Diabetes mellitus and its complications are becoming one of the most important health problems in the world. Diabetic nephropathy is now the main cause of end-stage renal disease. The mechanisms leading tothe develop... Diabetes mellitus and its complications are becoming one of the most important health problems in the world. Diabetic nephropathy is now the main cause of end-stage renal disease. The mechanisms leading tothe development and progression of renal injury are not well known. Therefore, it is very important to f ind new pathogenic pathways to provide opportunities for early diagnosis and targets for novel treatments. At the present time, we know that activation of innate immunity with development of a chronic low grade inflammatory response is a recognized factor in the pathogenesis of diabetic nephropathy. Numerous experimental and clinical studies have shown the participation of different inflammatory molecules and pathways in the pathophysiology of this complication.展开更多
目的探讨重组乙型肝炎(乙肝)疫苗(酵母)[Hepatitis B Vaccine Made by Recombinant DNA Techniques inYeast,HepB(Yeast)]10μg成人免疫失败的因素,为制定成人HepB接种方案提供依据。方法随机选取≥18岁易感人群,按0、1、6个月免疫程序...目的探讨重组乙型肝炎(乙肝)疫苗(酵母)[Hepatitis B Vaccine Made by Recombinant DNA Techniques inYeast,HepB(Yeast)]10μg成人免疫失败的因素,为制定成人HepB接种方案提供依据。方法随机选取≥18岁易感人群,按0、1、6个月免疫程序接种HepB(Yeast)10μg,对免疫失败者进行病例对照研究。结果成人接种10μgHepB(Yeast)免疫失败率为12.99%。免疫失败人群吸烟率、肥胖率、乙肝家族史及微量乙肝病毒(Hepatitis BVirus,HBV)感染率均高于免疫成功人群,差异有显著的统计学意义。结论成人接种10μgHepB(Yeast)免疫失败与吸烟、肥胖、乙肝家族史、微量HBV感染有关。展开更多
目的通过检测宫内感染乙型肝炎病毒(hepatitis B virus,HBV)婴幼儿外周血T细胞亚群的变化,进一步探讨乙型肝炎疫苗免疫失败的机制。方法选择HBV宫内感染免疫失败婴幼儿20例和免疫成功者29例,采用流式细胞术及荧光染色法,检测外周血T细...目的通过检测宫内感染乙型肝炎病毒(hepatitis B virus,HBV)婴幼儿外周血T细胞亚群的变化,进一步探讨乙型肝炎疫苗免疫失败的机制。方法选择HBV宫内感染免疫失败婴幼儿20例和免疫成功者29例,采用流式细胞术及荧光染色法,检测外周血T细胞亚群CD3+、CD4+和CD8+的变化。结果免疫成功组婴幼儿外周血CD3+为64.17%±8.01%,CD4+为39.55%±8.80%,CD8+为24.62%±7.23%;CD4+/CD8+比值为1.66±0.51。免疫失败组婴幼儿外周血CD3+、CD4+和CD8+分别为62.55%±7.32%、32.94%±7.58%和29.61%±7.08%;CD4+/CD8+比值为1.13±0.50。统计分析显示,CD3+T淋巴细胞百分数组间差异无显著性意义(P>0.05),CD4+T和CD8+T细胞在免疫失败组和免疫成功组有显著性差异(P<0.05)。结论婴幼儿外周血CD4+和CD8+T细胞的数量变化可能是乙肝疫苗免疫接种失败的机制之一。展开更多
To investigate the clinical feature, risk factors and outcome of treatment in patients with liver failure complicated by invasive fungal infections. Retro-spective analysis of the clinical data and related factors of ...To investigate the clinical feature, risk factors and outcome of treatment in patients with liver failure complicated by invasive fungal infections. Retro-spective analysis of the clinical data and related factors of 27 patients with liver failure com-plicated by invasive fungal infections was per-formed. These patients were admitted from January 2007 to August 2009 in our department. Among them, Candida albicans accounted for 17cases (54.84%), albicans tropicals for 4 cases (12.90%). Fungal infection in respiratory tract and alimentary tract accounted for 58.06% and 11% respectively. 81.25% of them had fever fluctuating from 37.4oC to 40oC. 81.25% had elevated white blood cell counts .All had the usage of broad-spectrum of antibiotics, whereas some of them used corticosteroids and had invasive medical manipulation for the treatment. Most patients deteriorated after invasive fungal infections. 21 cases accepted with the treatment of antifungal drugs and mortality rate was 63.00%. It was found that the invasive fungal infection possibility of patients with liver failure significantly increased. To prevent the occurrence of invasive fungal infection, promptly early treatment of liver failure, proper use of antibiotics, cautious use or disuse of corticosteroids, reduction of invasive medical manipulation should be well done. Early detection and treatment of fungal infection are vital to decrease in mortality rate.展开更多
IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hour...IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hours, 7 days and 14 days after birth showed level of IgG 1000 mg/dl. This level indicated that they have good passive transfer of immunity. Also, in this study colostrum quality fed to calves was detected using Brix Refractometer. The colostrum is of high quality as it contains 50 mg/ml of IgG. This Brix refractometer can be used on the farm level to estimate colostrum IgG content and monitoring colostrum feeding practices. Colostrum with high quality IgG could provide calves with enough IgG to attain successful passive transfer of immunity. Brix and Obione refractometers provide simple, rapid method for estimating IgG concentration on calf serum, thus considered to be the most common method for determining passive transfer failure.展开更多
基金Supported by Ministerio de Ciencia e Innovación(Instituto de Salud Carlos Ⅲ-Fondo de Investigación Sanitaria:PI07/0870and PI10/576)Ministerio de Sanidad y Política Social(Dirección General de Terapias Avanzadas y Trasplante:TRA-182)Sociedad Espaola de Nefrología y ACINEF
文摘 Diabetes mellitus and its complications are becoming one of the most important health problems in the world. Diabetic nephropathy is now the main cause of end-stage renal disease. The mechanisms leading tothe development and progression of renal injury are not well known. Therefore, it is very important to f ind new pathogenic pathways to provide opportunities for early diagnosis and targets for novel treatments. At the present time, we know that activation of innate immunity with development of a chronic low grade inflammatory response is a recognized factor in the pathogenesis of diabetic nephropathy. Numerous experimental and clinical studies have shown the participation of different inflammatory molecules and pathways in the pathophysiology of this complication.
