目的优选黄芪多糖脂质体的制备工艺,并对其性能进行评价。方法采用薄膜分散超声法制备黄芪多糖脂质体,以包封率、多分散系数等为评价指标,采用单因素实验优化处方;并对其粒径、表面电位、稳定性及体外释药行为进行考察。结果制备的APS...目的优选黄芪多糖脂质体的制备工艺,并对其性能进行评价。方法采用薄膜分散超声法制备黄芪多糖脂质体,以包封率、多分散系数等为评价指标,采用单因素实验优化处方;并对其粒径、表面电位、稳定性及体外释药行为进行考察。结果制备的APS脂质体的平均粒径为334.1 nm,表面Zeta电位为-33.7 m V,药物的平均包封率为42.32%,体外15 h累积释药百分率为55.65%。结论制备的黄芪多糖脂质体包封率高,4℃下贮存较稳定,且具有良好的缓释作用。展开更多
目的采用薄膜分散-超声法制备磁性颗粒(bacterial magnetic particles,BMPs)脂质体,考察BMPs浓度、超声功率和超声时间等因素对BMPs磁性脂质体粒径的影响。方法薄膜分散-超声法制备BMPs脂质体,调节BMPs浓度、超声功率和超声时间等因素,...目的采用薄膜分散-超声法制备磁性颗粒(bacterial magnetic particles,BMPs)脂质体,考察BMPs浓度、超声功率和超声时间等因素对BMPs磁性脂质体粒径的影响。方法薄膜分散-超声法制备BMPs脂质体,调节BMPs浓度、超声功率和超声时间等因素,激光散射粒度仪测定磁性脂质体粒径。结果以薄膜分散-超声法制备的BMPs脂质体,BMPs浓度在(20～60)μg/mL时,磁性脂质体粒径基本稳定,平均94.9 nm;BMPs浓度在(60～100)μg/mL时,磁性脂质体粒径随着BMPs浓度增加而有增大的趋势。超声功率的增加或超声有效时间增大时,磁性脂质体的平均粒径有减小趋势,当超声功率为300 W、有效超声时间为100 s时,粒径出现最小值;但其后都存在转折,随着超声功率的再增加或超声有效时间再增大时,平均粒径反而出现增大现象。结论薄膜分散-超声法制备BMPs脂质体,通过控制BMPs浓度、超声功率和超声时间等因素,可以对磁性脂质体粒径进行调节。展开更多
In the present work, we aimed to optimize the preparation technology of dimethyl curcumin niosomes, improve its solubility and assess its stability. The novel anti-androgen dimethyl curcumin niosomes were prepared by ...In the present work, we aimed to optimize the preparation technology of dimethyl curcumin niosomes, improve its solubility and assess its stability. The novel anti-androgen dimethyl curcumin niosomes were prepared by thin-film dispersion-ultrasonic method, and the prescription composition and preparation process were optimized by single-factor investigation method. Certainly, the solubility and quality evaluation of dimethyl curcumin niosomes were also investigated. The average particle size of prepared dimethyl curcumin niosomes was （310.3＋0.9） nm. The highest encapsulation rate was 88.1%± 1.7%, and the drug-loading amount was 4.03%±1.05%. Moreover, the leakage rate was below 2% within 45 d. Collectively, all these findings indicated that the niosomes, as a vector, could significantly improve the solubility and stability of dimethyl curcumin, offering a theoretical basis for dimethyl curcumin as an anticancer drug in medicine application.展开更多
