利用初步制备的溶藻弧菌外膜蛋白（outer membrane protein of Vibrio alginolyticus，Va-OMP）、溶藻弧菌蛋白分子量58kD外膜蛋白（Va-OMP58）和溶藻弧菌（Vibrio alginolyticus，Va）灭活菌苗、溶藻弧菌脂多糖（lipopolysaccharides o...利用初步制备的溶藻弧菌外膜蛋白（outer membrane protein of Vibrio alginolyticus，Va-OMP）、溶藻弧菌蛋白分子量58kD外膜蛋白（Va-OMP58）和溶藻弧菌（Vibrio alginolyticus，Va）灭活菌苗、溶藻弧菌脂多糖（lipopolysaccharides of Vibrio alginolyticus，Va-LPS）菌苗等进行主动保护性和被动保护性的实验比较，Va-OMP、Va-OMP58免疫组小鼠免疫后用活菌攻击，免疫保护率为62．5％～86．7％，而Va-LPS组和Va灭活菌苗组的免疫保护率为50％～62．5％，对照组的免疫保护率仅为6．7％。用不同抗血清免疫小鼠，活菌攻击后，Va-OMP组存活率达到53．3％和66．7％，Va-OMP58组次之，存活率为40％和50％，Va灭活菌组的存活率为33．3％和46．7％，对照组为13．3％。与对照组相比较，Va-OMP组差异较显著，保护效果较好。溶藻弧菌外膜蛋白和蛋白分子量58kD外膜蛋白的保护性效果高于溶藻弧菌灭活菌苗和溶藻弧菌脂多糖的保护性效果，证明外膜蛋白（outer membrane protein，OMP）具有较强的免疫原性。迟发性超敏反应试验也证明，OMP能诱发变态反应，间接证明OMP具有较强的引起细胞介导免疫反应的能力。因此实验证明，Va-OMP、Va-OMP58是能在小鼠产生体液免疫和细胞介导免疫的保护性抗原。展开更多
Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3. Methods ICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge w...Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3. Methods ICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay, and to measure the secretion of IL-2, IL-4, IL-20, and IFN -y by ELISA. The rate of reduced hydatid cyst and the levels of IgE, igG and IgG subclasses in sera were examined. Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response. The secretion of IFN-V and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-20 levels between vaccinated and control mice. Conclusion The results indicate that the rEg24-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.展开更多
文摘利用初步制备的溶藻弧菌外膜蛋白（outer membrane protein of Vibrio alginolyticus，Va-OMP）、溶藻弧菌蛋白分子量58kD外膜蛋白（Va-OMP58）和溶藻弧菌（Vibrio alginolyticus，Va）灭活菌苗、溶藻弧菌脂多糖（lipopolysaccharides of Vibrio alginolyticus，Va-LPS）菌苗等进行主动保护性和被动保护性的实验比较，Va-OMP、Va-OMP58免疫组小鼠免疫后用活菌攻击，免疫保护率为62．5％～86．7％，而Va-LPS组和Va灭活菌苗组的免疫保护率为50％～62．5％，对照组的免疫保护率仅为6．7％。用不同抗血清免疫小鼠，活菌攻击后，Va-OMP组存活率达到53．3％和66．7％，Va-OMP58组次之，存活率为40％和50％，Va灭活菌组的存活率为33．3％和46．7％，对照组为13．3％。与对照组相比较，Va-OMP组差异较显著，保护效果较好。溶藻弧菌外膜蛋白和蛋白分子量58kD外膜蛋白的保护性效果高于溶藻弧菌灭活菌苗和溶藻弧菌脂多糖的保护性效果，证明外膜蛋白（outer membrane protein，OMP）具有较强的免疫原性。迟发性超敏反应试验也证明，OMP能诱发变态反应，间接证明OMP具有较强的引起细胞介导免疫反应的能力。因此实验证明，Va-OMP、Va-OMP58是能在小鼠产生体液免疫和细胞介导免疫的保护性抗原。
基金supported by National Natural Science Foundation of China (No.30260105 and No.30660176)
文摘Objective To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3. Methods ICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay, and to measure the secretion of IL-2, IL-4, IL-20, and IFN -y by ELISA. The rate of reduced hydatid cyst and the levels of IgE, igG and IgG subclasses in sera were examined. Results Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response. The secretion of IFN-V and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-20 levels between vaccinated and control mice. Conclusion The results indicate that the rEg24-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.
