目的研究FLT3基因表达水平及内部串联重复（internal tandem duplication，ITD）突变与急性髓系白血病（AML）的关系及临床意义。方法采用实时定量RT-PCR技术检测129例AML患者治疗前后共152份骨髓标本单个核细胞FLT3mRNA，应用RT-PCR技...目的研究FLT3基因表达水平及内部串联重复（internal tandem duplication，ITD）突变与急性髓系白血病（AML）的关系及临床意义。方法采用实时定量RT-PCR技术检测129例AML患者治疗前后共152份骨髓标本单个核细胞FLT3mRNA，应用RT-PCR技术检测其中75例初发未治AML患者的FLT3／ITD突变。结果80例初发未治AML患者FLT3mRNA表达水平为0．1041（0～33．8736），10名正常人骨髓FLT3mRNA表达水平为0．0020（0．0006～0．0040），差异有统计学意义（P=0．001）。75例初发未治AML患者检出FLT3／ITD突变者11例，突变率14．7％，FLT3／ITD突变组的FLT3 mRNA表达水平为0．2200（0．0297～33．8736），与FLT3／ITD阴性组[0．0975（0～26．2200）]比较，差异无统计学意义（P=0．093）。在M3患者中，FLT3／ITD阳性者缓解率显著低于FLT3／ITD阴性者（P=0．015）。FLT3／ITD阴性的非M3患者中FLT3 mRNA高表达组（表达水平〉0．04）患者的化疗缓解率（37．5％）显著低于低表达组（缓解率100％）。追踪观察20例患者，完全缓解组的FLT3 mRNA表达水平迅速或缓慢下降至少1个对数级，未缓解组的FLT3 mRNA表达水平无明显变化。结论AML患者FLT3 mRNA的表达量及FLT3／ITD突变，可作为评价AML患者治疗效果、估计预后的指标，为微量残留病监测、观察AML患者病情变化提供有用工具。展开更多
The dendritic cell system contains conventional dendritic cells(DCs)and plasmacytoid pre-dendritic cells (pDCs).Both DCs and pDCs are bone marrow derived cells.Although the common functions of DCs are antigen-processi...The dendritic cell system contains conventional dendritic cells(DCs)and plasmacytoid pre-dendritic cells (pDCs).Both DCs and pDCs are bone marrow derived cells.Although the common functions of DCs are antigen-processing and T-lymphocyte activation,they differ in surface markers,migratory patterns,and cytokine output.These differences can determine the fate of the T cells they activate.Several subsets of mature DCs have been described in both mouse and human and the developmental processes of these specialized DC subsets have been studied extensively.The original concept that all DCs were of myeloid origin was questioned by several recent studies,which demonstrated that in addition to the DCs derived from myeloid precursors, some DCs could also be efficiently generated from lymphoid-restricted precursors.Moreover,it has been shown recently that both conventional DCs and pDCs can be generated by the Flt3 expressing hemopoietic pro- genitors regardless of their myeloid-or lymphoid-origin.These findings suggest an early developmental flexibility of precursors for DCs and pDCs.This review summarizes some recent observations on the deve- lopment of DC system in both human and mouse.Cellular & Molecular Immunology.2004;1(2):112-118.展开更多
文摘The dendritic cell system contains conventional dendritic cells(DCs)and plasmacytoid pre-dendritic cells (pDCs).Both DCs and pDCs are bone marrow derived cells.Although the common functions of DCs are antigen-processing and T-lymphocyte activation,they differ in surface markers,migratory patterns,and cytokine output.These differences can determine the fate of the T cells they activate.Several subsets of mature DCs have been described in both mouse and human and the developmental processes of these specialized DC subsets have been studied extensively.The original concept that all DCs were of myeloid origin was questioned by several recent studies,which demonstrated that in addition to the DCs derived from myeloid precursors, some DCs could also be efficiently generated from lymphoid-restricted precursors.Moreover,it has been shown recently that both conventional DCs and pDCs can be generated by the Flt3 expressing hemopoietic pro- genitors regardless of their myeloid-or lymphoid-origin.These findings suggest an early developmental flexibility of precursors for DCs and pDCs.This review summarizes some recent observations on the deve- lopment of DC system in both human and mouse.Cellular & Molecular Immunology.2004;1(2):112-118.
