While both induction culture media and matrix have been reported to regulate the stem cell fate,little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the...While both induction culture media and matrix have been reported to regulate the stem cell fate,little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the underlying mechanisms.To this aim,we seeded MSCs on HA-collagen and HA-synthetic hydrogel matrixes,which had demonstrated highly different potentials toward osteoblastic and chondrocytic differentiation lineages,respectively,and cultured them with osteogenic,chondrogenic and normal culture media,respectively.A systematic comparison has been carried out on the effects of induction media and matrix on MSC adhesion,cytoskeleton organization,proliferation,and in particular differentiation into the osteoblastic and chondrocytic lineages.The results demonstrated that the matrix selection had a much more profound effect on directing the differentiation lineage than the induction media did.The strong modulation effect on the transcription activities might be the critical factor contributing to the above observations in our study,where canonical Wnt-b-Catenin signal pathway was directly involved in the matrix-driven osteoblastic differentiation.Such findings not only provide a critical insight on natural cellular events leading to the osteoblastic and chondrocytic differentiations,but also have important implications in biomaterial design for tissue engineering applications.展开更多
The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions p...The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 108 cells/ml was selected because a final spore concentration of 1.05 × 1010 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.展开更多
A comprehensive theoretical study of entropy generation during electroki-netically driven transport of a nanofluid is of prime concern in the paper. The flow is considered to take place on a wavy channel under the act...A comprehensive theoretical study of entropy generation during electroki-netically driven transport of a nanofluid is of prime concern in the paper. The flow is considered to take place on a wavy channel under the action of an external transverse magnetic field and an external pressure gradient. Navier slips at the walls of the channel and thermal radiation have been taken into account in the study. The theoretical study has been carried out by developing a mathematical model by taking into account the effects of Joule heating, viscous dissipation, and the transverse magnetic field on heat transfer during the electrokinetic transport of the fluid. The derived analytical expres-sions have been computed numerically by considering the nanofluid as a mixture of blood and ferromagnetic nanoparticles. Variations in velocity, streaming potential, temperature distribution, Nusselt number, and Bejan number associated with the electrokinetic flow in capillaries have been investigated by the parametric variation method. The results have been presented graphically. The present investigation reveals that streaming potential decreases due to the Hall effect, while for the cooling capacity of the microsystem, we find an opposite behavior due to the Hall effect. The study further reveals that the fluidic temperature is reduced due to increase in the Hall current, and thereby thermal irreversibility of the system is reduced significantly. The results presented here can be considered as the approximate estimates of blood flow dynamics in capillaries during chemotherapy in cancer treatment.展开更多
We present a non-relativistic approach to the equivalent polarization Peq=(1/c2)v×Meq, that appears in a magnetized medium in motion. We apply an analogous method to that used by Panofsky and Phillips to calculat...We present a non-relativistic approach to the equivalent polarization Peq=(1/c2)v×Meq, that appears in a magnetized medium in motion. We apply an analogous method to that used by Panofsky and Phillips to calculate the symmetric effect, the equivalent magnetization that appears in a polarized dielectric in motion, Meq=P×v,?This method is based on a particular expression of Maxwell’s equations and the application of the convective derivative. These authors argue, however, that the equivalent polarization can be obtained only with a relativistic approach. We show that with the same method, but with a different and equivalent expression of Maxwell’s equations, this effect can also be calculated. In this way both effects can be considered relativistic effects to first order in v/c.展开更多
基金The study was supported by the Natural Science Foundation Grants(No.81671826,No.81271702 and No.31600765)Sichuan Province Miaozi Project(2016RZ0032)Sichuan University Start-up Funding(2015SCU11041).
文摘While both induction culture media and matrix have been reported to regulate the stem cell fate,little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the underlying mechanisms.To this aim,we seeded MSCs on HA-collagen and HA-synthetic hydrogel matrixes,which had demonstrated highly different potentials toward osteoblastic and chondrocytic differentiation lineages,respectively,and cultured them with osteogenic,chondrogenic and normal culture media,respectively.A systematic comparison has been carried out on the effects of induction media and matrix on MSC adhesion,cytoskeleton organization,proliferation,and in particular differentiation into the osteoblastic and chondrocytic lineages.The results demonstrated that the matrix selection had a much more profound effect on directing the differentiation lineage than the induction media did.The strong modulation effect on the transcription activities might be the critical factor contributing to the above observations in our study,where canonical Wnt-b-Catenin signal pathway was directly involved in the matrix-driven osteoblastic differentiation.Such findings not only provide a critical insight on natural cellular events leading to the osteoblastic and chondrocytic differentiations,but also have important implications in biomaterial design for tissue engineering applications.
基金the Ministry of Agriculture and Rural Development for the financial support to this project.
文摘The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 108 cells/ml was selected because a final spore concentration of 1.05 × 1010 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.
基金Project supported by Science and Engineering Research Board(SERB)Department of Science and Technology,Government of India,New Delhi(No.CRG/2018/000153)
文摘A comprehensive theoretical study of entropy generation during electroki-netically driven transport of a nanofluid is of prime concern in the paper. The flow is considered to take place on a wavy channel under the action of an external transverse magnetic field and an external pressure gradient. Navier slips at the walls of the channel and thermal radiation have been taken into account in the study. The theoretical study has been carried out by developing a mathematical model by taking into account the effects of Joule heating, viscous dissipation, and the transverse magnetic field on heat transfer during the electrokinetic transport of the fluid. The derived analytical expres-sions have been computed numerically by considering the nanofluid as a mixture of blood and ferromagnetic nanoparticles. Variations in velocity, streaming potential, temperature distribution, Nusselt number, and Bejan number associated with the electrokinetic flow in capillaries have been investigated by the parametric variation method. The results have been presented graphically. The present investigation reveals that streaming potential decreases due to the Hall effect, while for the cooling capacity of the microsystem, we find an opposite behavior due to the Hall effect. The study further reveals that the fluidic temperature is reduced due to increase in the Hall current, and thereby thermal irreversibility of the system is reduced significantly. The results presented here can be considered as the approximate estimates of blood flow dynamics in capillaries during chemotherapy in cancer treatment.
文摘We present a non-relativistic approach to the equivalent polarization Peq=(1/c2)v×Meq, that appears in a magnetized medium in motion. We apply an analogous method to that used by Panofsky and Phillips to calculate the symmetric effect, the equivalent magnetization that appears in a polarized dielectric in motion, Meq=P×v,?This method is based on a particular expression of Maxwell’s equations and the application of the convective derivative. These authors argue, however, that the equivalent polarization can be obtained only with a relativistic approach. We show that with the same method, but with a different and equivalent expression of Maxwell’s equations, this effect can also be calculated. In this way both effects can be considered relativistic effects to first order in v/c.
