Serological tests for antibodies specific for Epstein-Barr virus(EBV) antigens are frequently used to define infection status and for the differential diagnosis of other pathogens responsible for mononucleosis syndrom...Serological tests for antibodies specific for Epstein-Barr virus(EBV) antigens are frequently used to define infection status and for the differential diagnosis of other pathogens responsible for mononucleosis syndrome. Using only three parameters [viral capsid antigen(VCA) Ig G, VCA Ig M and EBV nuclear antigen(EBNA)-1 Ig G],it is normally possible to distinguish acute from past infection: the presence of VCA Ig M and VCA Ig G without EBNA-1 Ig G indicates acute infection, whereas the presence of VCA Ig G and EBNA-1 Ig G without VCA Ig M is typical of past infection. However, serological findings may sometimes be difficult to interpret as VCA Ig G can be present without VCA Ig M or EBNA-1 Ig G in cases of acute or past infection, or all the three parameters may be detected simultaneously in the case of recent infection or during the course of reactivation. A profile of isolated EBNA-1 Ig G may also create some doubts. In order to interpret these patterns correctly, it is necessary to determine Ig G avidity, identify anti-EBV Ig G and Ig M antibodies by immunoblotting, and look for heterophile antibodies, anti-EA(D) antibodies or viral genome using molecular biology methods. These tests make it possible to define the status of the infection and solve any problems that may arise in routine laboratory practice.展开更多
BACKGROUND Post-transplant lymphoproliferative disorder(PTLD) is a fatal complication of solid organ transplantation or allogenic hematopoietic stem cell transplantation that is associated with immunosuppressive thera...BACKGROUND Post-transplant lymphoproliferative disorder(PTLD) is a fatal complication of solid organ transplantation or allogenic hematopoietic stem cell transplantation that is associated with immunosuppressive therapy. Potential manifestations are diverse, ranging from reactive lymphoid hyperplasia to high-grade lymphoma.PTLD is usually of B-cell origin and associated with Epstein-Barr virus(EBV)infection. Herein, we describe a case of PTLD involving the peritoneal omentum.There has been only case of PTLD as a diffuse large B-cell lymphoma(DLBCL) in the peritoneum.CASE SUMMARY The patient was a 62-year-old man who had been receiving immunosuppressive therapy with tacrolimus since undergoing a liver transplant 15 years prior. He reported that he had experienced abdominal discomfort and anorexia 1 month prior to the current admission. Abdominal pelvic computed tomography(CT)revealed peritoneal and omental mass-like lesions without bowel obstruction.Ultrasonography-guided biopsy was performed, and he was histologically diagnosed with EBV-negative DLBCL. Positron emission tomography(PET)-CT depicted peritoneum and omentum involvement only, without any lymphadenopathy or organ masses, including in the gastrointestinal tract. Six cycles of chemotherapy with a "R-CHOP" regimen(rituximab-cyclophosphamide, doxorubicin, vincristine, prednisolone) were administered,and PET-CT performed thereafter indicated complete remission.CONCLUSION This is the first report of isolated peritoneal lymphomatosis defined as PTLD in a liver transplant recipient.展开更多
目的探讨EB病毒(EBV)感染相关性肾损伤患儿辅助性T细胞17(Th17)/调节性T细胞(Treg)相关细胞因子及百令胶囊联合抗病毒药物的疗效。方法选取2020年6月-2022年6月南阳市第一人民医院收治的EBV感染相关性肾损伤患儿140例,按随机数字表法分...目的探讨EB病毒(EBV)感染相关性肾损伤患儿辅助性T细胞17(Th17)/调节性T细胞(Treg)相关细胞因子及百令胶囊联合抗病毒药物的疗效。方法选取2020年6月-2022年6月南阳市第一人民医院收治的EBV感染相关性肾损伤患儿140例,按随机数字表法分为常规组70例和研究组70例;常规组予以更昔洛韦抗病毒治疗,研究组予以百令胶囊联合更昔洛韦抗病毒治疗,均治疗14 d;比较两组患儿临床疗效、治疗前后肾功能[尿素氮(BUN)、血肌酐(SCr)、24 h尿蛋白定量(24 h Upro)]、T淋巴细胞亚群(CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)、CD_(4)^(+)/CD_(8)^(+))水平、Th17/Treg细胞水平及Th17/Treg相关细胞因子[白细胞介素(IL)-17、转化生长因子-β1(TGF-β1)、IL-10]水平。结果研究组疗效优于常规组(P<0.05);治疗后,研究组BUN、SCr、24 h Upro、CD_(8)^(+)、Th17、Th17/Treg及IL-17低于常规组(P<0.05),CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水、Treg及TGF-β1、IL-10水平高于常规组(P<0.05)。结论百令胶囊联合抗病毒药物治疗EBV感染相关性肾损伤患儿疗效显著,可明显改善患儿肾功能,纠正T淋巴细胞亚群及Th17/Treg细胞失衡,增强患儿免疫功能。展开更多
