The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent intera...The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.展开更多
Objective This study aimed to investigate the reasons behind the lower survival rates in male lung cancer patients than in female lung cancer patients.Methods Through various techniques,such as Argonaute immunoprecipi...Objective This study aimed to investigate the reasons behind the lower survival rates in male lung cancer patients than in female lung cancer patients.Methods Through various techniques,such as Argonaute immunoprecipitation,luciferase assays,and ChIP,this study confirmed the positive effects of androgen receptor(AR)on lung cancer cell invasion across different in vitro cell lines and in vivo mouse models.Results The findings suggest that AR enhanced the invasion of lung cancer cells by modifying EPHB2 signals at the protein expression level,which in turn required changes in miRNA-23a-3p.Restoring miRNA-23a-3p could counteract the intensified invasion of lung cancer cells mediated by AR.Conclusion This study revealed that AR may facilitate the lung cancer matastasis by modulating miRNA-23a-3p/EPHB2 signaling and that targeting this signaling pathway could provide new approaches to inhibit lung cancer metastasis.展开更多
目的:观察清胰化积方(QYHJ)对不同EphB2表达人胰腺癌CFPAC-1裸鼠皮下移植瘤生长抑制作用差异,明确EphB2表达是否可以作为QYHJ干预有效性的预测靶点。方法:采用慢病毒介导RNAi技术建立不同EphB2表达状态人胰腺癌CFPAC-1细胞,建立裸鼠皮...目的:观察清胰化积方(QYHJ)对不同EphB2表达人胰腺癌CFPAC-1裸鼠皮下移植瘤生长抑制作用差异,明确EphB2表达是否可以作为QYHJ干预有效性的预测靶点。方法:采用慢病毒介导RNAi技术建立不同EphB2表达状态人胰腺癌CFPAC-1细胞,建立裸鼠皮下移植瘤模型并以QYHJ干预4周完成相关检测。结果:QYHJ干预4周后,CFPAC-1、CFPAC-1 Con RNAi和CFPAC-1 EphB2 RNAi细胞抑瘤率分别为31.40%、31.33%和18.36%。干预后CFPAC-1 Con RNi细胞G0/G1期比例由70.77%上升至78.47%,S期细胞由22.70%降至15.17%,S期比例与0.9%氯化钠溶液(NS)组比较差异有统计学意义(P<0.05)。干预后CFPAC-1 Con RNAi凋亡细胞比例由4.77%增加至20.50%,与NS组比较差异有统计学意义(P<0.05)。结论:EphB2相对高表达的CFPAC-1细胞对QYHJ干预更为敏感,其通过降低S期细胞比例进而抑制肿瘤细胞增殖并促进凋亡而发挥抑瘤作用;QYHJ对EphB2低表达细胞生长抑制不明显;EphB2高表达可以作为是清胰化积方干预后有效性的预测指标之一。展开更多
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutes of China(PAPD)the Science and Technology Plan Project of Nantong of China,No.JC2020026(to JW)the National Science Research of Jiangsu Higher Education Institutions of China,No.19KJB310012(to RYY)。
文摘The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.
基金supported by the Zhongnanshan Medical Foundation of Guangdong Province(No.ZNSXS-20220072).
文摘Objective This study aimed to investigate the reasons behind the lower survival rates in male lung cancer patients than in female lung cancer patients.Methods Through various techniques,such as Argonaute immunoprecipitation,luciferase assays,and ChIP,this study confirmed the positive effects of androgen receptor(AR)on lung cancer cell invasion across different in vitro cell lines and in vivo mouse models.Results The findings suggest that AR enhanced the invasion of lung cancer cells by modifying EPHB2 signals at the protein expression level,which in turn required changes in miRNA-23a-3p.Restoring miRNA-23a-3p could counteract the intensified invasion of lung cancer cells mediated by AR.Conclusion This study revealed that AR may facilitate the lung cancer matastasis by modulating miRNA-23a-3p/EPHB2 signaling and that targeting this signaling pathway could provide new approaches to inhibit lung cancer metastasis.
文摘目的:观察清胰化积方(QYHJ)对不同EphB2表达人胰腺癌CFPAC-1裸鼠皮下移植瘤生长抑制作用差异,明确EphB2表达是否可以作为QYHJ干预有效性的预测靶点。方法:采用慢病毒介导RNAi技术建立不同EphB2表达状态人胰腺癌CFPAC-1细胞,建立裸鼠皮下移植瘤模型并以QYHJ干预4周完成相关检测。结果:QYHJ干预4周后,CFPAC-1、CFPAC-1 Con RNAi和CFPAC-1 EphB2 RNAi细胞抑瘤率分别为31.40%、31.33%和18.36%。干预后CFPAC-1 Con RNi细胞G0/G1期比例由70.77%上升至78.47%,S期细胞由22.70%降至15.17%,S期比例与0.9%氯化钠溶液(NS)组比较差异有统计学意义(P<0.05)。干预后CFPAC-1 Con RNAi凋亡细胞比例由4.77%增加至20.50%,与NS组比较差异有统计学意义(P<0.05)。结论:EphB2相对高表达的CFPAC-1细胞对QYHJ干预更为敏感,其通过降低S期细胞比例进而抑制肿瘤细胞增殖并促进凋亡而发挥抑瘤作用;QYHJ对EphB2低表达细胞生长抑制不明显;EphB2高表达可以作为是清胰化积方干预后有效性的预测指标之一。
