Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in mos...Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in most of NPC tumors.Exploring EBERs as a prognostic marker for NPC might further be informative about the biology and the progression of the disease.The aim of this study was to analyze the role of EBV latency in the clinical management of nasopharyngeal carcinoma （NPC）,by detecting EBERs.Methods RNA in situ hybridization （ISH） for detecting EBERs was carried out on 908 NPC tumor tissues.Overall survival （OS） curves were analyzed with the Kaplan-Meier method and the Cox proportional-hazards regression models.Results The median follow-up time was 70 months （1-120 months）.Eight hundred and sixteen （89.9％） from a total of 908 consecutive NPC cases were found to be EBV-EBER positive.EBER-ISH staining revealed nuclear localization in NPC cells.In the Kaplan-Meier analysis for OS,high EBER expression levels in NPC patients were statistically significant positive prognostic factors for survival （log-rank,P=0.022）,especially in adults aged 17-40 years （P=0.023） and in those with advanced stage disease （log-rank,P=0.002）.Cox proportional-hazards regression model analysis showed that the EBER expression level was an independent risk factor for OS （hazard ratio 0.724,P=0.005）.Conclusions EBERs were frequently detected in NPC tumor tissues,and high-level EBER expression correlated with good prognosis in NPC patients,especially in adult patients and in those with advanced stage disease.EBER may serve as a potential prognostic predictor in NPC.展开更多
急性乙型肝炎发病危险因素研究对于预防乙型肝炎具有重要意义，但既往研究存在非新发病例、调查因素不全、病例标准不严谨等问题。福建省是乙型肝炎高流行地区，人群乙型肝炎病毒（hepatitis B virus ，HBV）感染率和乙型肝炎表面抗原（...急性乙型肝炎发病危险因素研究对于预防乙型肝炎具有重要意义，但既往研究存在非新发病例、调查因素不全、病例标准不严谨等问题。福建省是乙型肝炎高流行地区，人群乙型肝炎病毒（hepatitis B virus ，HBV）感染率和乙型肝炎表面抗原（hepatitis B surface antigen ，HBsAg）流行率远高于全国平均水平。本研究选取3个乙型肝炎高流行县（市）建立乙型肝炎监测系统，对新发乙型肝炎病例进行匹配病例-对照研究，以探讨急性乙型肝炎的影响因素。展开更多
文摘Background Epstein-Barr virus （EBV） infection is one of the most important factors of nasopharyngeal carcinoma （NPC）endemic areas.Transcription of EBV-encoded non-polyadenylated RNAs （EBERs） are presented in most of NPC tumors.Exploring EBERs as a prognostic marker for NPC might further be informative about the biology and the progression of the disease.The aim of this study was to analyze the role of EBV latency in the clinical management of nasopharyngeal carcinoma （NPC）,by detecting EBERs.Methods RNA in situ hybridization （ISH） for detecting EBERs was carried out on 908 NPC tumor tissues.Overall survival （OS） curves were analyzed with the Kaplan-Meier method and the Cox proportional-hazards regression models.Results The median follow-up time was 70 months （1-120 months）.Eight hundred and sixteen （89.9％） from a total of 908 consecutive NPC cases were found to be EBV-EBER positive.EBER-ISH staining revealed nuclear localization in NPC cells.In the Kaplan-Meier analysis for OS,high EBER expression levels in NPC patients were statistically significant positive prognostic factors for survival （log-rank,P=0.022）,especially in adults aged 17-40 years （P=0.023） and in those with advanced stage disease （log-rank,P=0.002）.Cox proportional-hazards regression model analysis showed that the EBER expression level was an independent risk factor for OS （hazard ratio 0.724,P=0.005）.Conclusions EBERs were frequently detected in NPC tumor tissues,and high-level EBER expression correlated with good prognosis in NPC patients,especially in adult patients and in those with advanced stage disease.EBER may serve as a potential prognostic predictor in NPC.
文摘急性乙型肝炎发病危险因素研究对于预防乙型肝炎具有重要意义，但既往研究存在非新发病例、调查因素不全、病例标准不严谨等问题。福建省是乙型肝炎高流行地区，人群乙型肝炎病毒（hepatitis B virus ，HBV）感染率和乙型肝炎表面抗原（hepatitis B surface antigen ，HBsAg）流行率远高于全国平均水平。本研究选取3个乙型肝炎高流行县（市）建立乙型肝炎监测系统，对新发乙型肝炎病例进行匹配病例-对照研究，以探讨急性乙型肝炎的影响因素。
