Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangl...Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangles caused by hyperphosphorylation of tau. An in vivo model of Alzheimer＇s disease was developed by injecting okadaic acid（2 μL） and exogenous BDNF（2 μL） into the hippocampi of adult male Wister rats. Spatial learning and memory abilities were assessed using the Morris water maze. The expression levels of protein phosphatase 2 A（PP2 A）, PP2 Ac-Yp307, p-tau（Thr231）, and p-tau（Ser396/404） were detected by western blot assay. The expression levels of BDNF, TrkB, and synaptophysin mRNA were measured by quantitative real-time polymerase chain reaction. Our results indicated that BDNF expression was suppressed in the hippocampus of OA-treated rats, which resulted in learning and memory deficits. Intra-hippocampal injection of BDNF attenuated this OA-induced cognitive impairment. Finally, our findings indicated an involvement of the PI3 K/GSK-3β/AKT pathway in the mechanism of BDNF in regulating cognitive function. These results indicate that BDNF has beneficial effect on Alzheimer＇s disease, and highlight the potential of BDNF as a drug target for treatment of Alzheimer＇s disease.展开更多
目的:通过观察丹蛭降糖胶囊对2型糖尿病患者大血管Wnt/β-链蛋白(Wnt/β-catenin)、糖原合成酶激酶-3(GSK-3β)通路表达,以及对颈动脉内膜中层厚度(IMT)和斑块面积的影响,评价丹蛭降糖胶囊对2型糖尿病患者大血管病变的保护作用。方法:将...目的:通过观察丹蛭降糖胶囊对2型糖尿病患者大血管Wnt/β-链蛋白(Wnt/β-catenin)、糖原合成酶激酶-3(GSK-3β)通路表达,以及对颈动脉内膜中层厚度(IMT)和斑块面积的影响,评价丹蛭降糖胶囊对2型糖尿病患者大血管病变的保护作用。方法:将102例2型糖尿病患者随机分为对照组和治疗组各51人。所有患者给予基础药物(常规口服降糖药物)治疗,治疗组采用基础药物+丹蛭降糖胶囊,疗程均为16周。结果:16周后治疗组总有效率84.31%,优于对照组的64.71%,差异有统计学意义(P<0.05);两组治疗前后比较中医证候积分、FPG、2 h PG、Hb A1c、TC、TG、LDL、HDL、颈动脉IMT及斑块面积水平都有所改善(P<0.05或P<0.01);组间比较,治疗组中医证候积分、2 h PG、Hb A1c、TG及LDL水平下降,具有统计学差异(P<0.05或P<0.01);治疗组IMT改善情况优于对照组(P<0.05),但斑块面积改善情况无统计学意义(P>0.05)。治疗后,治疗组血清β-catenin水平较对照组显著降低(P<0.01),血清GSK-3β水平比对照组降低,差异有统计学意义(P<0.05)。结论:丹蛭降糖胶囊可以显著缓解2型糖尿病患者临床症状,并在一定程度上改善或逆转颈动脉硬化进程。展开更多
文摘Decreased expression of brain-derived neurotrophic factor（BDNF） plays an important role in the pathogenesis of Alzheimer＇s disease, and a typical pathological change in Alzheimer＇s disease is neurofibrillary tangles caused by hyperphosphorylation of tau. An in vivo model of Alzheimer＇s disease was developed by injecting okadaic acid（2 μL） and exogenous BDNF（2 μL） into the hippocampi of adult male Wister rats. Spatial learning and memory abilities were assessed using the Morris water maze. The expression levels of protein phosphatase 2 A（PP2 A）, PP2 Ac-Yp307, p-tau（Thr231）, and p-tau（Ser396/404） were detected by western blot assay. The expression levels of BDNF, TrkB, and synaptophysin mRNA were measured by quantitative real-time polymerase chain reaction. Our results indicated that BDNF expression was suppressed in the hippocampus of OA-treated rats, which resulted in learning and memory deficits. Intra-hippocampal injection of BDNF attenuated this OA-induced cognitive impairment. Finally, our findings indicated an involvement of the PI3 K/GSK-3β/AKT pathway in the mechanism of BDNF in regulating cognitive function. These results indicate that BDNF has beneficial effect on Alzheimer＇s disease, and highlight the potential of BDNF as a drug target for treatment of Alzheimer＇s disease.
文摘目的:通过观察丹蛭降糖胶囊对2型糖尿病患者大血管Wnt/β-链蛋白(Wnt/β-catenin)、糖原合成酶激酶-3(GSK-3β)通路表达,以及对颈动脉内膜中层厚度(IMT)和斑块面积的影响,评价丹蛭降糖胶囊对2型糖尿病患者大血管病变的保护作用。方法:将102例2型糖尿病患者随机分为对照组和治疗组各51人。所有患者给予基础药物(常规口服降糖药物)治疗,治疗组采用基础药物+丹蛭降糖胶囊,疗程均为16周。结果:16周后治疗组总有效率84.31%,优于对照组的64.71%,差异有统计学意义(P<0.05);两组治疗前后比较中医证候积分、FPG、2 h PG、Hb A1c、TC、TG、LDL、HDL、颈动脉IMT及斑块面积水平都有所改善(P<0.05或P<0.01);组间比较,治疗组中医证候积分、2 h PG、Hb A1c、TG及LDL水平下降,具有统计学差异(P<0.05或P<0.01);治疗组IMT改善情况优于对照组(P<0.05),但斑块面积改善情况无统计学意义(P>0.05)。治疗后,治疗组血清β-catenin水平较对照组显著降低(P<0.01),血清GSK-3β水平比对照组降低,差异有统计学意义(P<0.05)。结论:丹蛭降糖胶囊可以显著缓解2型糖尿病患者临床症状,并在一定程度上改善或逆转颈动脉硬化进程。
