[ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were ...[ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were cultured conventionally and randomly separated into two groups. The experimental group was trea- ted with 5 μg/rnl Rbl for 20 min, while control group was added with the same amount of medium. After cell lysis, the whole-cell protein was extracted. Two-di- mensional electrophoresis (2-DE) was used to separate the extracts. Differential expression of proteome between the two groups was analyzed by using ImageMaster 2D Platinum v5.0 software and the two protein spots expressed differently were selected for differential identification with MALDI-TOF-MS. [ Result] Based on the matching and comparative analysis of the protein spots, 418 protein spots were detected in experimental group, including 226 protein spots with differently expres- sive levels; according to the mass spectrometry, the two ginsenoside Rbl-related and differentially-expressed protein spots were identified as cytochrome P-450 and phosducin-like protein, and both of them were phosphorylated proteins. [ Conclusion ] This study showed that the functions of those identified proteins were in- volved in signal transduction, suggesting that the effect of ginsenoside Rbl on expressive proteome of rat neurons might be related to the corresponding signal trans- duction networks.展开更多
基金Supported by the Fund of Sichuan Provincial Department of Education( 2006C010)
文摘[ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformat- ies and MS peptide fingerprinting. [ Method ] Rat neurons were cultured conventionally and randomly separated into two groups. The experimental group was trea- ted with 5 μg/rnl Rbl for 20 min, while control group was added with the same amount of medium. After cell lysis, the whole-cell protein was extracted. Two-di- mensional electrophoresis (2-DE) was used to separate the extracts. Differential expression of proteome between the two groups was analyzed by using ImageMaster 2D Platinum v5.0 software and the two protein spots expressed differently were selected for differential identification with MALDI-TOF-MS. [ Result] Based on the matching and comparative analysis of the protein spots, 418 protein spots were detected in experimental group, including 226 protein spots with differently expres- sive levels; according to the mass spectrometry, the two ginsenoside Rbl-related and differentially-expressed protein spots were identified as cytochrome P-450 and phosducin-like protein, and both of them were phosphorylated proteins. [ Conclusion ] This study showed that the functions of those identified proteins were in- volved in signal transduction, suggesting that the effect of ginsenoside Rbl on expressive proteome of rat neurons might be related to the corresponding signal trans- duction networks.