Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellit...Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. ehinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.展开更多
We studied oviposition sites and their effects on the reproductive success of the Chinhai salamander (Echinotriton chinhaiensis) .The results showed that: (1) the female had strong preferences for spawning sites and o...We studied oviposition sites and their effects on the reproductive success of the Chinhai salamander (Echinotriton chinhaiensis) .The results showed that: (1) the female had strong preferences for spawning sites and oviposition microhabitat; (2) the most suitable oviposition sites were 30 cm higher than water level and 45 cm from the water body; (3) oviposition sites usually overlapped;(4) embryos could develop and hatch normally in water as well as on land. The hatching rate on land was high (94 3%), but the number of hatchlings that successfully migrated to water was low (52%±11%, n =3 in the laboratory, and approximatly 20% in nature). Based on the situation in laboratory, the average hatchling survival rate at all oviposition sites was 36 7%. Owing to oviposition on land, the reproductive success of Chinhai salamander was very low;(5) protecting the spawning sites and increasing the hatchling survival rate are the keys to conserving this species.展开更多
基金financially supported by the National Natural Science Foundation of China (30770316)
文摘Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. ehinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.
文摘We studied oviposition sites and their effects on the reproductive success of the Chinhai salamander (Echinotriton chinhaiensis) .The results showed that: (1) the female had strong preferences for spawning sites and oviposition microhabitat; (2) the most suitable oviposition sites were 30 cm higher than water level and 45 cm from the water body; (3) oviposition sites usually overlapped;(4) embryos could develop and hatch normally in water as well as on land. The hatching rate on land was high (94 3%), but the number of hatchlings that successfully migrated to water was low (52%±11%, n =3 in the laboratory, and approximatly 20% in nature). Based on the situation in laboratory, the average hatchling survival rate at all oviposition sites was 36 7%. Owing to oviposition on land, the reproductive success of Chinhai salamander was very low;(5) protecting the spawning sites and increasing the hatchling survival rate are the keys to conserving this species.
