OBJECTIVE:To explore the mechanism of Maxingxiongting mixture(MXXTM)on pulmonary hypertension in a rat model established by intraperitoneal injection of monocrotaline solution,smoking and forced swimming.METHODS:A tot...OBJECTIVE:To explore the mechanism of Maxingxiongting mixture(MXXTM)on pulmonary hypertension in a rat model established by intraperitoneal injection of monocrotaline solution,smoking and forced swimming.METHODS:A total of 30 male Sprague-Dawley rats were randomly divided into five groups:control group,model group,high-dose of MXXTM group(HM),low-dose of MXXTM group(LM),and fasudil group.The mean pulmonary artery pressure(m PAP)was measured by using a miniature catheter.Lung tissue and right ventricular tissue sections were stained with hematoxylin-eosin.The right ventricle(RV)and left ventricle+septum(LV+S)were weighted.RV/(LV+S)was calculated to reflect the degree of right ventricular hypertrophy.Rho/Rho-kinase signaling pathway key proteins(Rho A,ROCKⅠand ROCKⅡ)in rat right ventricular tissue were measured by Western blot analysis.The levels of serum hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)and the levels of plasma renin activity(PRA),angiotensinⅡ(ANG-Ⅱ),aldosterone(ALD)in rat anticoagulated plasma were all measured by enzyme-linked immunosorbent assay.RESULTS:Compared with the control group,the m PAP and RV/(LV+S)in the model group were significantly increased.Administration of fasudil resulted in a significant decrease of m PAP and RV/(LV+S).In the HM group and LM group,m PAP and RV/(LV+S)were significantly lower than the model group.Compared with the control group,the contents of HIF-1α,VEGF,PRA,ANG-Ⅱand ALD in the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the contents of HIF-1α,VEGF,PRA,ANG-II and ALD.Compared with the control group,the expression of Rho A,ROCKⅠand ROCKⅡin the right ventricle of the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the expression of Rho A and RockⅡproteins.Our results indicated that high-dose of MXXTM had similar effects on reducing pulmonary artery pressure and improving right ventricul展开更多
: The full-length cDNA of the wheat (Triticum aestivum L.) root hair defective 3 gene (RHD3) has been cloned from the salt-tolerant hybrid wheat variety Shanrong No. 3 (Za3) using the mRNA differential display and 5’...: The full-length cDNA of the wheat (Triticum aestivum L.) root hair defective 3 gene (RHD3) has been cloned from the salt-tolerant hybrid wheat variety Shanrong No. 3 (Za3) using the mRNA differential display and 5’rapid amplification of cDNA ends (RACE) methods. Analysis of the amino acid sequence deduced from the wheat RHD3, gene shows that two conservative GTP-binding motifs, namely GXXXXGKS and DXXG, in eukaryotes also exist at the N-terminal of wheat RHD3. In addition, an 18 amino acid residue transmembrane domain, namely FYLAVMFVVFLVGKAIWV, exists at positions 701—718 of the C-terminal of the deduced protein of wheat RHD3 obtained, but this domain is absent in another three proteins aligned, including rice RHD3, Arabidopsis RHD3, and yeast homologue SEY1. Northern blot revealed that transcription of the wheat RHD3, gene is down-regulated in both the salt-tolerant line and in JN177 under saline stress. A possible stress-responsive mechanism for this gene is discussed.展开更多
T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and prolif...T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation.展开更多
Signal transduction across cell membranes is an important subject of the current studies on biomembranes. Hormonally regulated adenylate cyclase signalling system is composed of three distinct types of plasma-membrane...Signal transduction across cell membranes is an important subject of the current studies on biomembranes. Hormonally regulated adenylate cyclase signalling system is composed of three distinct types of plasma-membrane associated proteins: the receptor, adenylate cyclase (AC) and stimulatory GTP-binding protein (Gs) which me-展开更多
目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组...目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组(癫发作后1、3、7、14、30、60 d),荧光定量聚合酶链反应、Western blotting法和免疫组织化学染色检测各观察时间点大鼠海马组织GIT1表达变化。结果癫组大鼠GIT1 m RNA自癫持续状态后急性期第1和3天即升高(P=0.012,0.002),至潜伏期第7和14天持续升高(P=0.003,0.001),至慢性期第30和60天达峰值水平(均P=0.000);GIT1蛋白自急性期即升高,慢性期持续升高,但与对照组相比,差异未达统计学意义(均P>0.05),至慢性期达峰值水平(均P=0.000)。至慢性期第30天时,癫组大鼠海马CA1区、齿状回和海马旁皮质GIT1蛋白表达水平均高于对照组(P=0.000)。结论癫大鼠海马组织GIT1表达上调,可能通过调节细胞骨架动态重组而影响兴奋性神经网络,从而参与癫的发生。展开更多
