Two new compounds,schinifolin Ⅰ and acetoxyschinifolin Ⅱ,along with five known compounds(auraptenⅢ,dictamnine Ⅳ,scoparone Ⅴ,skimmianine Ⅵ,and β-sitosterol Ⅶ)were isolated from the roots of Zanthoxylum schinifo...Two new compounds,schinifolin Ⅰ and acetoxyschinifolin Ⅱ,along with five known compounds(auraptenⅢ,dictamnine Ⅳ,scoparone Ⅴ,skimmianine Ⅵ,and β-sitosterol Ⅶ)were isolated from the roots of Zanthoxylum schinifolium Sieb.et Zucc.col- lected in Yixing County,Jiangsu Province.The structure determination was based upon spectroscopic analysis(UV,IR,MS,PMR,CMR,2D NMR).The structures of schinifolin Ⅰ and acetoxyschinifolin Ⅱ were elucidated as 7[(3′,7′-dimethyl-2′,6′-octadienyl)] oxy-8-methoxy-2H-1-benzopyran-2-one,and 7[(3′,7′-dimethyl-5′-acetoxy-2′, 6′-octadienyl)]oxy-8-methoxy-2H-1-benzopyran-2-one,respectively. In the test of platelet aggregation caused by PAF,compounds Ⅰ,Ⅱ,Ⅲ and Ⅴ showed inhibitory activity.展开更多
目的观察银杏内酯注射液、银杏内酯ABC以及银杏叶提取物注射液(EGb761)对家兔血小板聚集功能、超微结构及PF-4和β-TG含量的影响比较。方法将日本大耳兔分为对照(生理盐水)组、EGb761(1.02 m L/kg)组、银杏内酯ABC(5.13 mg/kg)组、银杏...目的观察银杏内酯注射液、银杏内酯ABC以及银杏叶提取物注射液(EGb761)对家兔血小板聚集功能、超微结构及PF-4和β-TG含量的影响比较。方法将日本大耳兔分为对照(生理盐水)组、EGb761(1.02 m L/kg)组、银杏内酯ABC(5.13 mg/kg)组、银杏内酯注射液(1.02 m L/kg,以萜内酯计5.1 mg/kg)组、白果内酯(5.13 mg/kg)组,分别iv相应药物1周。给药结束后,家兔心脏取血,观察PAF诱导下血小板聚集率;电镜观察血小板超微结构;试剂盒法观察PAF诱导下血清中血小板因子-4(PF-4)和β-血小板球蛋白(β-TG)含量。结果与对照组比较,EGb761组、银杏内酯ABC组、银杏内酯注射液组的血小板聚集率均显著降低(P<0.05、0.01),白果内酯组无显著变化;血小板聚集抑制率:银杏内酯注射液(43.76%)>银杏内酯ABC(35.3%)>EGb761(26.52%)>白果内酯(5.48%)。与对照组比较,EGb761、银杏内酯ABC、银杏内酯注射液均能减少聚集型血小板数量,使树突型血小板突起变少变短。与对照组比较,银杏内酯注射液和银杏内酯ABC均使PF-4和β-TG表达显著降低(P<0.01),EGb761仅显著降低β-TG表达(P<0.05)。结论银杏内酯注射液通过PAF途径发挥抗血小板聚集作用,其作用强于银杏内酯ABC,可能是白果内酯发挥了协同增效作用。展开更多
文摘Two new compounds,schinifolin Ⅰ and acetoxyschinifolin Ⅱ,along with five known compounds(auraptenⅢ,dictamnine Ⅳ,scoparone Ⅴ,skimmianine Ⅵ,and β-sitosterol Ⅶ)were isolated from the roots of Zanthoxylum schinifolium Sieb.et Zucc.col- lected in Yixing County,Jiangsu Province.The structure determination was based upon spectroscopic analysis(UV,IR,MS,PMR,CMR,2D NMR).The structures of schinifolin Ⅰ and acetoxyschinifolin Ⅱ were elucidated as 7[(3′,7′-dimethyl-2′,6′-octadienyl)] oxy-8-methoxy-2H-1-benzopyran-2-one,and 7[(3′,7′-dimethyl-5′-acetoxy-2′, 6′-octadienyl)]oxy-8-methoxy-2H-1-benzopyran-2-one,respectively. In the test of platelet aggregation caused by PAF,compounds Ⅰ,Ⅱ,Ⅲ and Ⅴ showed inhibitory activity.
文摘目的观察银杏内酯注射液、银杏内酯ABC以及银杏叶提取物注射液(EGb761)对家兔血小板聚集功能、超微结构及PF-4和β-TG含量的影响比较。方法将日本大耳兔分为对照(生理盐水)组、EGb761(1.02 m L/kg)组、银杏内酯ABC(5.13 mg/kg)组、银杏内酯注射液(1.02 m L/kg,以萜内酯计5.1 mg/kg)组、白果内酯(5.13 mg/kg)组,分别iv相应药物1周。给药结束后,家兔心脏取血,观察PAF诱导下血小板聚集率;电镜观察血小板超微结构;试剂盒法观察PAF诱导下血清中血小板因子-4(PF-4)和β-血小板球蛋白(β-TG)含量。结果与对照组比较,EGb761组、银杏内酯ABC组、银杏内酯注射液组的血小板聚集率均显著降低(P<0.05、0.01),白果内酯组无显著变化;血小板聚集抑制率:银杏内酯注射液(43.76%)>银杏内酯ABC(35.3%)>EGb761(26.52%)>白果内酯(5.48%)。与对照组比较,EGb761、银杏内酯ABC、银杏内酯注射液均能减少聚集型血小板数量,使树突型血小板突起变少变短。与对照组比较,银杏内酯注射液和银杏内酯ABC均使PF-4和β-TG表达显著降低(P<0.01),EGb761仅显著降低β-TG表达(P<0.05)。结论银杏内酯注射液通过PAF途径发挥抗血小板聚集作用,其作用强于银杏内酯ABC,可能是白果内酯发挥了协同增效作用。
