Objective:To observe the dynamic changes of interleukin--6(IL--6) and its effects on catabolism ofthe skeletal muscle protein in the early stage after scalding. Methods: After a model of Balb/C mice inflictedwith 18%-...Objective:To observe the dynamic changes of interleukin--6(IL--6) and its effects on catabolism ofthe skeletal muscle protein in the early stage after scalding. Methods: After a model of Balb/C mice inflictedwith 18%-- 20% TBSA full--thickness skin scald on the back and one hindleg was established, the proteincatabolic rate (TNR) and the level of 11.M6 in soleus muscle and serum were determined and the effects of IL6 on TNR of normal mice and those of 11.--6 antiserurn on TNR of the scalded mice wee observed during thefirst week after scalding. Results: The level of IL--6 was significantly increased and maintained during thefirst three days. TNR of the scalded leg was elevated to the peak on the 3rd day and then decreased graduallywhile that of the unscalded leg was increased only on the 2nd day after scalding. TNR was significantlyincreased when IL--6 was injected to the normal mice and IL--6 antiserum could significantly inhibit the. increased TNR of the scalded mice when it was injected intraperitoneally. Conclusion: The increase of IL--6 isrelated with local inflammatory response in the early stage after scalding and the high level of IL--6 canincrease the catabolic rate of muscle protein.展开更多
In an era where the health-damaging effects of wheat flour (gluten) are increasingly recognized, rice flour has become an important alternative for many people, yet its psychophysiological effects remain largely unkno...In an era where the health-damaging effects of wheat flour (gluten) are increasingly recognized, rice flour has become an important alternative for many people, yet its psychophysiological effects remain largely unknown. Here, we report the potential beneficial effects of rice flour for sleep disturbances in stressed mice. Four-week-old male ddY mice were reared in social isolation for 4 weeks. The control group was reared in a social group. Rice flour was given orally in food to isolated mice at a dose of 2.5 w/w% and 5.0 w/w% for 4 weeks from the start of isolation rearing. MF food was given to the control group. Pentobarbital-induced (40 mg/kg, i.p.) time to sleep induction and righting reflex was measured to determine the effects of rice flour on sleep behavior. Blood samples were obtained after the experiments, and serum corticosterone was measured. Sections from the prefrontal cortex and the brainstem were isolated to measure serotonin, dopamine and interleukin (IL)-6 concentrations. We found that the administration of rice flour dose-dependently improved time to sleep and reduced sleep time in socially isolated mice. Blood corticosterone concentrations, which increased after isolation stress, were decreased after the administration of rice flour. Serotonin and dopamine concentrations in the prefrontal cortex which decreased after isolation stress improved after the administration of rice flour. Brainstem IL-6 concentrations increased after isolation stress, but decreased dose-dependently after rice flour administration. Our results suggest that rice flour reverses sleep disturbances in mice induced by social isolation.展开更多
文摘Objective:To observe the dynamic changes of interleukin--6(IL--6) and its effects on catabolism ofthe skeletal muscle protein in the early stage after scalding. Methods: After a model of Balb/C mice inflictedwith 18%-- 20% TBSA full--thickness skin scald on the back and one hindleg was established, the proteincatabolic rate (TNR) and the level of 11.M6 in soleus muscle and serum were determined and the effects of IL6 on TNR of normal mice and those of 11.--6 antiserurn on TNR of the scalded mice wee observed during thefirst week after scalding. Results: The level of IL--6 was significantly increased and maintained during thefirst three days. TNR of the scalded leg was elevated to the peak on the 3rd day and then decreased graduallywhile that of the unscalded leg was increased only on the 2nd day after scalding. TNR was significantlyincreased when IL--6 was injected to the normal mice and IL--6 antiserum could significantly inhibit the. increased TNR of the scalded mice when it was injected intraperitoneally. Conclusion: The increase of IL--6 isrelated with local inflammatory response in the early stage after scalding and the high level of IL--6 canincrease the catabolic rate of muscle protein.
文摘In an era where the health-damaging effects of wheat flour (gluten) are increasingly recognized, rice flour has become an important alternative for many people, yet its psychophysiological effects remain largely unknown. Here, we report the potential beneficial effects of rice flour for sleep disturbances in stressed mice. Four-week-old male ddY mice were reared in social isolation for 4 weeks. The control group was reared in a social group. Rice flour was given orally in food to isolated mice at a dose of 2.5 w/w% and 5.0 w/w% for 4 weeks from the start of isolation rearing. MF food was given to the control group. Pentobarbital-induced (40 mg/kg, i.p.) time to sleep induction and righting reflex was measured to determine the effects of rice flour on sleep behavior. Blood samples were obtained after the experiments, and serum corticosterone was measured. Sections from the prefrontal cortex and the brainstem were isolated to measure serotonin, dopamine and interleukin (IL)-6 concentrations. We found that the administration of rice flour dose-dependently improved time to sleep and reduced sleep time in socially isolated mice. Blood corticosterone concentrations, which increased after isolation stress, were decreased after the administration of rice flour. Serotonin and dopamine concentrations in the prefrontal cortex which decreased after isolation stress improved after the administration of rice flour. Brainstem IL-6 concentrations increased after isolation stress, but decreased dose-dependently after rice flour administration. Our results suggest that rice flour reverses sleep disturbances in mice induced by social isolation.
