目的:基于肾脏尿酸转运环节,筛选及评价中药降尿酸活性成分,为阐释中药降尿酸物质基础提供理论和技术支撑。方法:以不同灌注压力进行大鼠离体肾脏灌流,通过肾小球滤过率、尿流率、葡萄糖重吸收率、钠离子重吸收率、钾离子重吸收率等评...目的:基于肾脏尿酸转运环节,筛选及评价中药降尿酸活性成分,为阐释中药降尿酸物质基础提供理论和技术支撑。方法:以不同灌注压力进行大鼠离体肾脏灌流,通过肾小球滤过率、尿流率、葡萄糖重吸收率、钠离子重吸收率、钾离子重吸收率等评价肾功能,建立离体肾脏模型;设置尿酸组、菊苣酸组和丙磺舒组进行离体肾脏灌流,观察肾脏尿酸排泄情况,以菊苣酸示例应用离体肾脏灌流模型;设置对照组、尿酸组、苯溴马隆组、丙磺舒组及菊苣酸组,通过蛋白免疫印迹法检测高尿酸状态下HKC细胞GLUT9蛋白表达变化,及高尿酸状态下菊苣酸干预后的表达变化,结合离体肾脏灌流技术评价降尿酸活性成分。结果:在120 mm Hg(1 mm Hg=0.133 kPa)灌注压力下离体肾功能良好;菊苣酸在20~40 min、40~60 min均能提高肾脏清除率,增加肾脏尿酸排泄分数;且菊苣酸能够显著抑制高尿酸状态下HKC细胞的GLUT9蛋白表达。结论:该研究基于肾脏尿酸转运环节,应用离体肾脏灌流技术结合体外HKC细胞筛选并评价中药降尿酸活性成分,筛选出菊苣酸可能是菊苣通过促进尿酸排泄发挥降尿酸作用的活性成分,为抗高尿酸血症药物的研发奠定实验基础,为中药的开发和临床应用提供实验依据,以更好服务于临床。展开更多
目的:采用低氧培养HKC细胞株,检测HKC STAT 1、α-SMA以及HIF-1α的动态表达,探讨JAK/STAT信号转导途径在低氧诱导的肾小管上皮上皮-间质转化中的作用。方法:将对数生长期HKC随机分为常规培养组(A组),常规+Flu培养组(B组),低氧培养组(C...目的:采用低氧培养HKC细胞株,检测HKC STAT 1、α-SMA以及HIF-1α的动态表达,探讨JAK/STAT信号转导途径在低氧诱导的肾小管上皮上皮-间质转化中的作用。方法:将对数生长期HKC随机分为常规培养组(A组),常规+Flu培养组(B组),低氧培养组(C组),低氧+Flu培养组(D组)4组。C组和D组采用三气培养箱低氧培养。分别在实验第1、3、6d观察细胞形态改变情况,免疫细胞化学检测HIF-1α、α-SMA以及STAT1表达。结果 :与A、B组比较,C、D组细胞生长缓慢,随着低氧培养时间的延长,细胞形态变细长,呈梭形。HIF-1α在A、B组表达极低,C、D组表达显著增强(P<0.05),各时间点差异无统计学意义。从第3 d开始C组STAT1、α-SMA表达显著高于A组(P<0.05)。与C组相比,D组各时间点STAT1表达显著降低(P<0.05),α-SMA从第3 d开始表达明显降低(P<0.05)。结论:低氧可能通过JAK/STAT1信号通路诱导肾小管上皮细胞发生表型转化,从而促进肾间质纤维化的发展。展开更多
We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stron...We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stronghold for underground music in the Fragrant Harbor since 2016.Founded by Gavin Wong,the mind behind essential Hong Kong label Absurd Trax,and Davy Law,who runs another excellent Hong Kong-based label,Neoncity Records,HKCR has become one of the most important music outlets in the Asia Pacific region.We spoke to Wong about the station.展开更多
We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stron...We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stronghold for underground music in the Fragrant Harbor since 2016.Founded by Gavin Wong,the mind behind essential Hong Kong label Absurd Trax,and Davy Law,who runs another excellent Hong Kong-based label,Neoncity Records,HKCR has become one of the most important music outlets in the Asia Pacific region.We spoke to Wong about the station.展开更多
文摘目的:基于肾脏尿酸转运环节,筛选及评价中药降尿酸活性成分,为阐释中药降尿酸物质基础提供理论和技术支撑。方法:以不同灌注压力进行大鼠离体肾脏灌流,通过肾小球滤过率、尿流率、葡萄糖重吸收率、钠离子重吸收率、钾离子重吸收率等评价肾功能,建立离体肾脏模型;设置尿酸组、菊苣酸组和丙磺舒组进行离体肾脏灌流,观察肾脏尿酸排泄情况,以菊苣酸示例应用离体肾脏灌流模型;设置对照组、尿酸组、苯溴马隆组、丙磺舒组及菊苣酸组,通过蛋白免疫印迹法检测高尿酸状态下HKC细胞GLUT9蛋白表达变化,及高尿酸状态下菊苣酸干预后的表达变化,结合离体肾脏灌流技术评价降尿酸活性成分。结果:在120 mm Hg(1 mm Hg=0.133 kPa)灌注压力下离体肾功能良好;菊苣酸在20~40 min、40~60 min均能提高肾脏清除率,增加肾脏尿酸排泄分数;且菊苣酸能够显著抑制高尿酸状态下HKC细胞的GLUT9蛋白表达。结论:该研究基于肾脏尿酸转运环节,应用离体肾脏灌流技术结合体外HKC细胞筛选并评价中药降尿酸活性成分,筛选出菊苣酸可能是菊苣通过促进尿酸排泄发挥降尿酸作用的活性成分,为抗高尿酸血症药物的研发奠定实验基础,为中药的开发和临床应用提供实验依据,以更好服务于临床。
文摘We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stronghold for underground music in the Fragrant Harbor since 2016.Founded by Gavin Wong,the mind behind essential Hong Kong label Absurd Trax,and Davy Law,who runs another excellent Hong Kong-based label,Neoncity Records,HKCR has become one of the most important music outlets in the Asia Pacific region.We spoke to Wong about the station.
文摘We’ve been covering online radio more and more recently,as a number of new stations crop up around Greater China.One of the first to get off the ground was Hong Kong Community Radio(HKCR),which has been a vital stronghold for underground music in the Fragrant Harbor since 2016.Founded by Gavin Wong,the mind behind essential Hong Kong label Absurd Trax,and Davy Law,who runs another excellent Hong Kong-based label,Neoncity Records,HKCR has become one of the most important music outlets in the Asia Pacific region.We spoke to Wong about the station.