文摘目的探讨重组乙型肝炎(乙肝)疫苗(酵母)[Hepatitis B Vaccine Made by Recombinant DNA Techniques inYeast,HepB(Yeast)]10μg成人免疫失败的因素,为制定成人HepB接种方案提供依据。方法随机选取≥18岁易感人群,按0、1、6个月免疫程序接种HepB(Yeast)10μg,对免疫失败者进行病例对照研究。结果成人接种10μgHepB(Yeast)免疫失败率为12.99%。免疫失败人群吸烟率、肥胖率、乙肝家族史及微量乙肝病毒(Hepatitis BVirus,HBV)感染率均高于免疫成功人群,差异有显著的统计学意义。结论成人接种10μgHepB(Yeast)免疫失败与吸烟、肥胖、乙肝家族史、微量HBV感染有关。
文摘目的通过检测宫内感染乙型肝炎病毒(hepatitis B virus,HBV)婴幼儿外周血T细胞亚群的变化,进一步探讨乙型肝炎疫苗免疫失败的机制。方法选择HBV宫内感染免疫失败婴幼儿20例和免疫成功者29例,采用流式细胞术及荧光染色法,检测外周血T细胞亚群CD3+、CD4+和CD8+的变化。结果免疫成功组婴幼儿外周血CD3+为64.17%±8.01%,CD4+为39.55%±8.80%,CD8+为24.62%±7.23%;CD4+/CD8+比值为1.66±0.51。免疫失败组婴幼儿外周血CD3+、CD4+和CD8+分别为62.55%±7.32%、32.94%±7.58%和29.61%±7.08%;CD4+/CD8+比值为1.13±0.50。统计分析显示,CD3+T淋巴细胞百分数组间差异无显著性意义(P>0.05),CD4+T和CD8+T细胞在免疫失败组和免疫成功组有显著性差异(P<0.05)。结论婴幼儿外周血CD4+和CD8+T细胞的数量变化可能是乙肝疫苗免疫接种失败的机制之一。
文摘To investigate the clinical feature, risk factors and outcome of treatment in patients with liver failure complicated by invasive fungal infections. Retro-spective analysis of the clinical data and related factors of 27 patients with liver failure com-plicated by invasive fungal infections was per-formed. These patients were admitted from January 2007 to August 2009 in our department. Among them, Candida albicans accounted for 17cases (54.84%), albicans tropicals for 4 cases (12.90%). Fungal infection in respiratory tract and alimentary tract accounted for 58.06% and 11% respectively. 81.25% of them had fever fluctuating from 37.4oC to 40oC. 81.25% had elevated white blood cell counts .All had the usage of broad-spectrum of antibiotics, whereas some of them used corticosteroids and had invasive medical manipulation for the treatment. Most patients deteriorated after invasive fungal infections. 21 cases accepted with the treatment of antifungal drugs and mortality rate was 63.00%. It was found that the invasive fungal infection possibility of patients with liver failure significantly increased. To prevent the occurrence of invasive fungal infection, promptly early treatment of liver failure, proper use of antibiotics, cautious use or disuse of corticosteroids, reduction of invasive medical manipulation should be well done. Early detection and treatment of fungal infection are vital to decrease in mortality rate.
文摘IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hours, 7 days and 14 days after birth showed level of IgG 1000 mg/dl. This level indicated that they have good passive transfer of immunity. Also, in this study colostrum quality fed to calves was detected using Brix Refractometer. The colostrum is of high quality as it contains 50 mg/ml of IgG. This Brix refractometer can be used on the farm level to estimate colostrum IgG content and monitoring colostrum feeding practices. Colostrum with high quality IgG could provide calves with enough IgG to attain successful passive transfer of immunity. Brix and Obione refractometers provide simple, rapid method for estimating IgG concentration on calf serum, thus considered to be the most common method for determining passive transfer failure.
