Objective： To investigate the anti-tumor immunity （HSP70-PC） from human lung cancer tissue. Methods in vitro of heat shock protein 70-peptide complexes HSP70-PC was purified from lung tumor tissues and correspondin...Objective： To investigate the anti-tumor immunity （HSP70-PC） from human lung cancer tissue. Methods in vitro of heat shock protein 70-peptide complexes HSP70-PC was purified from lung tumor tissues and corresponding non-tumor lung samples with the methods of ADP-affinity chromatography, DEAE ion-exchange chromatography and Western-blot. The activation and proliferation of PBMC induced by different HSP70-PC and tumor cytotoxic reactivity to A549 cells in vitro were measured by the MTT cell proliferation assay. Results： The purified HSP70-PC had a very high purity found by SDS-PAGE and Western-blot. Human lymphocytes were sensitized efficiently by HSP70 preparation purified from lung cancer tissues and a definite cytotoxicity to A-549 cells was observed. There was significant difference with HSP70-PC purified from lung cancer, compared with the control group （P〈0.001）. Conclusion： High purity of HSP70-PC could be achieved from tumor tissues in this study. HSP70-PC purified from human tumor tissues can induce anti-tumor immunity in vitro mainly implemented by eliciting CTL immunity.展开更多
本研究采用细胞冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,在体外构建热休克蛋白70（heat shock protein 70,HSP70）抗原肽复合物（HSP70-peptids complex,HSP70-PC）并观察该复合物对BABL/C小鼠脾细胞的激活与增殖作用及增殖后脾细胞对...本研究采用细胞冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,在体外构建热休克蛋白70（heat shock protein 70,HSP70）抗原肽复合物（HSP70-peptids complex,HSP70-PC）并观察该复合物对BABL/C小鼠脾细胞的激活与增殖作用及增殖后脾细胞对4T1细胞的杀伤作用;并进行体内实验观察HSP70-PC对BALB/C小鼠的免疫保护作用.目的在于为乳腺癌的免疫治疗探索一条新途径.展开更多
基金a grant from the Natural Science Foundation of Guangdong Province, China (No. 04010022).
文摘Objective： To investigate the anti-tumor immunity （HSP70-PC） from human lung cancer tissue. Methods in vitro of heat shock protein 70-peptide complexes HSP70-PC was purified from lung tumor tissues and corresponding non-tumor lung samples with the methods of ADP-affinity chromatography, DEAE ion-exchange chromatography and Western-blot. The activation and proliferation of PBMC induced by different HSP70-PC and tumor cytotoxic reactivity to A549 cells in vitro were measured by the MTT cell proliferation assay. Results： The purified HSP70-PC had a very high purity found by SDS-PAGE and Western-blot. Human lymphocytes were sensitized efficiently by HSP70 preparation purified from lung cancer tissues and a definite cytotoxicity to A-549 cells was observed. There was significant difference with HSP70-PC purified from lung cancer, compared with the control group （P〈0.001）. Conclusion： High purity of HSP70-PC could be achieved from tumor tissues in this study. HSP70-PC purified from human tumor tissues can induce anti-tumor immunity in vitro mainly implemented by eliciting CTL immunity.
文摘本研究采用细胞冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,在体外构建热休克蛋白70（heat shock protein 70,HSP70）抗原肽复合物（HSP70-peptids complex,HSP70-PC）并观察该复合物对BABL/C小鼠脾细胞的激活与增殖作用及增殖后脾细胞对4T1细胞的杀伤作用;并进行体内实验观察HSP70-PC对BALB/C小鼠的免疫保护作用.目的在于为乳腺癌的免疫治疗探索一条新途径.