目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌...目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌病组（其中增殖期内膜18例，分泌期内膜14例）；同期因宫颈病变或卵巢肿瘤而行子宫切除的患者29例为对照组（其中增殖期内膜12例，分泌期内膜17例）。对两组患者子宫内膜一肌层交界区平滑肌细胞进行分离及原代培养。应用实时荧光定量PCR技术和蛋白印迹法，检测两组及子宫腺肌病组不同程度痛经患者子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCK I mRNA及其蛋白的表达水平。结果（1）子宫腺肌病组子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCKImRNA表达水平无明显周期性变化，增殖期表达水平（分别为1．41±0．16、1．05±0．15）稍高于分泌期（分别为1．17±0．25、0．98±0．10），分别比较，差异均无统计学意义（P〉0．05）；对照组RhoA和ROCKImRNA表达水平存在周期性变化，增殖期表达水平（分别为0．93±0．10、1．00±0．18）高于分泌期（0．48±0．03、0．55±0．05），分别比较，差异均有统计学意义（P〈0．05）；子宫腺肌病组增殖期和分泌期RhoA和ROCKImRNA的表达水平均高于对照组，分别比较，差异均有统计学意义（P〈0．01）。（2）子宫腺肌病组与对照组RhoA及ROCKImRNA、蛋白表达水平均呈正相关关系（r=0．67，P〈0．01；r=0．48，P〈0．01）。（3）RhoA和ROCKImRNA、蛋白表达水平在子宫腺肌病组不同程度痛经患者中依次表现为：重度痛经（1．66±0．19、1．32±0．11）〉中度痛经（1．28±0．12、1．09±0．08）〉轻度痛经（0．93±0．09、0．81±0．06），两两分别比较，差异均有统计学意义（P〈0．05）。结论子宫腺肌病患者子展开更多
基金Supported by State Administration of Traditional Chinese Medicine"Hundred Million"Talent Project of Inheritance and Innovation of Traditional Chinese Medicine National Old Chinese Medicine Experts Academic Experience Inheritance Project(Issued by the Ministry of Education of Traditional Chinese Medicine 2017 No.29)Technological Innovation Project"Baitang"Physical Examination and Analysis of the Significance of PrescriptionSichuang Provincial Science and Technology Department(No.2018RZ0126)。
文摘OBJECTIVE:To explore the mechanism of Maxingxiongting mixture(MXXTM)on pulmonary hypertension in a rat model established by intraperitoneal injection of monocrotaline solution,smoking and forced swimming.METHODS:A total of 30 male Sprague-Dawley rats were randomly divided into five groups:control group,model group,high-dose of MXXTM group(HM),low-dose of MXXTM group(LM),and fasudil group.The mean pulmonary artery pressure(m PAP)was measured by using a miniature catheter.Lung tissue and right ventricular tissue sections were stained with hematoxylin-eosin.The right ventricle(RV)and left ventricle+septum(LV+S)were weighted.RV/(LV+S)was calculated to reflect the degree of right ventricular hypertrophy.Rho/Rho-kinase signaling pathway key proteins(Rho A,ROCKⅠand ROCKⅡ)in rat right ventricular tissue were measured by Western blot analysis.The levels of serum hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)and the levels of plasma renin activity(PRA),angiotensinⅡ(ANG-Ⅱ),aldosterone(ALD)in rat anticoagulated plasma were all measured by enzyme-linked immunosorbent assay.RESULTS:Compared with the control group,the m PAP and RV/(LV+S)in the model group were significantly increased.Administration of fasudil resulted in a significant decrease of m PAP and RV/(LV+S).In the HM group and LM group,m PAP and RV/(LV+S)were significantly lower than the model group.Compared with the control group,the contents of HIF-1α,VEGF,PRA,ANG-Ⅱand ALD in the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the contents of HIF-1α,VEGF,PRA,ANG-II and ALD.Compared with the control group,the expression of Rho A,ROCKⅠand ROCKⅡin the right ventricle of the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the expression of Rho A and RockⅡproteins.Our results indicated that high-dose of MXXTM had similar effects on reducing pulmonary artery pressure and improving right ventricul
文摘: The full-length cDNA of the wheat (Triticum aestivum L.) root hair defective 3 gene (RHD3) has been cloned from the salt-tolerant hybrid wheat variety Shanrong No. 3 (Za3) using the mRNA differential display and 5’rapid amplification of cDNA ends (RACE) methods. Analysis of the amino acid sequence deduced from the wheat RHD3, gene shows that two conservative GTP-binding motifs, namely GXXXXGKS and DXXG, in eukaryotes also exist at the N-terminal of wheat RHD3. In addition, an 18 amino acid residue transmembrane domain, namely FYLAVMFVVFLVGKAIWV, exists at positions 701—718 of the C-terminal of the deduced protein of wheat RHD3 obtained, but this domain is absent in another three proteins aligned, including rice RHD3, Arabidopsis RHD3, and yeast homologue SEY1. Northern blot revealed that transcription of the wheat RHD3, gene is down-regulated in both the salt-tolerant line and in JN177 under saline stress. A possible stress-responsive mechanism for this gene is discussed.
