本研究探讨用多西环素调控IL 3基因表达的小鼠骨髓基质细胞系对小鼠造血干细胞增殖分化的促进作用。构建含小鼠IL 3基因逆转录病毒载体系统 ,转染小鼠骨髓基质细胞系 ,获得QXMSC1Tet on IL 3;体外加入多西环素诱导IL 3基因表达并检测IL ...本研究探讨用多西环素调控IL 3基因表达的小鼠骨髓基质细胞系对小鼠造血干细胞增殖分化的促进作用。构建含小鼠IL 3基因逆转录病毒载体系统 ,转染小鼠骨髓基质细胞系 ,获得QXMSC1Tet on IL 3;体外加入多西环素诱导IL 3基因表达并检测IL 3表达活性 ;观察细胞培养条件上清液对造血祖细胞集落形成单位的作用及QXMSC1Tet on IL 3与骨髓细胞共培养对造血干细胞增殖分化的影响。结果表明 :多西环素提高了QXMSC1Tet on IL 3细胞系IL 3的表达 ,促进骨髓造血祖细胞克隆形成数 ;与骨髓细胞共培养可促进造血干细胞的增殖分化。结论 :应用多西环素诱导外源基因转染的骨髓基质细胞IL 3的表达 。展开更多
In vitro human monocyteline U937 cells were inoculated with HSV1, which were persistently treated with 10 μg/ml LPS or/and 10 3 U/ml rhGMCSF as well as 10 3 U/ml rhIL3 since two days before inoculation. The effects o...In vitro human monocyteline U937 cells were inoculated with HSV1, which were persistently treated with 10 μg/ml LPS or/and 10 3 U/ml rhGMCSF as well as 10 3 U/ml rhIL3 since two days before inoculation. The effects of GMCSF and IL3 on the resistance of U937 cells to HSV1 were studied by microcytopathy assay for the infective titers of the cell culture supernatans(TCID 50 ). The results showed that at the 4 th day after inoculation the mean titers of GMCSF group and IL3 group were lower 44 and 21 fold than that of control group, respectively. The inhibition of HSV1 replication in LPSstimulated U937 cells induced by GMCSF or by IL3 was more significant. 2, 4, 6 and 8 days after inoculation, the mean titers of GMCSF+LPS group were lower 74, 162, 15 and 11 fold, and those of IL3+LPS group were lower 89, 18, 59 and 10 fold than that of only LPS treated group, respectively. These data indicate that GMCSF and IL3 could antagonise the enhancement activity of LPS for the virus replication in the cells and increase the resistance of U937 cells to HSV1.展开更多
文摘本研究探讨用多西环素调控IL 3基因表达的小鼠骨髓基质细胞系对小鼠造血干细胞增殖分化的促进作用。构建含小鼠IL 3基因逆转录病毒载体系统 ,转染小鼠骨髓基质细胞系 ,获得QXMSC1Tet on IL 3;体外加入多西环素诱导IL 3基因表达并检测IL 3表达活性 ;观察细胞培养条件上清液对造血祖细胞集落形成单位的作用及QXMSC1Tet on IL 3与骨髓细胞共培养对造血干细胞增殖分化的影响。结果表明 :多西环素提高了QXMSC1Tet on IL 3细胞系IL 3的表达 ,促进骨髓造血祖细胞克隆形成数 ;与骨髓细胞共培养可促进造血干细胞的增殖分化。结论 :应用多西环素诱导外源基因转染的骨髓基质细胞IL 3的表达 。
文摘In vitro human monocyteline U937 cells were inoculated with HSV1, which were persistently treated with 10 μg/ml LPS or/and 10 3 U/ml rhGMCSF as well as 10 3 U/ml rhIL3 since two days before inoculation. The effects of GMCSF and IL3 on the resistance of U937 cells to HSV1 were studied by microcytopathy assay for the infective titers of the cell culture supernatans(TCID 50 ). The results showed that at the 4 th day after inoculation the mean titers of GMCSF group and IL3 group were lower 44 and 21 fold than that of control group, respectively. The inhibition of HSV1 replication in LPSstimulated U937 cells induced by GMCSF or by IL3 was more significant. 2, 4, 6 and 8 days after inoculation, the mean titers of GMCSF+LPS group were lower 74, 162, 15 and 11 fold, and those of IL3+LPS group were lower 89, 18, 59 and 10 fold than that of only LPS treated group, respectively. These data indicate that GMCSF and IL3 could antagonise the enhancement activity of LPS for the virus replication in the cells and increase the resistance of U937 cells to HSV1.
