In this study,egg white peptides(EWP)were prepared using preheat treatment combined with a bienzymatic hydrolysis.Results on peptide yield and molecular weight distribution showed that preheat treatment could obviousl...In this study,egg white peptides(EWP)were prepared using preheat treatment combined with a bienzymatic hydrolysis.Results on peptide yield and molecular weight distribution showed that preheat treatment could obviously promote egg white hydrolysis.The yield of EWP obtained from neutral-alkaline protease treatment was 74.62%±0.23%and 92.69%of EWP was between 200 Da and 500 Da.Supplementation with 750 mg/kg/d EWP significantly alleviated the decrease of immune organ index in immunosuppressed male BLAB/c mice caused by cyclophosphamide.The pathological changes of spleen showed that EWP could also alleviate the damage of spleen tissue.The number of white blood cells in peripheral blood and the levels of serum cytokines(IL-6,IL-2 and TNF-α)in 750 mg/kg/d EWP group were significantly higher than the model group.The immunomodulatory effects of EWP might be related to the presence of abundant branched-chain amino acids,which were important components of immunomodulatory peptides.Eight possible immunomodulatory peptides were identified from EWP by High Performance Liquid Chromatography-Mass Spectrometry in combination with ProteinLynx Global SERVER and mass spectral database.Therefore,EWP may have potential as a natural immunomodulator for the prevention of immune damage caused by external influences.展开更多
Background:Ulcerative colitis(UC)is a chronic disease that often presents with abdominal pain,diarrhea,hematochezia,and significant morbidity.Gancao Xiexin decoction(GXD),a traditional Chinese medicine,has been applie...Background:Ulcerative colitis(UC)is a chronic disease that often presents with abdominal pain,diarrhea,hematochezia,and significant morbidity.Gancao Xiexin decoction(GXD),a traditional Chinese medicine,has been applied for the clinical treatment of UC,while its action mechanisms are unclear.Methods:The active ingredients and their targets of GXD,and UC-related targets,were derived from public databases.Protein-protein interaction,Gene Ontology(GO),and the Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to analyze the important active compounds,key targets,and signaling pathways.Then,molecular docking and animal experiments were performed to verify the findings.A total of 213 active compounds and 89 common targets of GXD for UC were obtained.Results:The hub gene network showed ALB,AKT1,IL6,TNF,VEGFA,TP53,CXCL8,MAPK1,PTGS2,and IL1βmay be potential targets of GXD against UC.GO and KEGG pathway enrichment analyses suggested that the action of GXD against UC was mainly related to response to oxygen levels,lipopolysaccharide,and molecule of bacterial origin,etc.,and achieved by advanced glycation endproducts/receptors for advanced glycation endproducts signaling pathway in diabetic complications,hypoxia-inducible factor(HIF)-1 signaling pathway,interleukin-17/HIF-1 signaling pathway,TNF signaling pathway,etc.Molecular docking results showed that the GXD had good potency of action with the hub target.In vivo experiments showed that GXD significantly alleviated the symptoms of UC and down-regulated the expression of inflammatory factors,nuclear factor-κB and signal transducer and activator of transcription 3.Conclusions:The anti-UC action of GXD is mainly attributed to its anti-oxidative stress,antiinflammatory,and immunomodulatory functions.展开更多
目的探讨纤维连接蛋白（FN）、Ⅰ型胶原蛋白（ColⅠ）两种细胞外基质（ECM）成分对被动致敏的人气道平滑肌细胞（HASMCs）免疫功能的影响及磷脂酰肌醇-3激酶（P13K）在此调控中的作用。方法将HASMCs接种于FN、ColⅠ包被的培养板和空白...目的探讨纤维连接蛋白（FN）、Ⅰ型胶原蛋白（ColⅠ）两种细胞外基质（ECM）成分对被动致敏的人气道平滑肌细胞（HASMCs）免疫功能的影响及磷脂酰肌醇-3激酶（P13K）在此调控中的作用。方法将HASMCs接种于FN、ColⅠ包被的培养板和空白培养板中，用10％哮喘患者血清被动致敏HASMCs，以10％非哮喘者血清为对照，在加入血清前用PBK抑制剂（LY294002）预处理HASMCs 30min。采用RT—PCR法检测HASMCs RANTES（regulated upon activation，normal T cell expressed and secreted）、Eotaxin、TGF—β1 mRNA表达；ELISA法测定HASMCs培养上清中RANTES、Eotaxin、TGF—β1蛋白水平。结果与单纯对照血清组比较，单纯哮喘血清组、对照血清＋FN组、对照血清＋ColⅠ组HASMCs RANTES、Eotaxin、TGF-β1 mRNA和HASMCs培养上清中蛋白的表达均增高（P〈0．05）。哮喘血清＋FN、哮喘血清＋ColⅠ处理HASMC后，RANTES、Eotaxin、TGF—β1 mRNA和HASMCs培养上清中蛋白的表达均高于单纯哮喘血清组，且LY294002干预后上述各指标均下降（P〈0．05）。结论细胞外基质对被动致敏的HASMCs免疫功能具有调控作用，PI3K可能参与细胞外基质对被动致敏的HASMCs的免疫功能调控。展开更多
基金supported by the National Natural Science Foundation for the Youth of China(No.31901642).
