This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery ...This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery of IL-4RA to the airways of mice inhibited elevations of airway responsiveness and the development of allergic inflammation in asthmatic mice, and regulated the Th1/Th2 balance in OVA-sensitized and -challenged mouse models. This suggests that gene therapy is a therapeutic option for treating and controlling chronic airway inflammation and asthma symptoms.展开更多
文摘This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery of IL-4RA to the airways of mice inhibited elevations of airway responsiveness and the development of allergic inflammation in asthmatic mice, and regulated the Th1/Th2 balance in OVA-sensitized and -challenged mouse models. This suggests that gene therapy is a therapeutic option for treating and controlling chronic airway inflammation and asthma symptoms.
文摘目的探讨新疆地区支气管哮喘(Bronchial Asthma,BA)患者白介素-4(IL-4)基因启动子区甲基化的差异。方法选取2014年1月-2016年10月在新疆医科大学第六附属医院呼吸科收治的BA患者80例为试验组,其中男性42例,女性38例,平均年龄(39.2±3.8)岁;选取同期门诊体检健康人群80例为对照组,其中男性44例,女性36例,平均年龄(40.1±2.6)岁;采用甲基化特异性聚合酶链反应(Methylation Specific PCR,MSP)检测IL-4基因甲基化(碱基C经亚硫酸氢盐修饰后变成U,但已甲基化的C则不再改变,据此设计两对不同的引物PCR扩增可检验其差异)。结果与对照组比较,试验组IL-4基因甲基化率较高,差异有统计学意义(P<0.01),试验组IL-4基因启动子区完全甲基化率为42.5%(34/80),未甲基化率为25.0%(20/80),甲基化和未甲基化共存率为32.5%(26/80);对照组IL-4基因启动子区完全甲基化率为11.3%(9/80),未甲基化率为46.3%(37/80);甲基化和未甲基化共存率为42.5%(34/80)。结论 IL-4基因甲基化程度在BA患者发病中有一定作用。
