The existence of hybrid vigour with high heritability in vegetative traits of Gongronema latifolia will increase its productivity. This study was therefore undertaken to estimate heterosis and heritability in the vege...The existence of hybrid vigour with high heritability in vegetative traits of Gongronema latifolia will increase its productivity. This study was therefore undertaken to estimate heterosis and heritability in the vegetative traits of G. latifolia. Stem cuttings of five heterozygous parents of different geographical origin, and seeds of 10 hybrids resulting from their crosses were laid out in a randomized complete block design with three replications in a field. Data on the vegetative traits were obtained at the maximum growth period in each year of study and was used to estimate heterosis and heritability over the years. Better parent heterosis (BPH) ranged from -72% to 78% over three years. All the vegetative traits—with the exception of cordate base size—showed positive heterosis in a minimum of four cross combinations in two years. The cross AKS-33-EKPENE EDIENE X ANS-53-AWKA gave a higher BPH ranging from 5% to 6% in leaf area in the three years. The crosses ENS-48-MBU X IMS-50-NJIABA and ENS-48-MBU X ANS-53-AWKA gave higher BPH for leaf length and breadth for the first two years. All crosses showed positive and negative BPH for number of vines/plant and cordate base size, respectively. The hybrids ENS-48-MBU X IMS-50-NJIABA and EBS-49-ISHIAGU X IMS-50-NJIABA, with highly heritable BPH > 60 cm2 leaf area, will be selected in favour of other hybrids and parents. The selection of these hybrids will improve and maintain productivity in the species as the leaves are the harvestable part and the species is vegetatively propagated.展开更多
Heatmap cluster figures are often used to represent data sets in the?omic sciences. The default option of the frequently used R heatmap function is to cluster data according to Euclidean distance, which groups data ma...Heatmap cluster figures are often used to represent data sets in the?omic sciences. The default option of the frequently used R heatmap function is to cluster data according to Euclidean distance, which groups data mainly to their numerical value and not to its relative behaviour. The disadvantage of using the default clustering?dendrograms of R is demonstrated. Instead, a script is provided that uses correlation as distance function, which better reveals biologically meaningful information. This optimized script was used to detect heterotic groups in Vitamaize hybrids (purple maize with high nutraceutical value). A field trial with different genetic combinations was performed through an agricultural phenomics approach (holistic evaluation of the phenotype). The grain yield data and other phenotypic variables were represented through heatmap figures. In the data set of Mexican tropical maize germplasm, at least three heterotic groups were detected, in contrast to only two heterotic groups reported earlier in temperate yellow maize from USA and Europe. This optimized script for heatmap correlation bicluster can also be used to better represent metabolomic fingerprints and transcriptomic data sets.展开更多
文摘The existence of hybrid vigour with high heritability in vegetative traits of Gongronema latifolia will increase its productivity. This study was therefore undertaken to estimate heterosis and heritability in the vegetative traits of G. latifolia. Stem cuttings of five heterozygous parents of different geographical origin, and seeds of 10 hybrids resulting from their crosses were laid out in a randomized complete block design with three replications in a field. Data on the vegetative traits were obtained at the maximum growth period in each year of study and was used to estimate heterosis and heritability over the years. Better parent heterosis (BPH) ranged from -72% to 78% over three years. All the vegetative traits—with the exception of cordate base size—showed positive heterosis in a minimum of four cross combinations in two years. The cross AKS-33-EKPENE EDIENE X ANS-53-AWKA gave a higher BPH ranging from 5% to 6% in leaf area in the three years. The crosses ENS-48-MBU X IMS-50-NJIABA and ENS-48-MBU X ANS-53-AWKA gave higher BPH for leaf length and breadth for the first two years. All crosses showed positive and negative BPH for number of vines/plant and cordate base size, respectively. The hybrids ENS-48-MBU X IMS-50-NJIABA and EBS-49-ISHIAGU X IMS-50-NJIABA, with highly heritable BPH > 60 cm2 leaf area, will be selected in favour of other hybrids and parents. The selection of these hybrids will improve and maintain productivity in the species as the leaves are the harvestable part and the species is vegetatively propagated.
文摘Heatmap cluster figures are often used to represent data sets in the?omic sciences. The default option of the frequently used R heatmap function is to cluster data according to Euclidean distance, which groups data mainly to their numerical value and not to its relative behaviour. The disadvantage of using the default clustering?dendrograms of R is demonstrated. Instead, a script is provided that uses correlation as distance function, which better reveals biologically meaningful information. This optimized script was used to detect heterotic groups in Vitamaize hybrids (purple maize with high nutraceutical value). A field trial with different genetic combinations was performed through an agricultural phenomics approach (holistic evaluation of the phenotype). The grain yield data and other phenotypic variables were represented through heatmap figures. In the data set of Mexican tropical maize germplasm, at least three heterotic groups were detected, in contrast to only two heterotic groups reported earlier in temperate yellow maize from USA and Europe. This optimized script for heatmap correlation bicluster can also be used to better represent metabolomic fingerprints and transcriptomic data sets.
