Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract...Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.展开更多
基金supported by the Ministry of Research Technology and Higher Education of Indonesia 2016
文摘Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.
