目的:探讨左归丸对免疫性卵巢早衰小鼠血清性激素及IL-6、IL-17水平的影响。方法:SPF级雌性小鼠60只随机分为对照组、模型组、西药组及左归丸组,除对照组外,所有雌鼠足底、腹部多点皮下注射免疫试剂0.15 m L,14 d后再次皮下注射免疫强...目的:探讨左归丸对免疫性卵巢早衰小鼠血清性激素及IL-6、IL-17水平的影响。方法:SPF级雌性小鼠60只随机分为对照组、模型组、西药组及左归丸组,除对照组外,所有雌鼠足底、腹部多点皮下注射免疫试剂0.15 m L,14 d后再次皮下注射免疫强化试剂0.15 m L,建立免疫性卵巢早衰模型;造模成功第6天,西药组灌胃补佳乐0.15 mg/kg,左归丸组灌胃31.5 g/kg左归丸,对照组和模型组灌胃等量的蒸馏水;各组雌鼠灌胃8周时,取清晨空腹静脉血,ELISA法检测血清中雌激素、卵泡刺激素、黄体生成素,及抗苗勒管激素、抗透明带抗体、基础抑制素B,及IL-6、IL-17水平;摘取雌鼠卵巢及子宫秤取湿重,计算小鼠卵巢系数、子宫系数。结果:与对照组比较,模型组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显降低(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显升高(P<0.01);与模型组比较,西药组及左归丸组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显升高(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显降低(P<0.01);左归丸组上述指标优于西药组(P<0.01)。结论:左归丸有效下调免疫性卵巢早衰小鼠IL-6、IL-17水平,增加生殖器官重量、改善激素水平,从而保护卵巢功能。展开更多
Over the last decades, nitric oxide(NO) has been definitively recognised as one of the key players involved in immunity and inflammation. NO generation was originally described in activated macrophages, which still re...Over the last decades, nitric oxide(NO) has been definitively recognised as one of the key players involved in immunity and inflammation. NO generation was originally described in activated macrophages, which still represent the prototype of NO-producing cells. Notwithstanding, additional cell subsets belonging to both innate and adaptive immunity have been documented to sustain NO propagation by means of the enzymatic activity of different nitric oxide synthase isoforms. Furthermore, due to its chemical characteristics, NO could rapidly react with other free radicals to generate different reactive nitrogen species(RNS), which have been intriguingly associated with many pathological conditions. Nonetheless, the plethora of NO/RNS-mediated effects still remains extremely puzzling. The aim of this manuscript is to dig into the broad literature on the topic to provide intriguing insights on NO-mediated circuits within immune system. We analysed NO and RNS immunological clues arising from their biochemical properties, immunomodulatory activities and finally dealing with their impact on different pathological scenarios with far prompting intriguing perspectives for their pharmacological targeting.展开更多
文摘目的:探讨左归丸对免疫性卵巢早衰小鼠血清性激素及IL-6、IL-17水平的影响。方法:SPF级雌性小鼠60只随机分为对照组、模型组、西药组及左归丸组,除对照组外,所有雌鼠足底、腹部多点皮下注射免疫试剂0.15 m L,14 d后再次皮下注射免疫强化试剂0.15 m L,建立免疫性卵巢早衰模型;造模成功第6天,西药组灌胃补佳乐0.15 mg/kg,左归丸组灌胃31.5 g/kg左归丸,对照组和模型组灌胃等量的蒸馏水;各组雌鼠灌胃8周时,取清晨空腹静脉血,ELISA法检测血清中雌激素、卵泡刺激素、黄体生成素,及抗苗勒管激素、抗透明带抗体、基础抑制素B,及IL-6、IL-17水平;摘取雌鼠卵巢及子宫秤取湿重,计算小鼠卵巢系数、子宫系数。结果:与对照组比较,模型组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显降低(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显升高(P<0.01);与模型组比较,西药组及左归丸组卵巢系数、子宫系数及血清雌激素、抗苗勒管激素、基础抑制素B水平明显升高(P<0.01),卵泡刺激素、黄体生成素及抗透明带抗体、IL-6及IL-17水平明显降低(P<0.01);左归丸组上述指标优于西药组(P<0.01)。结论:左归丸有效下调免疫性卵巢早衰小鼠IL-6、IL-17水平,增加生殖器官重量、改善激素水平,从而保护卵巢功能。
基金supported by the Henan University of Chinese Medicine Doctoral Research Initiation Fund Project(RSBSJJ2020-17)National Key R&D Program-Modernization of Chinese Medicine(2019YFC1708802).
基金Supported by Grant from the Italian Ministry of Health,BANDO GIOVANI RICERCATORI,No.2009-GR-2009-1558698Agnellini AHR was granted by Cariparo Fundation Fellowship
文摘Over the last decades, nitric oxide(NO) has been definitively recognised as one of the key players involved in immunity and inflammation. NO generation was originally described in activated macrophages, which still represent the prototype of NO-producing cells. Notwithstanding, additional cell subsets belonging to both innate and adaptive immunity have been documented to sustain NO propagation by means of the enzymatic activity of different nitric oxide synthase isoforms. Furthermore, due to its chemical characteristics, NO could rapidly react with other free radicals to generate different reactive nitrogen species(RNS), which have been intriguingly associated with many pathological conditions. Nonetheless, the plethora of NO/RNS-mediated effects still remains extremely puzzling. The aim of this manuscript is to dig into the broad literature on the topic to provide intriguing insights on NO-mediated circuits within immune system. We analysed NO and RNS immunological clues arising from their biochemical properties, immunomodulatory activities and finally dealing with their impact on different pathological scenarios with far prompting intriguing perspectives for their pharmacological targeting.
