Objective To investigate whether pretreatment with repeated electroacupuncture (EA)at the Baihui acupoint could induce ischemic tolerance against transient focal cerebral ischemic injury in rats. Methods Thirty mal...Objective To investigate whether pretreatment with repeated electroacupuncture (EA)at the Baihui acupoint could induce ischemic tolerance against transient focal cerebral ischemic injury in rats. Methods Thirty male Sprague-Dawley (SD) rats were randomly divided into 3 groups (n=10 for each): the control group consisted of animals receiving no treatment, the isoflurane (ISO) group had animals that inhaled 1.5% isoflurane for 30 min a day for 5 days, and animals in the EA group received electroacupuncture at the Baihui acupoint for 30 min a day for 5 days under 1.5% isoflurane anesthesia. Twenty-four hours after the last treatment, the middle cerebral artery was occluded with No. 3 nylon monofilament for 120 min. The neurological outcomes were evaluated 24 h after reperfusion. The infarct volumes were then assessed using 2% triphenyltetrazolium chloride staining after the neurological outcome evaluation. Results The neurological deficit score (NDS) of the EA group was lower than that of the ISO group and the control group , P<0.05. The infarct volume of the EA group (38.3±25.4 mm 3) was significantly smaller than that of the control group (220.5±66.0 mm 3) and the ISO group (168.6±57.6 mm 3) 24 h after reperfusion. Conclusion Electroacupuncture at the Baihui acupoint 30 min a day for 5 days significantly reduces neurological injury induced by transient middle cerebral artery occlusion.展开更多
目的为了探讨针刺足阳明经穴对胃运动的调整作用是否与脑肠肽有关。方法 60只大鼠随机分为正常对照组、模型组、针剌四白组、天枢组、足三里组、非穴组,以乙醇灌胃造成大鼠胃粘膜损伤模型,气囊法测量胃运动频率和波幅的变化率,采用放免...目的为了探讨针刺足阳明经穴对胃运动的调整作用是否与脑肠肽有关。方法 60只大鼠随机分为正常对照组、模型组、针剌四白组、天枢组、足三里组、非穴组,以乙醇灌胃造成大鼠胃粘膜损伤模型,气囊法测量胃运动频率和波幅的变化率,采用放免分析法(RIA)检测大鼠胃窦及延髓内SP,MTT含量。结果模型组胃运动频率和波幅呈抑制状态,其胃运动频率和波幅的变化率(％)与对照组比较出现显著差异(-4.7±10.3vs-19.4±17.2.P<0.05;1.8±14.1 vs -51.6±29.0,P<0.01),针剌四白、天枢、足三里穴使胃运动恢复,其波幅(％)较模型组比较分别为-6.5±23.3,1.5±20.1,6.9±25.2vs-51.6±29.0,P<0.01)。针剌非穴组与对照组比较则末出现明显差异。与此同时胃窦、延髓内SP,MTL含量出现相应变化,模型组胃窦SP含量(pg·ML^(-1))低于对照组(41±11vs56±13,P<0.05),针剌天枢、足三里后胃窦SP含量较模型组明显升高(58±13和61±13vs41±11,P<0.05)。模型组延髓SP含量高于正常组,针剌组则均低于模型组。模型组胃窦、延髓内MTL含量(Pg·mL^(-1))均低于对照组,针剌足三里其延髓MTL含量明显高于模型组(59±10vs47±13,P<0.05).三穴比较针剌四白穴主要影响胃窦MTL含量,针刺天枢主要影响胃窦SP含量变化,针剌足三里穴既使胃窦SP升高又使延髓MTL含量升高。结论针剌足阳明经对胃运动的调整作用有脑肠肽参与,但上述三穴对胃运动的影响所涉及的脑肠肽不完全一致。展开更多
基金ThisstudywassupportedinpartbyagrantfromtheNationalNaturalScienceFoundationofChina (No 30 170 90 7)
文摘Objective To investigate whether pretreatment with repeated electroacupuncture (EA)at the Baihui acupoint could induce ischemic tolerance against transient focal cerebral ischemic injury in rats. Methods Thirty male Sprague-Dawley (SD) rats were randomly divided into 3 groups (n=10 for each): the control group consisted of animals receiving no treatment, the isoflurane (ISO) group had animals that inhaled 1.5% isoflurane for 30 min a day for 5 days, and animals in the EA group received electroacupuncture at the Baihui acupoint for 30 min a day for 5 days under 1.5% isoflurane anesthesia. Twenty-four hours after the last treatment, the middle cerebral artery was occluded with No. 3 nylon monofilament for 120 min. The neurological outcomes were evaluated 24 h after reperfusion. The infarct volumes were then assessed using 2% triphenyltetrazolium chloride staining after the neurological outcome evaluation. Results The neurological deficit score (NDS) of the EA group was lower than that of the ISO group and the control group , P<0.05. The infarct volume of the EA group (38.3±25.4 mm 3) was significantly smaller than that of the control group (220.5±66.0 mm 3) and the ISO group (168.6±57.6 mm 3) 24 h after reperfusion. Conclusion Electroacupuncture at the Baihui acupoint 30 min a day for 5 days significantly reduces neurological injury induced by transient middle cerebral artery occlusion.
文摘目的为了探讨针刺足阳明经穴对胃运动的调整作用是否与脑肠肽有关。方法 60只大鼠随机分为正常对照组、模型组、针剌四白组、天枢组、足三里组、非穴组,以乙醇灌胃造成大鼠胃粘膜损伤模型,气囊法测量胃运动频率和波幅的变化率,采用放免分析法(RIA)检测大鼠胃窦及延髓内SP,MTT含量。结果模型组胃运动频率和波幅呈抑制状态,其胃运动频率和波幅的变化率(％)与对照组比较出现显著差异(-4.7±10.3vs-19.4±17.2.P<0.05;1.8±14.1 vs -51.6±29.0,P<0.01),针剌四白、天枢、足三里穴使胃运动恢复,其波幅(％)较模型组比较分别为-6.5±23.3,1.5±20.1,6.9±25.2vs-51.6±29.0,P<0.01)。针剌非穴组与对照组比较则末出现明显差异。与此同时胃窦、延髓内SP,MTL含量出现相应变化,模型组胃窦SP含量(pg·ML^(-1))低于对照组(41±11vs56±13,P<0.05),针剌天枢、足三里后胃窦SP含量较模型组明显升高(58±13和61±13vs41±11,P<0.05)。模型组延髓SP含量高于正常组,针剌组则均低于模型组。模型组胃窦、延髓内MTL含量(Pg·mL^(-1))均低于对照组,针剌足三里其延髓MTL含量明显高于模型组(59±10vs47±13,P<0.05).三穴比较针剌四白穴主要影响胃窦MTL含量,针刺天枢主要影响胃窦SP含量变化,针剌足三里穴既使胃窦SP升高又使延髓MTL含量升高。结论针剌足阳明经对胃运动的调整作用有脑肠肽参与,但上述三穴对胃运动的影响所涉及的脑肠肽不完全一致。
