A glycopolymer bearing glucose residues was tethered onto the surface of polypropylene microporous membrane by UV-induced graft polymerization ofα-allyl glucoside.Concanavalin A (Con A),a glucose recognizing lectin,c...A glycopolymer bearing glucose residues was tethered onto the surface of polypropylene microporous membrane by UV-induced graft polymerization ofα-allyl glucoside.Concanavalin A (Con A),a glucose recognizing lectin,could be specifically adsorbed to the membrane surface.On the other hand,the membrane surface showed no recognition ability to another lectin peanut agglutinin.Moreover,the recognition complex between the glycosylated membrane surface and Con A could be inhibited by glucose and mannose solution.T...展开更多
基金the National Natural Science Foundation of China (No.20474054)the National Natural Science Foundation of China for Distinguished Young Scholars (No.50625309).
文摘A glycopolymer bearing glucose residues was tethered onto the surface of polypropylene microporous membrane by UV-induced graft polymerization ofα-allyl glucoside.Concanavalin A (Con A),a glucose recognizing lectin,could be specifically adsorbed to the membrane surface.On the other hand,the membrane surface showed no recognition ability to another lectin peanut agglutinin.Moreover,the recognition complex between the glycosylated membrane surface and Con A could be inhibited by glucose and mannose solution.T...
文摘为了改善乳清蛋白可食膜的机械性能,采用干法糖基化改性乳清蛋白,制备乳清蛋白(WPC)-海藻酸钠(SA)共价复合物及其共价复合物膜。分析反应时间、乳化体系的p H值、底物配比(海藻酸钠/乳清蛋白)、相对湿度对WPC-SA共价复合物膜拉伸强度和断裂伸长率的影响;在单因素试验的基础上,采用Box-Behnken设计,对WPC-SA共价复合物膜的拉伸强度进行响应面分析。结果表明,WPC-SA共价复合物膜的最优工艺条件为反应时间60 h,乳化体系p H 8,底物配比3∶1(质量比)。经试验验证,此条件下的拉伸强度为(678.76±0.38)g,与预测值接近。采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳技术证明WPC和SA发生了共价结合反应;采用傅里叶红外光谱分析也表明乳清蛋白以共价键的结合形式接入了海藻酸钠分子。
