目的综合运用多维联用技术,建立日本救心丹HPLC-UV/ELSD和HPLC-UV/MS3指纹图谱研究方法,初步阐明其化学物质基础。方法A ltim a C18色谱柱(250 mm×4.6 mm ID,5μm);流动相为乙腈-0.2%甲酸水体系;梯度洗脱;流速1.0 mL.m in-1;检测波...目的综合运用多维联用技术,建立日本救心丹HPLC-UV/ELSD和HPLC-UV/MS3指纹图谱研究方法,初步阐明其化学物质基础。方法A ltim a C18色谱柱(250 mm×4.6 mm ID,5μm);流动相为乙腈-0.2%甲酸水体系;梯度洗脱;流速1.0 mL.m in-1;检测波长286 nm。ELSD条件为漂移管温度110℃,N2流速2.5 L.m in-1。采用电喷雾离子阱质谱分析,正、负离子三级扫描。结果建立了日本救心丹HPLC-UV/ELSD多维指纹图谱,同时通过MS定性鉴别出21种成分,并推测可能存在去氧苷胆酸及其异构体。方法稳定且重现性好。结论该研究为分析汉方中药复杂体系提供了一种有效、可靠的模式。展开更多
A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of...A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.展开更多
基金National Key Technology R&D Program"New Drug Innovation" of China (Grant No. 2012ZX09304-005,2012ZX09301002-002-002)
文摘A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.
