目的:探讨脑通汤对脑微血管内皮细胞(BMECs)缺氧/复氧损伤的保护作用及血脑屏障通透性的影响。方法:体外培养大鼠原代BMECs,缺氧/复氧法模拟脑缺血再灌注损伤。免疫组化检测内皮屏障抗原(EBA)蛋白表达;实时荧光定量PCR法检测紧密连接蛋...目的:探讨脑通汤对脑微血管内皮细胞(BMECs)缺氧/复氧损伤的保护作用及血脑屏障通透性的影响。方法:体外培养大鼠原代BMECs,缺氧/复氧法模拟脑缺血再灌注损伤。免疫组化检测内皮屏障抗原(EBA)蛋白表达;实时荧光定量PCR法检测紧密连接蛋白-5(Claudin-5)m RNA、紧密连接蛋白(ZO-1)m RNA的表达。结果:免疫组化示,与正常细胞组比较,模型组EBA蛋白表达明显下降(P<0.05);与模型组比较,脑通汤大、中剂量含药血清组EBA蛋白表达显著升高(P<0.05);但正常血清组与模型组比较差异无显著性(P>0.05)。实时荧光定量PCR法示,与正常细胞组比较,模型组Claudin-5、ZO-1 m RNA的表达明显下降(P<0.05);与模型组比较,脑通汤不同剂量含药血清组干预后,Claudin-5、ZO-1 m RNA的表达量明显上调,其中脑通汤大剂量含药血清组显著上调(P<0.05)。结论:脑通汤可以保护脑微血管内皮细胞缺氧/复氧损伤,其保护机制可能与其上调EBA蛋白及Claudin-5、ZO-1基因的表达密切相关。展开更多
To achieve the Sustainable Development Goals(SDGs), thereby meet the post 2020 global biodiversity targets and increase resilience to climate change, nature-based approaches such as ecosystem-based adaptation(EbA) is ...To achieve the Sustainable Development Goals(SDGs), thereby meet the post 2020 global biodiversity targets and increase resilience to climate change, nature-based approaches such as ecosystem-based adaptation(EbA) is suggested as a promising and integrated adaptation strategy. EbA comprises adaptation strategies that value the role of ecosystems in reducing social vulnerability to climate change. Among the different biological groups on earth, fungi play not only an important role to maintain the biogeochemical cycle/nutrient cycle in ecosystems(supporting and regulating services), but also contribute to the socio-economic and cultural benefits of societies(provisioning and cultural services). Here, we present our knowledge and scientific understanding on how these neglected groups of biodiversity-fungi are crucial for ecosystem-based adaptation(EbA) approach based on our field experience, review and associated expertise on caterpillar fungus(Ophiocordyceps sinensis), and other wild mushrooms found in Nepal. Several species of fungi are used by local communities as food, medicines, and environmental income. Fungi are important sources of household income for mountain communities in Nepal providing a cushion during shocks and disasters and supporting food security, health care, education and building shelter. For the holistic EbA approach, it is essential to strengthen local institutions as well as indigenous local knowledge which could be an important policy intervention for the identification, conservation, and sustainable management of ecologically, socially and economically useful fungal species.展开更多
The power consumption by the data cache is important in DSP designs. This study presents an enhanced branch access LRU-SEQ （EBA-LRU-SEQ） policy for data caches in DSP designs to reduce the power consumption. The des...The power consumption by the data cache is important in DSP designs. This study presents an enhanced branch access LRU-SEQ （EBA-LRU-SEQ） policy for data caches in DSP designs to reduce the power consumption. The design is based on the LRU policy with embedded prefetch table to provide branch access. Tests show that the EBA-LRU-SEQ policy reduces the data cache power consumption to 54% of a system with no power control.展开更多
Background: Epidermolysis bullosa aquisita (EBA) is a severe acquired blistering skin disease that is often resistant to prednisolone but can respond well to intravenous immunoglobulin infusion (IVIg). Main Observatio...Background: Epidermolysis bullosa aquisita (EBA) is a severe acquired blistering skin disease that is often resistant to prednisolone but can respond well to intravenous immunoglobulin infusion (IVIg). Main Observations: We describe the case of a 35 years old male patient with EBA who developed clinically significant haemolytic anaemia with a drop in Hb from 15.3 g/dL to a nadir of 8.4 g/dL within 5 days post IVIg infusion. The patient was blood group A and the IVIg batch was found to have a high titre of anti-A immunoglobulin. Conclusions: IVIg is an effective treatment for EBA. Haemolysis associated with IVIg has not previously been reported in the dermatology literature but review of data from other specialties shows that the problem is well recognised. Dermatologists using IVIg should be aware of this potential complication and patients should be consented appropriately and warned about this potential side effect.展开更多
