目的研究白介素29(IL-29)体外抗乙肝病毒的效果。方法从RNA水平探讨了IL-29抗乙肝病毒的效果。RT-PCR分析IL-29诱导抗病毒蛋白MxA、2′,5-′OAS、PKR和RNase L mRNA水平表达;同时Western blot分析IL-29激活的信号通路以探讨其抗乙肝病...目的研究白介素29(IL-29)体外抗乙肝病毒的效果。方法从RNA水平探讨了IL-29抗乙肝病毒的效果。RT-PCR分析IL-29诱导抗病毒蛋白MxA、2′,5-′OAS、PKR和RNase L mRNA水平表达;同时Western blot分析IL-29激活的信号通路以探讨其抗乙肝病毒的机理。结果IL-29能明显降低HepG2.2.15细胞中乙肝病毒mRNA的水平,且能够上调抗病毒蛋白MxA和2′,5-′OAS的mRNA表达并激活ERK1/2、AKT信号途径。结论在HepG2.2.15细胞中IL-29有显著的抗乙肝病毒作用。展开更多
Summary： The aim of this study was to explore the effect of IL-29 on the progression of airway aller- gic disease by detecting the level of IL-29 in airway allergic cell models stimulated by house dust mite （HDM） i...Summary： The aim of this study was to explore the effect of IL-29 on the progression of airway aller- gic disease by detecting the level of IL-29 in airway allergic cell models stimulated by house dust mite （HDM） in the presence or absence of dexamethasone （DEX）. The same batch of human bronchial epithelial cells in exponential growth phase was randomly divided into five groups： blank group （A）, 300 ng/mL HDM group （B）, 1000 ng/mL HDM group （C）, 3000 ng/mL HDM group （D）, and 300 ng/mL HDM＋100 ng/mL DEX group （E）. The IL-29 mRNA expression was detected by quantitative reverse transcription polymerase chain reaction （qRT-PCR）. The IL-29 protein expression in cell sus- pension was detected by ELISA. The results showed that after stimulation with HDM for 24 h, the ex- pression oflL-29 was increased significantly, and after co-stimulation with HDM and DEX for 24 h, the expression oflL-29 in group E was significantly lower than that in the groups stimulated by HDM alone but higher than that in the group A. The differences between the different groups were significant （F=132.957, P〈0.01）. Additionally, the higher the concentration of HDM was, the more significant the increase in the IL-29 expression was. In conclusion, IL-29 may play a role in the progression of airway allergic disease including asthma.展开更多
文摘目的研究白介素29(IL-29)体外抗乙肝病毒的效果。方法从RNA水平探讨了IL-29抗乙肝病毒的效果。RT-PCR分析IL-29诱导抗病毒蛋白MxA、2′,5-′OAS、PKR和RNase L mRNA水平表达;同时Western blot分析IL-29激活的信号通路以探讨其抗乙肝病毒的机理。结果IL-29能明显降低HepG2.2.15细胞中乙肝病毒mRNA的水平,且能够上调抗病毒蛋白MxA和2′,5-′OAS的mRNA表达并激活ERK1/2、AKT信号途径。结论在HepG2.2.15细胞中IL-29有显著的抗乙肝病毒作用。
文摘Summary： The aim of this study was to explore the effect of IL-29 on the progression of airway aller- gic disease by detecting the level of IL-29 in airway allergic cell models stimulated by house dust mite （HDM） in the presence or absence of dexamethasone （DEX）. The same batch of human bronchial epithelial cells in exponential growth phase was randomly divided into five groups： blank group （A）, 300 ng/mL HDM group （B）, 1000 ng/mL HDM group （C）, 3000 ng/mL HDM group （D）, and 300 ng/mL HDM＋100 ng/mL DEX group （E）. The IL-29 mRNA expression was detected by quantitative reverse transcription polymerase chain reaction （qRT-PCR）. The IL-29 protein expression in cell sus- pension was detected by ELISA. The results showed that after stimulation with HDM for 24 h, the ex- pression oflL-29 was increased significantly, and after co-stimulation with HDM and DEX for 24 h, the expression oflL-29 in group E was significantly lower than that in the groups stimulated by HDM alone but higher than that in the group A. The differences between the different groups were significant （F=132.957, P〈0.01）. Additionally, the higher the concentration of HDM was, the more significant the increase in the IL-29 expression was. In conclusion, IL-29 may play a role in the progression of airway allergic disease including asthma.
