Detection of estrogenic endocrine disrupting compounds （EDCs） and N-nitrosodimethylamine （NDMA） in drinking water has led to rising concerns. There are, however, a paucity of studies on the distribution and season...Detection of estrogenic endocrine disrupting compounds （EDCs） and N-nitrosodimethylamine （NDMA） in drinking water has led to rising concerns. There are, however, a paucity of studies on the distribution and seasonal variation of NDMA and NDMA formation potential （NDMA-FP） in natural waters, especially in China. For EDCs, limited studies have investigated the distribution and seasonal variation of estrone （El）, 1713-estradiol （E2）, estriol （E3）, 17a-ethinylestradiol （EE2）, technical-nonylphenols （t-NP）, and bisphenol A （BPA） in Shanghai. In this study, water samples were collected from 11 sampling sites along the Huangpu River in 2012. The distribution and seasonal variation of EDCs, NDMA, and NDMA-FP were investigated. The results showed that all of the 11 sampling sites were contaminated by the target compounds. Compared with E2 and EE2, higher E1 and E3 concentrations were usually detected in the Huangpu River. The values of 17β- estradiol equivalents （EEQ） suggest a high possibility of endocrine effects on exposed organisms in the Huangpu River. NDMA-FP and t-NP were the dominant contaminants among the eight target compounds. The detection rates of the target compounds and their concentrations were both higher in dry seasons than in wet seasons. Higher concentrations of target compounds were observed in urban sampling sites near drainage outlets, and also in suburban sampling sites where intensive livestock farming and farmlands were located.展开更多
Studies on estrogenic disrupting compounds （EDCs） occurrence and identification of main responsible compounds in river water discharged into the sea are of significance. In the present research, we screened estrogen...Studies on estrogenic disrupting compounds （EDCs） occurrence and identification of main responsible compounds in river water discharged into the sea are of significance. In the present research, we screened estrogenic activities of 10 river water samples from 3 main rivers discharged into Bohai Sea in Tianjin using a recombinant two-hybrid yeast assay and chemical analysis by gas chromatography-mass spectrometry. All sample extracts induced significant estrogenic activity, with 17^-estradiol equivalents （EEQ） of raw water ranging from 5.72 to 59.06 ng/L. Six most concerned EDCs in the fiver water samples including estrone, 17（5-estradiol, 17ct-ethinylestradiol, estriol, diethylstilbestrol and estradiol valerate were determined, with their concentrations up to 50.70, 31.40, 24.40, 37.20, 2.56, and 8.47 ng/L, respectively. Through causality analysis by comparing the EEQ values of yeast assay and chemical analysis, 17ct-ethinylestradiol and 17[3-estradiol were identified as the main contributors to the estrogenic effects of the river samples, accounting for the whole estrogenic activities （62.99% to 185.66%）, and estrogen antagonistic compounds might presented in the heavy polluted water samples. The proposed approach using both chemical analysis and bioassay could be used for identification and evaluation of the estrogenic activity of EDCs in river water.展开更多
基金supported by the National Hi-Tech Research and Development Program(863)of China(No.2011AA060902)the Science and Technology Commission of Shanghai Municipality(No.11230700700)the National Natural Science Foundation of China(No.50878165)
文摘Detection of estrogenic endocrine disrupting compounds （EDCs） and N-nitrosodimethylamine （NDMA） in drinking water has led to rising concerns. There are, however, a paucity of studies on the distribution and seasonal variation of NDMA and NDMA formation potential （NDMA-FP） in natural waters, especially in China. For EDCs, limited studies have investigated the distribution and seasonal variation of estrone （El）, 1713-estradiol （E2）, estriol （E3）, 17a-ethinylestradiol （EE2）, technical-nonylphenols （t-NP）, and bisphenol A （BPA） in Shanghai. In this study, water samples were collected from 11 sampling sites along the Huangpu River in 2012. The distribution and seasonal variation of EDCs, NDMA, and NDMA-FP were investigated. The results showed that all of the 11 sampling sites were contaminated by the target compounds. Compared with E2 and EE2, higher E1 and E3 concentrations were usually detected in the Huangpu River. The values of 17β- estradiol equivalents （EEQ） suggest a high possibility of endocrine effects on exposed organisms in the Huangpu River. NDMA-FP and t-NP were the dominant contaminants among the eight target compounds. The detection rates of the target compounds and their concentrations were both higher in dry seasons than in wet seasons. Higher concentrations of target compounds were observed in urban sampling sites near drainage outlets, and also in suburban sampling sites where intensive livestock farming and farmlands were located.
基金supported by the National High Technology Research and Development Program (863) of China (No.2009AA06Z402)the National Science and Technology Major Project of China (No. 2008ZX07209-010)the National Natural Science Foundation of China (No.20921063)
文摘Studies on estrogenic disrupting compounds （EDCs） occurrence and identification of main responsible compounds in river water discharged into the sea are of significance. In the present research, we screened estrogenic activities of 10 river water samples from 3 main rivers discharged into Bohai Sea in Tianjin using a recombinant two-hybrid yeast assay and chemical analysis by gas chromatography-mass spectrometry. All sample extracts induced significant estrogenic activity, with 17^-estradiol equivalents （EEQ） of raw water ranging from 5.72 to 59.06 ng/L. Six most concerned EDCs in the fiver water samples including estrone, 17（5-estradiol, 17ct-ethinylestradiol, estriol, diethylstilbestrol and estradiol valerate were determined, with their concentrations up to 50.70, 31.40, 24.40, 37.20, 2.56, and 8.47 ng/L, respectively. Through causality analysis by comparing the EEQ values of yeast assay and chemical analysis, 17ct-ethinylestradiol and 17[3-estradiol were identified as the main contributors to the estrogenic effects of the river samples, accounting for the whole estrogenic activities （62.99% to 185.66%）, and estrogen antagonistic compounds might presented in the heavy polluted water samples. The proposed approach using both chemical analysis and bioassay could be used for identification and evaluation of the estrogenic activity of EDCs in river water.