文摘目的:探讨脑通汤对脑微血管内皮细胞(BMECs)缺氧/复氧损伤的保护作用及血脑屏障通透性的影响。方法:体外培养大鼠原代BMECs,缺氧/复氧法模拟脑缺血再灌注损伤。免疫组化检测内皮屏障抗原(EBA)蛋白表达;实时荧光定量PCR法检测紧密连接蛋白-5(Claudin-5)m RNA、紧密连接蛋白(ZO-1)m RNA的表达。结果:免疫组化示,与正常细胞组比较,模型组EBA蛋白表达明显下降(P<0.05);与模型组比较,脑通汤大、中剂量含药血清组EBA蛋白表达显著升高(P<0.05);但正常血清组与模型组比较差异无显著性(P>0.05)。实时荧光定量PCR法示,与正常细胞组比较,模型组Claudin-5、ZO-1 m RNA的表达明显下降(P<0.05);与模型组比较,脑通汤不同剂量含药血清组干预后,Claudin-5、ZO-1 m RNA的表达量明显上调,其中脑通汤大剂量含药血清组显著上调(P<0.05)。结论:脑通汤可以保护脑微血管内皮细胞缺氧/复氧损伤,其保护机制可能与其上调EBA蛋白及Claudin-5、ZO-1基因的表达密切相关。
基金The Global Biodiversity Information Facility/Biodiversity Fund for Asia (BIFA5_023 to SD)The Rufford Foundation (25337-1 to SD)The National Geographic Society (NGS-62058R-19 to UBS)。
文摘To achieve the Sustainable Development Goals(SDGs), thereby meet the post 2020 global biodiversity targets and increase resilience to climate change, nature-based approaches such as ecosystem-based adaptation(EbA) is suggested as a promising and integrated adaptation strategy. EbA comprises adaptation strategies that value the role of ecosystems in reducing social vulnerability to climate change. Among the different biological groups on earth, fungi play not only an important role to maintain the biogeochemical cycle/nutrient cycle in ecosystems(supporting and regulating services), but also contribute to the socio-economic and cultural benefits of societies(provisioning and cultural services). Here, we present our knowledge and scientific understanding on how these neglected groups of biodiversity-fungi are crucial for ecosystem-based adaptation(EbA) approach based on our field experience, review and associated expertise on caterpillar fungus(Ophiocordyceps sinensis), and other wild mushrooms found in Nepal. Several species of fungi are used by local communities as food, medicines, and environmental income. Fungi are important sources of household income for mountain communities in Nepal providing a cushion during shocks and disasters and supporting food security, health care, education and building shelter. For the holistic EbA approach, it is essential to strengthen local institutions as well as indigenous local knowledge which could be an important policy intervention for the identification, conservation, and sustainable management of ecologically, socially and economically useful fungal species.
文摘The power consumption by the data cache is important in DSP designs. This study presents an enhanced branch access LRU-SEQ （EBA-LRU-SEQ） policy for data caches in DSP designs to reduce the power consumption. The design is based on the LRU policy with embedded prefetch table to provide branch access. Tests show that the EBA-LRU-SEQ policy reduces the data cache power consumption to 54% of a system with no power control.
文摘Background: Epidermolysis bullosa aquisita (EBA) is a severe acquired blistering skin disease that is often resistant to prednisolone but can respond well to intravenous immunoglobulin infusion (IVIg). Main Observations: We describe the case of a 35 years old male patient with EBA who developed clinically significant haemolytic anaemia with a drop in Hb from 15.3 g/dL to a nadir of 8.4 g/dL within 5 days post IVIg infusion. The patient was blood group A and the IVIg batch was found to have a high titre of anti-A immunoglobulin. Conclusions: IVIg is an effective treatment for EBA. Haemolysis associated with IVIg has not previously been reported in the dermatology literature but review of data from other specialties shows that the problem is well recognised. Dermatologists using IVIg should be aware of this potential complication and patients should be consented appropriately and warned about this potential side effect.
