为了利用蚕丝开发天然医用止血材料,以Ca Cl2-乙醇-水三元体系在不同温度条件下水解丝素蛋白并经喷雾干燥制备丝素肽,通过凝胶色谱、红外光谱、扫描电子显微镜、高效液相色谱仪等观测丝素肽的分子质量分布、形貌结构以及氨基酸组成和含...为了利用蚕丝开发天然医用止血材料,以Ca Cl2-乙醇-水三元体系在不同温度条件下水解丝素蛋白并经喷雾干燥制备丝素肽,通过凝胶色谱、红外光谱、扫描电子显微镜、高效液相色谱仪等观测丝素肽的分子质量分布、形貌结构以及氨基酸组成和含量,通过凝血弹性描记分析（TEG）、大鼠肝脏止血模型和体外细胞毒性实验评价丝素肽的止血性能及细胞相容性。丝素蛋白在48℃条件下水解24 h获得的丝素肽分子质量分布在12-76 k D范围,平均分子质量为30 k D;在98℃条件下水解24 h获得的丝素肽分子质量分布在3.9-32.0 k D范围,平均分子质量为12 k D。2种丝素肽的二级结构均以无规卷曲为主,平均分子质量为30 k D的丝素肽存在部分β-折叠结构,且在水中易形成凝胶,用于处理大鼠肝脏止血模型时的出血量少,止血时间短,止血效果优于平均分子质量为12 k D的丝素肽,与Arista止血粉相近,且无细胞毒性,有望开发为体外快速止血材料。展开更多
This study aims to develop collagen-based hemostatic materials. The sheet-shaped collagen sponge was manufactured by freeze-vacuum drying the aqueous solution of collagen (Col) and heat-denatured collagen (Col’) at a...This study aims to develop collagen-based hemostatic materials. The sheet-shaped collagen sponge was manufactured by freeze-vacuum drying the aqueous solution of collagen (Col) and heat-denatured collagen (Col’) at a composition ratio of 2/1. The both sides or one side of sponge was treated with ultraviolet (UV) irradiation for 15 minutes to introduce intermolecular crosslinks between collagen molecules. The elution behavior of collagen sponge was investigated by immersing the sponge in water for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge showed very slow elution properties. On the other hand, the single-sided UV-irradiated sponge showed initially fast elution and subsequent very slow elution properties. Such initially fast elution of collagen molecules from the surface without UV-irradiation allows an adhesion of collagen sponge to the wound surface and results in hemostatic effect. In addition, the water absorption and retention properties of sponge were investigated by placing the hydrated sponge on a mesh for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge absorbed 81 times more water than own weight and showed a value of 45 times even after 7 days. The single-sided UV-irradiated sponge absorbed 80 times more water than own weight and showed a value of 39 times even after 7 days. The sponge with high water absorption and retention properties allows a wound healing effect because such sponge can absorb large amounts of blood plasma and exudates containing various cell growth factors. The double-sided UV-irradiated sponge is a good candidate for the wound dressing. On the other hand, the single-sided UV-irradiated sponge is a good candidate for the hemostatic material.展开更多
文摘为了利用蚕丝开发天然医用止血材料,以Ca Cl2-乙醇-水三元体系在不同温度条件下水解丝素蛋白并经喷雾干燥制备丝素肽,通过凝胶色谱、红外光谱、扫描电子显微镜、高效液相色谱仪等观测丝素肽的分子质量分布、形貌结构以及氨基酸组成和含量,通过凝血弹性描记分析（TEG）、大鼠肝脏止血模型和体外细胞毒性实验评价丝素肽的止血性能及细胞相容性。丝素蛋白在48℃条件下水解24 h获得的丝素肽分子质量分布在12-76 k D范围,平均分子质量为30 k D;在98℃条件下水解24 h获得的丝素肽分子质量分布在3.9-32.0 k D范围,平均分子质量为12 k D。2种丝素肽的二级结构均以无规卷曲为主,平均分子质量为30 k D的丝素肽存在部分β-折叠结构,且在水中易形成凝胶,用于处理大鼠肝脏止血模型时的出血量少,止血时间短,止血效果优于平均分子质量为12 k D的丝素肽,与Arista止血粉相近,且无细胞毒性,有望开发为体外快速止血材料。
文摘This study aims to develop collagen-based hemostatic materials. The sheet-shaped collagen sponge was manufactured by freeze-vacuum drying the aqueous solution of collagen (Col) and heat-denatured collagen (Col’) at a composition ratio of 2/1. The both sides or one side of sponge was treated with ultraviolet (UV) irradiation for 15 minutes to introduce intermolecular crosslinks between collagen molecules. The elution behavior of collagen sponge was investigated by immersing the sponge in water for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge showed very slow elution properties. On the other hand, the single-sided UV-irradiated sponge showed initially fast elution and subsequent very slow elution properties. Such initially fast elution of collagen molecules from the surface without UV-irradiation allows an adhesion of collagen sponge to the wound surface and results in hemostatic effect. In addition, the water absorption and retention properties of sponge were investigated by placing the hydrated sponge on a mesh for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge absorbed 81 times more water than own weight and showed a value of 45 times even after 7 days. The single-sided UV-irradiated sponge absorbed 80 times more water than own weight and showed a value of 39 times even after 7 days. The sponge with high water absorption and retention properties allows a wound healing effect because such sponge can absorb large amounts of blood plasma and exudates containing various cell growth factors. The double-sided UV-irradiated sponge is a good candidate for the wound dressing. On the other hand, the single-sided UV-irradiated sponge is a good candidate for the hemostatic material.