目的采用Box-Behnken响应面法优化白藜芦醇(resveratrol)-辛酸癸酸聚乙二醇甘油酯(Labrasol)/泊洛沙姆407(P407)混合胶束(Res-Labrasol/P407-MM)的处方工艺,并考察其体外特征。方法HPLC法测定白藜芦醇含量。薄膜分散-探头超声法制备Res-...目的采用Box-Behnken响应面法优化白藜芦醇(resveratrol)-辛酸癸酸聚乙二醇甘油酯(Labrasol)/泊洛沙姆407(P407)混合胶束(Res-Labrasol/P407-MM)的处方工艺,并考察其体外特征。方法HPLC法测定白藜芦醇含量。薄膜分散-探头超声法制备Res-Labrasol/P407-MM。在单因素实验结果的基础上,以投药量、水化体积和水化温度为考察因素,胶束样品的质量浓度和药物沉降率为评价指标,采用Box-Behnken响应面法优化处方,并对最优处方制备的胶束进行外观形态、粒径和体外释放行为评价。结果最佳处方工艺为白藜芦醇投药量28 mg,水化体积9 m L,水化温度40℃,在此条件下测得白藜芦醇平均载药量为(11.62±0.20)%、平均包封率为(93.96±1.83)%、胶束平均粒径为(69.00±1.58)nm,平均Zeta电位为(-21.25±0.18)m V。样品质量浓度和沉降率的实测值与预测值偏差较小,RSD<5.5%。结论Box-Behnken响应面法预测性良好,可以用于优化Res-Labrasol/P407-MM的处方工艺。制备的混合胶束包封率高,粒径小,分布均匀,缓释效果明显。展开更多
文摘目的优选黄芪多糖脂质体的制备工艺,并对其性能进行评价。方法采用薄膜分散超声法制备黄芪多糖脂质体,以包封率、多分散系数等为评价指标,采用单因素实验优化处方;并对其粒径、表面电位、稳定性及体外释药行为进行考察。结果制备的APS脂质体的平均粒径为334.1 nm,表面Zeta电位为-33.7 m V,药物的平均包封率为42.32%,体外15 h累积释药百分率为55.65%。结论制备的黄芪多糖脂质体包封率高,4℃下贮存较稳定,且具有良好的缓释作用。
基金The National Natural Science Foundation of China(Grant No.31271002)Jiangsu Provincial Department of Education Funded Projects(Grant No.14KJB350001)
文摘In the present work, we aimed to optimize the preparation technology of dimethyl curcumin niosomes, improve its solubility and assess its stability. The novel anti-androgen dimethyl curcumin niosomes were prepared by thin-film dispersion-ultrasonic method, and the prescription composition and preparation process were optimized by single-factor investigation method. Certainly, the solubility and quality evaluation of dimethyl curcumin niosomes were also investigated. The average particle size of prepared dimethyl curcumin niosomes was （310.3＋0.9） nm. The highest encapsulation rate was 88.1%± 1.7%, and the drug-loading amount was 4.03%±1.05%. Moreover, the leakage rate was below 2% within 45 d. Collectively, all these findings indicated that the niosomes, as a vector, could significantly improve the solubility and stability of dimethyl curcumin, offering a theoretical basis for dimethyl curcumin as an anticancer drug in medicine application.
文摘目的采用Box-Behnken响应面法优化白藜芦醇(resveratrol)-辛酸癸酸聚乙二醇甘油酯(Labrasol)/泊洛沙姆407(P407)混合胶束(Res-Labrasol/P407-MM)的处方工艺,并考察其体外特征。方法HPLC法测定白藜芦醇含量。薄膜分散-探头超声法制备Res-Labrasol/P407-MM。在单因素实验结果的基础上,以投药量、水化体积和水化温度为考察因素,胶束样品的质量浓度和药物沉降率为评价指标,采用Box-Behnken响应面法优化处方,并对最优处方制备的胶束进行外观形态、粒径和体外释放行为评价。结果最佳处方工艺为白藜芦醇投药量28 mg,水化体积9 m L,水化温度40℃,在此条件下测得白藜芦醇平均载药量为(11.62±0.20)%、平均包封率为(93.96±1.83)%、胶束平均粒径为(69.00±1.58)nm,平均Zeta电位为(-21.25±0.18)m V。样品质量浓度和沉降率的实测值与预测值偏差较小,RSD<5.5%。结论Box-Behnken响应面法预测性良好,可以用于优化Res-Labrasol/P407-MM的处方工艺。制备的混合胶束包封率高,粒径小,分布均匀,缓释效果明显。