文摘In this study,egg white peptides(EWP)were prepared using preheat treatment combined with a bienzymatic hydrolysis.Results on peptide yield and molecular weight distribution showed that preheat treatment could obviously promote egg white hydrolysis.The yield of EWP obtained from neutral-alkaline protease treatment was 74.62%±0.23%and 92.69%of EWP was between 200 Da and 500 Da.Supplementation with 750 mg/kg/d EWP significantly alleviated the decrease of immune organ index in immunosuppressed male BLAB/c mice caused by cyclophosphamide.The pathological changes of spleen showed that EWP could also alleviate the damage of spleen tissue.The number of white blood cells in peripheral blood and the levels of serum cytokines(IL-6,IL-2 and TNF-α)in 750 mg/kg/d EWP group were significantly higher than the model group.The immunomodulatory effects of EWP might be related to the presence of abundant branched-chain amino acids,which were important components of immunomodulatory peptides.Eight possible immunomodulatory peptides were identified from EWP by High Performance Liquid Chromatography-Mass Spectrometry in combination with ProteinLynx Global SERVER and mass spectral database.Therefore,EWP may have potential as a natural immunomodulator for the prevention of immune damage caused by external influences.
文摘Background:Ulcerative colitis(UC)is a chronic disease that often presents with abdominal pain,diarrhea,hematochezia,and significant morbidity.Gancao Xiexin decoction(GXD),a traditional Chinese medicine,has been applied for the clinical treatment of UC,while its action mechanisms are unclear.Methods:The active ingredients and their targets of GXD,and UC-related targets,were derived from public databases.Protein-protein interaction,Gene Ontology(GO),and the Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to analyze the important active compounds,key targets,and signaling pathways.Then,molecular docking and animal experiments were performed to verify the findings.A total of 213 active compounds and 89 common targets of GXD for UC were obtained.Results:The hub gene network showed ALB,AKT1,IL6,TNF,VEGFA,TP53,CXCL8,MAPK1,PTGS2,and IL1βmay be potential targets of GXD against UC.GO and KEGG pathway enrichment analyses suggested that the action of GXD against UC was mainly related to response to oxygen levels,lipopolysaccharide,and molecule of bacterial origin,etc.,and achieved by advanced glycation endproducts/receptors for advanced glycation endproducts signaling pathway in diabetic complications,hypoxia-inducible factor(HIF)-1 signaling pathway,interleukin-17/HIF-1 signaling pathway,TNF signaling pathway,etc.Molecular docking results showed that the GXD had good potency of action with the hub target.In vivo experiments showed that GXD significantly alleviated the symptoms of UC and down-regulated the expression of inflammatory factors,nuclear factor-κB and signal transducer and activator of transcription 3.Conclusions:The anti-UC action of GXD is mainly attributed to its anti-oxidative stress,antiinflammatory,and immunomodulatory functions.
文摘目的探讨纤维连接蛋白（FN）、Ⅰ型胶原蛋白（ColⅠ）两种细胞外基质（ECM）成分对被动致敏的人气道平滑肌细胞（HASMCs）免疫功能的影响及磷脂酰肌醇-3激酶（P13K）在此调控中的作用。方法将HASMCs接种于FN、ColⅠ包被的培养板和空白培养板中，用10％哮喘患者血清被动致敏HASMCs，以10％非哮喘者血清为对照，在加入血清前用PBK抑制剂（LY294002）预处理HASMCs 30min。采用RT—PCR法检测HASMCs RANTES（regulated upon activation，normal T cell expressed and secreted）、Eotaxin、TGF—β1 mRNA表达；ELISA法测定HASMCs培养上清中RANTES、Eotaxin、TGF—β1蛋白水平。结果与单纯对照血清组比较，单纯哮喘血清组、对照血清＋FN组、对照血清＋ColⅠ组HASMCs RANTES、Eotaxin、TGF-β1 mRNA和HASMCs培养上清中蛋白的表达均增高（P〈0．05）。哮喘血清＋FN、哮喘血清＋ColⅠ处理HASMC后，RANTES、Eotaxin、TGF—β1 mRNA和HASMCs培养上清中蛋白的表达均高于单纯哮喘血清组，且LY294002干预后上述各指标均下降（P〈0．05）。结论细胞外基质对被动致敏的HASMCs免疫功能具有调控作用，PI3K可能参与细胞外基质对被动致敏的HASMCs的免疫功能调控。
