OBJECTIVE： The aim of this study was to evaluate the effectiveness and safety of stem cell transplantation for spinal cord injury（SCI）.DATA SOURCES： PubM ed, EMBASE, Cochrane, China National Knowledge Infrastructu...OBJECTIVE： The aim of this study was to evaluate the effectiveness and safety of stem cell transplantation for spinal cord injury（SCI）.DATA SOURCES： PubM ed, EMBASE, Cochrane, China National Knowledge Infrastructure, China Science and Technology Journal, Wanfang, and Sino Med databases were systematically searched by computer to select clinical randomized controlled trials using stem cell transplantation to treat SCI, published between each database initiation and July 2016. DATA SELECTION： Randomized controlled trials comparing stem cell transplantation with rehabilitation treatment for patients with SCI. Inclusion criteria：（1） Patients with SCI diagnosed according to the American Spinal Injury Association（ASIA） International standards for neurological classification of SCI;（2） patients with SCI who received only stem cell transplantation therapy or stem cell transplantation combined with rehabilitation therapy;（3） one or more of the following outcomes reported： outcomes concerning neurological function including sensory function and locomotor function, activities of daily living, urination functions, and severity of SCI or adverse effects. Studies comprising patients with complications, without full-text, and preclinical animal models were excluded. Quality of the included studies was evaluated using the Cochrane risk of bias assessment tool and Rev Man V5.3 software, provided by the Cochrane Collaboration, was used to perform statistical analysis. OUTCOME MEASURES： ASIA motor score, ASIA light touch score, ASIA pinprick score, ASIA impairment scale grading improvement rate, activities of daily living score, residual urine volume, and adverse events.RESULTS： Ten studies comprising 377 patients were included in the analysis and the overall risk of bias was relatively low level. Four studies did not detail how random sequences were generated, two studies did not clearly state the blinding outcome assessment, two studies lacked blinding outcome assessment, one study lacked follow-展开更多
目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0...目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。展开更多
基金supported by the National Natural Science Foundation of China,No.81273775
文摘OBJECTIVE： The aim of this study was to evaluate the effectiveness and safety of stem cell transplantation for spinal cord injury（SCI）.DATA SOURCES： PubM ed, EMBASE, Cochrane, China National Knowledge Infrastructure, China Science and Technology Journal, Wanfang, and Sino Med databases were systematically searched by computer to select clinical randomized controlled trials using stem cell transplantation to treat SCI, published between each database initiation and July 2016. DATA SELECTION： Randomized controlled trials comparing stem cell transplantation with rehabilitation treatment for patients with SCI. Inclusion criteria：（1） Patients with SCI diagnosed according to the American Spinal Injury Association（ASIA） International standards for neurological classification of SCI;（2） patients with SCI who received only stem cell transplantation therapy or stem cell transplantation combined with rehabilitation therapy;（3） one or more of the following outcomes reported： outcomes concerning neurological function including sensory function and locomotor function, activities of daily living, urination functions, and severity of SCI or adverse effects. Studies comprising patients with complications, without full-text, and preclinical animal models were excluded. Quality of the included studies was evaluated using the Cochrane risk of bias assessment tool and Rev Man V5.3 software, provided by the Cochrane Collaboration, was used to perform statistical analysis. OUTCOME MEASURES： ASIA motor score, ASIA light touch score, ASIA pinprick score, ASIA impairment scale grading improvement rate, activities of daily living score, residual urine volume, and adverse events.RESULTS： Ten studies comprising 377 patients were included in the analysis and the overall risk of bias was relatively low level. Four studies did not detail how random sequences were generated, two studies did not clearly state the blinding outcome assessment, two studies lacked blinding outcome assessment, one study lacked follow-
文摘目的探讨血清外泌体源性circ_0009362对人骨髓间充干细胞(human bone marrow mesenchymalstem cells,hBMSCs)成骨分化的影响及潜在作用机制。方法收集骨质疏松症(osteoporosis,OP)患者的血清并分离外泌体,使用qRT-PCR检测外泌体中circ_0009362的表达水平。诱导hBMSCs成骨并检测细胞中circ_0009362、miR-29b-3p的表达。双荧光素酶报告实验检测circ_0009362、miR-29b-3p的相互调控关系。碱性磷酸酶(ALP)试剂盒检测ALP活性,茜素红(ARS)染色法检测钙沉积。结果与健康对照组相比,OP患者的血清外泌体中circ_0009362表达升高,诱导hBMSCs成骨后circ_0009362的表达降低(均P<0.05)。外泌体能降低hBMSCs中ALP活性与钙沉积百分比,该作用通过分泌circ_0009362实现。敲减外泌体中circ_0009362表达能部分抵消外泌体的作用(均P<0.05)。诱导hBMSCs成骨后miR-29b-3p的表达提高(P<0.05)。circ_0009362与miR-29b-3p存在靶向关系,外泌体通过分泌circ_0009362抑制miR-29b-3p的表达。过表达miR-29b-3p促进hBMSCs中ALP活性与钙沉积百分比,该作用被外泌体部分抵消(均P<0.05)。结论OP患者的血清外泌体通过分泌circ_0009362下调miR-29b-3p的表达来抑制hBMSCs成骨分化。
