Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n...Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n situ pistil delivery （ISPDy method with no tissue culture and adaptive to three Brassica species. Briefly this method can be stated as placement of a liquid drop containing binary vector DNA onto the cut surface of emasculated and artificial pollinated Brassica stigma. Transformation rate （Basta-resistant seedlings per 100 pistils） of different species ranged from 0.4% to 16.3%. Factors affecting transforma-tion success included tinning and buffer type. PCR and Southern blot confirmed transgenic events. Reciprocal cross showed reporter gene steadily inherited in nuclear transformed pattern. GUS histochemical assay of transgenic immature seeds showed stained embroys and endosperm in the same seed, indicating DNA delivered through style and integrated into Brassica seed/genome after double fertilization.展开更多
2020年5月,国际腹膜透析学会(International Society for Peritoneal Dialysis,ISPD)发布了《制订高质量、目标导向腹膜透析处方的指南推荐》,本文主要从指南发布的背景、制订高质量腹膜透析处方的推荐意见,以及相关推荐意见在临床应用...2020年5月,国际腹膜透析学会(International Society for Peritoneal Dialysis,ISPD)发布了《制订高质量、目标导向腹膜透析处方的指南推荐》,本文主要从指南发布的背景、制订高质量腹膜透析处方的推荐意见,以及相关推荐意见在临床应用中的问题等方面进行解读,以期为帮助临床腹膜透析专科护理人员更好地了解指南、应用指南,更深入地开展腹膜透析患者管理提供参考。展开更多
文摘Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n situ pistil delivery （ISPDy method with no tissue culture and adaptive to three Brassica species. Briefly this method can be stated as placement of a liquid drop containing binary vector DNA onto the cut surface of emasculated and artificial pollinated Brassica stigma. Transformation rate （Basta-resistant seedlings per 100 pistils） of different species ranged from 0.4% to 16.3%. Factors affecting transforma-tion success included tinning and buffer type. PCR and Southern blot confirmed transgenic events. Reciprocal cross showed reporter gene steadily inherited in nuclear transformed pattern. GUS histochemical assay of transgenic immature seeds showed stained embroys and endosperm in the same seed, indicating DNA delivered through style and integrated into Brassica seed/genome after double fertilization.
文摘2020年5月,国际腹膜透析学会(International Society for Peritoneal Dialysis,ISPD)发布了《制订高质量、目标导向腹膜透析处方的指南推荐》,本文主要从指南发布的背景、制订高质量腹膜透析处方的推荐意见,以及相关推荐意见在临床应用中的问题等方面进行解读,以期为帮助临床腹膜透析专科护理人员更好地了解指南、应用指南,更深入地开展腹膜透析患者管理提供参考。
