<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels ...<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.展开更多
目的探讨原发性肾病综合征(PNS)患儿外周血单个核细胞(PBMC)上糖皮质激素受体β(GRβ)及热休克蛋白90(HSP90)与糖皮质激素治疗效应之间的关系。方法以30例PNS患儿作为观察组,根据随访结果分成激素耐药型肾病综合征(SRNS)和激素敏感型肾...目的探讨原发性肾病综合征(PNS)患儿外周血单个核细胞(PBMC)上糖皮质激素受体β(GRβ)及热休克蛋白90(HSP90)与糖皮质激素治疗效应之间的关系。方法以30例PNS患儿作为观察组,根据随访结果分成激素耐药型肾病综合征(SRNS)和激素敏感型肾病综合征(SSNS)2组,各15例。10例健康儿童作为正常对照组。应用RT-PCR检测了各组PBMC上GRβmRNA及HSP90mRNA的表达;并分析以上指标与肾脏病理及相关临床实验室指标的关系。结果 (1)SSNS组患儿PBMC上GRβmRNA、HSP90mRNA的表达均高于对照组(均P<0.05)。(2)SRNS组患儿PBMC上GRβmRNA、HSP90mRNA的表达均高于SSNS组(均P<0.01)。(3)SRNS组患儿PBMC上GRβmRNA的表达与肾脏病理分级成等级正相关(r=0.496,P<0.05);与24 h尿蛋白定量(24 h UTP)成等级正相关(r=0.458,P<0.05)。(4)SSNS组患儿PBMC上GRβmRNA的表达与24 h UTP无相关性(P>0.05)。结论 GRβ及HSP90可能参与了SRNS的发生与发展。GRβ可以作为监测PNS患儿病情以及预后的指标之一。展开更多
Background Left ventricular hypertrophy （LVH） induced by systemic hypertension represents a maladaptive response to the increased overload. However antihypertensive treatments are not always useful to regress or pre...Background Left ventricular hypertrophy （LVH） induced by systemic hypertension represents a maladaptive response to the increased overload. However antihypertensive treatments are not always useful to regress or prevent LVH. Thoroughly understanding the mechanisms will help to find new therapeutic targets that prevent or reverse left ventricular hypertrophy. A number of regulators and molecular signaling pathways have been shown to be involved in the hypertrophic process, such as angiotensin II, heat shock proteins 90, microRNAs, TRPC, mTOR, HDAC and PI3K/Akt signaling pathways. Therefore, other treatments, such as G1 cyclin antagonists, HSP90 inhibitor, Rho-kinase inhibitor, calcineurin blockers, CS866, statins, scutellarin, and aldosterone antagonist could prevent left ventricular hypertrophy.展开更多
文摘<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.
文摘目的探讨原发性肾病综合征(PNS)患儿外周血单个核细胞(PBMC)上糖皮质激素受体β(GRβ)及热休克蛋白90(HSP90)与糖皮质激素治疗效应之间的关系。方法以30例PNS患儿作为观察组,根据随访结果分成激素耐药型肾病综合征(SRNS)和激素敏感型肾病综合征(SSNS)2组,各15例。10例健康儿童作为正常对照组。应用RT-PCR检测了各组PBMC上GRβmRNA及HSP90mRNA的表达;并分析以上指标与肾脏病理及相关临床实验室指标的关系。结果 (1)SSNS组患儿PBMC上GRβmRNA、HSP90mRNA的表达均高于对照组(均P<0.05)。(2)SRNS组患儿PBMC上GRβmRNA、HSP90mRNA的表达均高于SSNS组(均P<0.01)。(3)SRNS组患儿PBMC上GRβmRNA的表达与肾脏病理分级成等级正相关(r=0.496,P<0.05);与24 h尿蛋白定量(24 h UTP)成等级正相关(r=0.458,P<0.05)。(4)SSNS组患儿PBMC上GRβmRNA的表达与24 h UTP无相关性(P>0.05)。结论 GRβ及HSP90可能参与了SRNS的发生与发展。GRβ可以作为监测PNS患儿病情以及预后的指标之一。
文摘Background Left ventricular hypertrophy （LVH） induced by systemic hypertension represents a maladaptive response to the increased overload. However antihypertensive treatments are not always useful to regress or prevent LVH. Thoroughly understanding the mechanisms will help to find new therapeutic targets that prevent or reverse left ventricular hypertrophy. A number of regulators and molecular signaling pathways have been shown to be involved in the hypertrophic process, such as angiotensin II, heat shock proteins 90, microRNAs, TRPC, mTOR, HDAC and PI3K/Akt signaling pathways. Therefore, other treatments, such as G1 cyclin antagonists, HSP90 inhibitor, Rho-kinase inhibitor, calcineurin blockers, CS866, statins, scutellarin, and aldosterone antagonist could prevent left ventricular hypertrophy.
