The toxicity of ionic liquids（ILs） on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate（[C8mim]NO3） on...The toxicity of ionic liquids（ILs） on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate（[C8mim]NO3） on the microbial populations（bacteria, fungi, and actinomycetes）, soil enzyme（urease, dehydrogenase, acid phosphatase, and β-glucosidase）activities, microbial community diversity using terminal restriction fragment length polymorphism（T-RFLP）, and abundance of the ammonia monooxygenase（amoA） genes of ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） using quantitative real-time polymerase chain reaction（q-PCR） in brown soil at each trial with doses of 0, 1.0, 5.0,and 10.0 mg/kg on days 10, 20, 30, and 40. The contents of [C8mim]NO3 in soil were measured using high performance liquid chromatography with recoveries of 84.3% to 85.2%, and changed less than 10% during the experimental period. A significant decrease was observed from the bacteria, fungi and actinomycetes populations at 10.0 mg/kg, at which the urease activity was inhibited and the β-glucosidase activity was stimulated on days 20, 30, and 40. In addition, [C8mim]NO3 inhibited the dehydrogenase activity at 10 mg/kg on days 30 and 40 and the acid phosphatase activity on day 20. The diversity of the soil microbial community and the gene abundance of AOA-and AOB-amo A were also inhibited. Furthermore, the present investigation provided more scientific information for the toxicity evaluation of ILs in soil.展开更多
为考察盐酸氨溴索注射液在辐照环境中的稳定性,将注射液分别经60Coγ射线(吸收剂量2、4、8、16、26、34 k Gy)辐照、光照(4 500±500)lx、紫外线(UV)辐照、高温(40、60℃)处理10 d,以颜色、澄明度、盐酸氨溴索浓度、有关物质为指标...为考察盐酸氨溴索注射液在辐照环境中的稳定性,将注射液分别经60Coγ射线(吸收剂量2、4、8、16、26、34 k Gy)辐照、光照(4 500±500)lx、紫外线(UV)辐照、高温(40、60℃)处理10 d,以颜色、澄明度、盐酸氨溴索浓度、有关物质为指标,考察其稳定性。结果表明:注射液在γ射线辐照、光照、UV、60℃高温环境中盐酸氨溴索浓度显著下降(p<0.01);且注射液经γ射线辐照、UV辐照后降解生成多种极性较大的未知物质,颜色变为红棕色;盐酸氨溴索浓度在各种处理后的储存过程中仍会持续下降。研究结果表明,盐酸氨溴索注射液在γ射线辐照、UV环境中极不稳定,且在两种环境中产生的效应相近。展开更多
基金supported by the National Key Research and Development Plan (Nos. 2017YFD0200307, 2016YFD0800202 and 2016YFD0201203)the National Natural Science Foundation of China (Nos. 41771282, 41671320)+1 种基金the Natural Science Foundation of Shandong Province, China (No. ZR2017MD005)the Special Funds of Taishan Scholar of Shandong Province, China
文摘The toxicity of ionic liquids（ILs） on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate（[C8mim]NO3） on the microbial populations（bacteria, fungi, and actinomycetes）, soil enzyme（urease, dehydrogenase, acid phosphatase, and β-glucosidase）activities, microbial community diversity using terminal restriction fragment length polymorphism（T-RFLP）, and abundance of the ammonia monooxygenase（amoA） genes of ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） using quantitative real-time polymerase chain reaction（q-PCR） in brown soil at each trial with doses of 0, 1.0, 5.0,and 10.0 mg/kg on days 10, 20, 30, and 40. The contents of [C8mim]NO3 in soil were measured using high performance liquid chromatography with recoveries of 84.3% to 85.2%, and changed less than 10% during the experimental period. A significant decrease was observed from the bacteria, fungi and actinomycetes populations at 10.0 mg/kg, at which the urease activity was inhibited and the β-glucosidase activity was stimulated on days 20, 30, and 40. In addition, [C8mim]NO3 inhibited the dehydrogenase activity at 10 mg/kg on days 30 and 40 and the acid phosphatase activity on day 20. The diversity of the soil microbial community and the gene abundance of AOA-and AOB-amo A were also inhibited. Furthermore, the present investigation provided more scientific information for the toxicity evaluation of ILs in soil.
文摘为考察盐酸氨溴索注射液在辐照环境中的稳定性,将注射液分别经60Coγ射线(吸收剂量2、4、8、16、26、34 k Gy)辐照、光照(4 500±500)lx、紫外线(UV)辐照、高温(40、60℃)处理10 d,以颜色、澄明度、盐酸氨溴索浓度、有关物质为指标,考察其稳定性。结果表明:注射液在γ射线辐照、光照、UV、60℃高温环境中盐酸氨溴索浓度显著下降(p<0.01);且注射液经γ射线辐照、UV辐照后降解生成多种极性较大的未知物质,颜色变为红棕色;盐酸氨溴索浓度在各种处理后的储存过程中仍会持续下降。研究结果表明,盐酸氨溴索注射液在γ射线辐照、UV环境中极不稳定,且在两种环境中产生的效应相近。