文摘T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation.
文摘Signal transduction across cell membranes is an important subject of the current studies on biomembranes. Hormonally regulated adenylate cyclase signalling system is composed of three distinct types of plasma-membrane associated proteins: the receptor, adenylate cyclase (AC) and stimulatory GTP-binding protein (Gs) which me-
文摘目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组(癫发作后1、3、7、14、30、60 d),荧光定量聚合酶链反应、Western blotting法和免疫组织化学染色检测各观察时间点大鼠海马组织GIT1表达变化。结果癫组大鼠GIT1 m RNA自癫持续状态后急性期第1和3天即升高(P=0.012,0.002),至潜伏期第7和14天持续升高(P=0.003,0.001),至慢性期第30和60天达峰值水平(均P=0.000);GIT1蛋白自急性期即升高,慢性期持续升高,但与对照组相比,差异未达统计学意义(均P>0.05),至慢性期达峰值水平(均P=0.000)。至慢性期第30天时,癫组大鼠海马CA1区、齿状回和海马旁皮质GIT1蛋白表达水平均高于对照组(P=0.000)。结论癫大鼠海马组织GIT1表达上调,可能通过调节细胞骨架动态重组而影响兴奋性神经网络,从而参与癫的发生。
文摘目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌病组（其中增殖期内膜18例，分泌期内膜14例）；同期因宫颈病变或卵巢肿瘤而行子宫切除的患者29例为对照组（其中增殖期内膜12例，分泌期内膜17例）。对两组患者子宫内膜一肌层交界区平滑肌细胞进行分离及原代培养。应用实时荧光定量PCR技术和蛋白印迹法，检测两组及子宫腺肌病组不同程度痛经患者子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCK I mRNA及其蛋白的表达水平。结果（1）子宫腺肌病组子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCKImRNA表达水平无明显周期性变化，增殖期表达水平（分别为1．41±0．16、1．05±0．15）稍高于分泌期（分别为1．17±0．25、0．98±0．10），分别比较，差异均无统计学意义（P〉0．05）；对照组RhoA和ROCKImRNA表达水平存在周期性变化，增殖期表达水平（分别为0．93±0．10、1．00±0．18）高于分泌期（0．48±0．03、0．55±0．05），分别比较，差异均有统计学意义（P〈0．05）；子宫腺肌病组增殖期和分泌期RhoA和ROCKImRNA的表达水平均高于对照组，分别比较，差异均有统计学意义（P〈0．01）。（2）子宫腺肌病组与对照组RhoA及ROCKImRNA、蛋白表达水平均呈正相关关系（r=0．67，P〈0．01；r=0．48，P〈0．01）。（3）RhoA和ROCKImRNA、蛋白表达水平在子宫腺肌病组不同程度痛经患者中依次表现为：重度痛经（1．66±0．19、1．32±0．11）〉中度痛经（1．28±0．12、1．09±0．08）〉轻度痛经（0．93±0．09、0．81±0．06），两两分别比较，差异均有统计学意义（P〈0．05）。结论子宫腺肌病患者子
