Human interferon-α cDNA has been successfully introduced into tobacco plant cells. And some plants producing active IFN-α have been obtained. By blunt end ligation of DNA, human IFN-α cDNA was inserted into the Bam...Human interferon-α cDNA has been successfully introduced into tobacco plant cells. And some plants producing active IFN-α have been obtained. By blunt end ligation of DNA, human IFN-α cDNA was inserted into the BamHI site between the promotor of transcripton 1 and the tailing signal of transcripton 7 in the plant expression plasmid pAP2304, and so a recombinant plasmid pIG3031 was constructed, and the recombinant plasmid was introduced into the T-region of the Ti plasmid vector pGV3850. By means of agrobacterium infection to the leaf disc of general tobacco plant 1551, some transformed plants resistant to kana-mycin were obtained. DNA Southern hybridization showed that the human IFN-α gene together with T-DNA was integrated into the genome of tobacco cells. The assay of neomycin phosphate transferase Ⅱ indicated that the ability of resistance to kanamycin in the transformed plants resulted from the expression of the NPT Ⅱ gene. The assay of the biological activity of IFN-α showed that active IFN-α could be produced from the transformed plants.展开更多
文摘Human interferon-α cDNA has been successfully introduced into tobacco plant cells. And some plants producing active IFN-α have been obtained. By blunt end ligation of DNA, human IFN-α cDNA was inserted into the BamHI site between the promotor of transcripton 1 and the tailing signal of transcripton 7 in the plant expression plasmid pAP2304, and so a recombinant plasmid pIG3031 was constructed, and the recombinant plasmid was introduced into the T-region of the Ti plasmid vector pGV3850. By means of agrobacterium infection to the leaf disc of general tobacco plant 1551, some transformed plants resistant to kana-mycin were obtained. DNA Southern hybridization showed that the human IFN-α gene together with T-DNA was integrated into the genome of tobacco cells. The assay of neomycin phosphate transferase Ⅱ indicated that the ability of resistance to kanamycin in the transformed plants resulted from the expression of the NPT Ⅱ gene. The assay of the biological activity of IFN-α showed that active IFN-α could be produced from the transformed plants.
