目的:利用TCGA数据库探讨生长停滞和DNA损伤诱导基因β(growth arrest and DNA damage-inducible geneβ,GADD45B)的基因表达与卵巢浆液性囊腺癌临床特征的相关性及其在卵巢浆液性囊腺癌预后分析中的价值。方法:下载TCGA数据库中604例...目的:利用TCGA数据库探讨生长停滞和DNA损伤诱导基因β(growth arrest and DNA damage-inducible geneβ,GADD45B)的基因表达与卵巢浆液性囊腺癌临床特征的相关性及其在卵巢浆液性囊腺癌预后分析中的价值。方法:下载TCGA数据库中604例卵巢浆液性囊腺癌患者的临床信息及307例卵巢浆液性囊腺癌患者的RNA测序数据,利用卡方检验和t检验分析GADD45B表达与卵巢浆液性囊腺癌临床病理特征的相关性,利用Kaplan-Meier法分析GADD45B表达与卵巢浆液性囊腺癌总生存时间的相关性,利用单因素和多因素COX回归分析GADD45B是否可以作为卵巢浆液性囊腺癌预后分析的独立分子标志物,利用qRT-PCR检测GADD45B在多药耐药卵巢癌细胞及对照卵巢癌细胞中的表达。结果:GADD45B表达与卵巢浆液性囊腺癌的国际妇产科联合会(International Federation of Gynecology and Obstetrics,FIGO)分期、肿瘤大小、肿瘤在卵巢的浸润情况、初次化学药物治疗效果及生存情况均显著相关,且与卵巢浆液性囊腺癌的总生存时间显著负相关;多因素COX回归分析示:只有年龄和初次化疗效果可以作为卵巢浆液性囊腺癌的独立分子标记,同时qRT-PCR发现GADD45B在多药耐药卵巢癌细胞中显著升高。结论:GADD45B在耐药卵巢癌细胞中显著升高,其高表达与卵巢浆液性囊腺癌预后不良及化疗耐药明显相关,可作为分析卵巢浆液性囊腺癌预后的有效分子标志物。展开更多
Polygonum cuspidatum is used as a traditional medicinal herb for the therapy of various diseases including several types of cancers. In the present study, we focused on addressing the anti-cancer activity and molecula...Polygonum cuspidatum is used as a traditional medicinal herb for the therapy of various diseases including several types of cancers. In the present study, we focused on addressing the anti-cancer activity and molecular mechanism of methanol extract of Polygonum cuspidatum (MEPC) in HSC-2 human oral cancer cells. The effect of MEPC on oral cancer cells was estimated by 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay, 4’-6-diamidino-2-phenylindole (DAPI) staining and Western blot analysis. MEPC inhibited the cell viability and induced apoptosis through the induction of death receptor (DR) 5. MEPC also increased the expression of C/EBP homologous protein/growth arrest and the DNA damage-inducible gene 153 (CHOP), a transcription factor induced by ER stress. Thus, we concluded that the induction of CHOP leading to DR5 up-regulation is required for the anti-cancer activity of MEPC in HSC-2 cells and MEPC may be a promising drug candidate for oral cancer.展开更多
文摘目的:利用TCGA数据库探讨生长停滞和DNA损伤诱导基因β(growth arrest and DNA damage-inducible geneβ,GADD45B)的基因表达与卵巢浆液性囊腺癌临床特征的相关性及其在卵巢浆液性囊腺癌预后分析中的价值。方法:下载TCGA数据库中604例卵巢浆液性囊腺癌患者的临床信息及307例卵巢浆液性囊腺癌患者的RNA测序数据,利用卡方检验和t检验分析GADD45B表达与卵巢浆液性囊腺癌临床病理特征的相关性,利用Kaplan-Meier法分析GADD45B表达与卵巢浆液性囊腺癌总生存时间的相关性,利用单因素和多因素COX回归分析GADD45B是否可以作为卵巢浆液性囊腺癌预后分析的独立分子标志物,利用qRT-PCR检测GADD45B在多药耐药卵巢癌细胞及对照卵巢癌细胞中的表达。结果:GADD45B表达与卵巢浆液性囊腺癌的国际妇产科联合会(International Federation of Gynecology and Obstetrics,FIGO)分期、肿瘤大小、肿瘤在卵巢的浸润情况、初次化学药物治疗效果及生存情况均显著相关,且与卵巢浆液性囊腺癌的总生存时间显著负相关;多因素COX回归分析示:只有年龄和初次化疗效果可以作为卵巢浆液性囊腺癌的独立分子标记,同时qRT-PCR发现GADD45B在多药耐药卵巢癌细胞中显著升高。结论:GADD45B在耐药卵巢癌细胞中显著升高,其高表达与卵巢浆液性囊腺癌预后不良及化疗耐药明显相关,可作为分析卵巢浆液性囊腺癌预后的有效分子标志物。
文摘Polygonum cuspidatum is used as a traditional medicinal herb for the therapy of various diseases including several types of cancers. In the present study, we focused on addressing the anti-cancer activity and molecular mechanism of methanol extract of Polygonum cuspidatum (MEPC) in HSC-2 human oral cancer cells. The effect of MEPC on oral cancer cells was estimated by 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay, 4’-6-diamidino-2-phenylindole (DAPI) staining and Western blot analysis. MEPC inhibited the cell viability and induced apoptosis through the induction of death receptor (DR) 5. MEPC also increased the expression of C/EBP homologous protein/growth arrest and the DNA damage-inducible gene 153 (CHOP), a transcription factor induced by ER stress. Thus, we concluded that the induction of CHOP leading to DR5 up-regulation is required for the anti-cancer activity of MEPC in HSC-2 cells and MEPC may be a promising drug candidate for oral cancer.
