[ Objective] To clone and analyze the intedeukin-2 （IL.2） gene in Gaoyou ducks. [ Method] With total RNA isolated from peripheral lym- phocytes of Gaoyou ducks as templates, one pair of primers was designed accordin...[ Objective] To clone and analyze the intedeukin-2 （IL.2） gene in Gaoyou ducks. [ Method] With total RNA isolated from peripheral lym- phocytes of Gaoyou ducks as templates, one pair of primers was designed according to the known IL-2 gene sequence in ducks. The IL-2 DNA was amplified by the RT-PCR and inserted into pGEM-T-easy vector. Then the positive recombinant plasmid was sequenced. [ Result] The amplified target fragment was 433 bp with the correct size as theoretically expected. The open reading frame （ORF） was 423 bp encoding a precursor protein with 140 amino acids which consisted of a signal peptide composed of 21 amino acids and a mature peptide composed of 119 amino acids. The pro- tein was approximately 13.66 kDa and contained one N-glycosylation site. The sequence of IL-2 gene of Gaoyou duck had 99.8% nucleotide homol- ogy to that of Anas platyrhynchos duck, Gushi duck and Shaoxing duck, respectively. It also had 99.3% nucleotide homology to that of Guangzhou duck. [ Conclusion] The coding region of IL-2 gene of ducks is highly conserved.展开更多
基金supported by the Natural Science Funds of Jiangsu Province(BK2009701)Education Reform Research Funds of Yangzhou University in 2009
文摘[ Objective] To clone and analyze the intedeukin-2 （IL.2） gene in Gaoyou ducks. [ Method] With total RNA isolated from peripheral lym- phocytes of Gaoyou ducks as templates, one pair of primers was designed according to the known IL-2 gene sequence in ducks. The IL-2 DNA was amplified by the RT-PCR and inserted into pGEM-T-easy vector. Then the positive recombinant plasmid was sequenced. [ Result] The amplified target fragment was 433 bp with the correct size as theoretically expected. The open reading frame （ORF） was 423 bp encoding a precursor protein with 140 amino acids which consisted of a signal peptide composed of 21 amino acids and a mature peptide composed of 119 amino acids. The pro- tein was approximately 13.66 kDa and contained one N-glycosylation site. The sequence of IL-2 gene of Gaoyou duck had 99.8% nucleotide homol- ogy to that of Anas platyrhynchos duck, Gushi duck and Shaoxing duck, respectively. It also had 99.3% nucleotide homology to that of Guangzhou duck. [ Conclusion] The coding region of IL-2 gene of ducks is highly conserved.