文摘Serological tests for antibodies specific for Epstein-Barr virus(EBV) antigens are frequently used to define infection status and for the differential diagnosis of other pathogens responsible for mononucleosis syndrome. Using only three parameters [viral capsid antigen(VCA) Ig G, VCA Ig M and EBV nuclear antigen(EBNA)-1 Ig G],it is normally possible to distinguish acute from past infection: the presence of VCA Ig M and VCA Ig G without EBNA-1 Ig G indicates acute infection, whereas the presence of VCA Ig G and EBNA-1 Ig G without VCA Ig M is typical of past infection. However, serological findings may sometimes be difficult to interpret as VCA Ig G can be present without VCA Ig M or EBNA-1 Ig G in cases of acute or past infection, or all the three parameters may be detected simultaneously in the case of recent infection or during the course of reactivation. A profile of isolated EBNA-1 Ig G may also create some doubts. In order to interpret these patterns correctly, it is necessary to determine Ig G avidity, identify anti-EBV Ig G and Ig M antibodies by immunoblotting, and look for heterophile antibodies, anti-EA(D) antibodies or viral genome using molecular biology methods. These tests make it possible to define the status of the infection and solve any problems that may arise in routine laboratory practice.
基金Supported by the National Research Foundation of Korea,No.NRF-2017R1C1B5076793
文摘BACKGROUND Post-transplant lymphoproliferative disorder(PTLD) is a fatal complication of solid organ transplantation or allogenic hematopoietic stem cell transplantation that is associated with immunosuppressive therapy. Potential manifestations are diverse, ranging from reactive lymphoid hyperplasia to high-grade lymphoma.PTLD is usually of B-cell origin and associated with Epstein-Barr virus(EBV)infection. Herein, we describe a case of PTLD involving the peritoneal omentum.There has been only case of PTLD as a diffuse large B-cell lymphoma(DLBCL) in the peritoneum.CASE SUMMARY The patient was a 62-year-old man who had been receiving immunosuppressive therapy with tacrolimus since undergoing a liver transplant 15 years prior. He reported that he had experienced abdominal discomfort and anorexia 1 month prior to the current admission. Abdominal pelvic computed tomography(CT)revealed peritoneal and omental mass-like lesions without bowel obstruction.Ultrasonography-guided biopsy was performed, and he was histologically diagnosed with EBV-negative DLBCL. Positron emission tomography(PET)-CT depicted peritoneum and omentum involvement only, without any lymphadenopathy or organ masses, including in the gastrointestinal tract. Six cycles of chemotherapy with a "R-CHOP" regimen(rituximab-cyclophosphamide, doxorubicin, vincristine, prednisolone) were administered,and PET-CT performed thereafter indicated complete remission.CONCLUSION This is the first report of isolated peritoneal lymphomatosis defined as PTLD in a liver transplant recipient.
文摘目的探讨EB病毒(EBV)感染相关性肾损伤患儿辅助性T细胞17(Th17)/调节性T细胞(Treg)相关细胞因子及百令胶囊联合抗病毒药物的疗效。方法选取2020年6月-2022年6月南阳市第一人民医院收治的EBV感染相关性肾损伤患儿140例,按随机数字表法分为常规组70例和研究组70例;常规组予以更昔洛韦抗病毒治疗,研究组予以百令胶囊联合更昔洛韦抗病毒治疗,均治疗14 d;比较两组患儿临床疗效、治疗前后肾功能[尿素氮(BUN)、血肌酐(SCr)、24 h尿蛋白定量(24 h Upro)]、T淋巴细胞亚群(CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)、CD_(4)^(+)/CD_(8)^(+))水平、Th17/Treg细胞水平及Th17/Treg相关细胞因子[白细胞介素(IL)-17、转化生长因子-β1(TGF-β1)、IL-10]水平。结果研究组疗效优于常规组(P<0.05);治疗后,研究组BUN、SCr、24 h Upro、CD_(8)^(+)、Th17、Th17/Treg及IL-17低于常规组(P<0.05),CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水、Treg及TGF-β1、IL-10水平高于常规组(P<0.05)。结论百令胶囊联合抗病毒药物治疗EBV感染相关性肾损伤患儿疗效显著,可明显改善患儿肾功能,纠正T淋巴细胞亚群及Th17/Treg细胞失衡,增强患儿免疫功能。
