然联系。展开更多
Aims: To determine correlations of insulin sensitivity to gene expression in omental and subcutaneous adipose tissue of non-obese, non-diabetic pregnant women. Methods: Microarray gene profiling was performed on subcu...Aims: To determine correlations of insulin sensitivity to gene expression in omental and subcutaneous adipose tissue of non-obese, non-diabetic pregnant women. Methods: Microarray gene profiling was performed on subcutaneous and omental adipose tissue from 14 patients and obtained while fasting during non-laboring Cesarean section, using Illumina HumanHT-12 V4 Expression BeadChips. Findings were validated by real-time PCR. Matusda-Insulin sensitivity index (IS) and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated from glucose and insulin levels obtained from a frequently sampled oral glucose tolerance test, and correlated with gene expression. Results: Of genes differentially expressed in omental vs. subcutaneous adipose, in omentum 12 genes were expressed toward insulin resistance, whereas only 5 genes were expressed toward insulin sensitivity. In particular, expression of the insulin receptor gene (INSR), which initiates the insulin signaling cascade, is strongly positively correlated with IS and negatively with HOMA-IR in omental tissue (r = 0.84). Conclusion: Differential gene expression in omentum relative to subcutaneous adipose showed a pro-insulin resistance profile in omentum. A clinical importance of omental adipose is observed here, as downregulation of insulin receptor in omentum is correlated with increased systemic insulin resistance.展开更多
文摘目的:通过观察氧化苦参碱对糖尿病患者空腹血糖、空腹胰岛素、胰岛素抵抗稳态模型指标以及血清中活性氧(reactive oxygen species,R O S)、炎症因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)含量的影响,检测胰岛素抵抗信号通路中关键蛋白的含量变化,探究氧化苦参碱对胰岛素抵抗的影响及其机制,为临床上使用氧化苦参碱治疗胰岛素抵抗提供一定的科学依据.方法:采用1∶1随机对照病例研究方法,将研究对象分为氧化苦参治疗组和未治疗对照组,采用葡萄糖氧化酶法检测两组患者空腹血糖、空腹胰岛素水平;比较两组胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)和胰岛素敏感指数(homeostasis model assessment of insulin sensitivity index,HOMA-ISI)采用ELISA检测两组患者血清中ROS、TNF-α含量;采用Western blot检测两组蛋白激酶B(protein kinase B,AKT)、p-AKT、糖原合成酶激酶3α/β(glycogen synthase kinase-3α/β,GSK3α/β)、p-GSK3α/β变化情况.结果:葡萄糖氧化酶实验结果显示,经氧化苦参碱治疗后的患者与对照组相比,空腹血糖、空腹胰岛素显著降低(P<0.05),HOMAI R降低(P<0.05),H O M A-I S I增加(P<0.05);ELISA结果显示,经氧化苦参碱治疗后的患者血清中ROS、TNF-α含量显著低于对照组患者(P<0.05);Western blot结果显示,经氧化苦参碱处理后的肝细胞总蛋白AKT、G S K3α/β含量不变(P>0.05),而p-A K T、p-GSK3α/β均明显增加(P<0.05).结论:氧化苦参碱可降低空腹血糖、空腹胰岛素含量,一方面通过减少ROS、炎症因子的产生,改善胰岛素抵抗;另一方面可以影响胰岛素信号传导通路中关键分子AKT、G S K3α/β的磷酸化,从而改善胰岛素抵抗,这也为临床上使用氧化苦参碱治疗胰岛素抵抗提供了基础依据.
文摘目的:探讨脂肪因子Vaspin与妊娠期糖尿病(GDM)胰岛素抵抗的内在联系。方法:复苏、传代及诱导分化GDM前脂肪细胞,构建Vaspin过表达载体,转化、培养和提取;以4个过表达梯度(0.0、1.0、2.5、5.0μg)转染传代脂肪细胞,Q-PCR检测Vaspin、胰岛素受体底物-1/2(IRS-1/2)、磷脂酰肌醇-3激酶(PI3K(P85a))m RNA表达,WB检测Vaspin、IRS-1/2、PI3K(P85a)蛋白表达及IRS-1/2磷酸化水平,[3H]-2-脱氧-D-葡萄糖摄取测定法检测各组葡萄糖摄取率的变化。结果:(1)Vaspin m RNA及蛋白表达随转染浓度梯度升高而表达量增加,所构建Vaspin过表达载体有效;(2)随Vaspin表达增加,IRS-1/2、PI3K(P85a)m RNA、蛋白表达及IRS-1/2酪氨酸磷酸化程度均未出现明显变化;(3)脂肪细胞葡萄糖摄取率与Vaspin过表达浓度变化无明显关系。结论:Vaspin在GDM血清及网膜脂肪组织中的高表达与GDM胰岛素抵抗的发生没有必然联系。
文摘Aims: To determine correlations of insulin sensitivity to gene expression in omental and subcutaneous adipose tissue of non-obese, non-diabetic pregnant women. Methods: Microarray gene profiling was performed on subcutaneous and omental adipose tissue from 14 patients and obtained while fasting during non-laboring Cesarean section, using Illumina HumanHT-12 V4 Expression BeadChips. Findings were validated by real-time PCR. Matusda-Insulin sensitivity index (IS) and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated from glucose and insulin levels obtained from a frequently sampled oral glucose tolerance test, and correlated with gene expression. Results: Of genes differentially expressed in omental vs. subcutaneous adipose, in omentum 12 genes were expressed toward insulin resistance, whereas only 5 genes were expressed toward insulin sensitivity. In particular, expression of the insulin receptor gene (INSR), which initiates the insulin signaling cascade, is strongly positively correlated with IS and negatively with HOMA-IR in omental tissue (r = 0.84). Conclusion: Differential gene expression in omentum relative to subcutaneous adipose showed a pro-insulin resistance profile in omentum. A clinical importance of omental adipose is observed here, as downregulation of insulin receptor in omentum is correlated with increased systemic insulin resistance.
