探讨固肾安胎丸联用黄体酮治疗先兆流产的临床疗效,为临床早期干预先兆流产提供思路和借鉴。选择收集的先兆流产患者共112例,随机分成对照组（n=56）和观察组（n=56）,对照组患者常规给予黄体酮针,观察组在对照组基础上再口服固肾安胎...探讨固肾安胎丸联用黄体酮治疗先兆流产的临床疗效,为临床早期干预先兆流产提供思路和借鉴。选择收集的先兆流产患者共112例,随机分成对照组（n=56）和观察组（n=56）,对照组患者常规给予黄体酮针,观察组在对照组基础上再口服固肾安胎丸,2组均采用药物治疗2周,并于治疗前及治疗后1-2周抽取静脉血5 m L,测定血清β-HCG,P,E2和CA125水平,比较治疗后2组间的差异。观察组的有效率为91.9%,对照组为79%,2组的有效率比较,差异有统计学意义（P〈0.05）。治疗2周后,观察组血清P和E2水平均较治疗前明显上升,血清CA125水平显著下降（P〈0.05）,与同期对照组的这些指标比较,差异有统计学意义（P〈0.05）;治疗后2组血清β-HCG水平较治疗前均显著上升（P〈0.05）,但2组该水平比较,差异无统计学意义。固肾安胎丸联用黄体酮治疗先兆流产具有较好的临床疗效,可显著提升血清β-HCG,P,E2水平,降低血清CA125水平,增加保胎的有效率,值得临床推广应用。展开更多
AIM： To investigate the effect of short-chain fatty acids （SCFAs） on production of prostaglandin E2 （PGE2）, cytokines and chemokines in human monocytes. METHODS： Human neutrophils and monocytes were isolated fro...AIM： To investigate the effect of short-chain fatty acids （SCFAs） on production of prostaglandin E2 （PGE2）, cytokines and chemokines in human monocytes. METHODS： Human neutrophils and monocytes were isolated from human whole blood by using 1-Step Polymorph and RosetteSep Human Monocyte Enrichment Cocktail, respectively. Human GPR41 and GPR43 mRNA expression was examined by quantitative realtime polymerase chain reaction, The calcium flux assay was used to examine the biological activities of SCFAs in human neutrophils and monocytes. The effect of SCFAs on human monocytes and peripheral blood mononuclear cells （PBMC） was studied by measuring PGE2, cytokines and chemokines in the supernatant. The effect of SCFAs in vivo was examined by intraplantar injection into rat paws. RESULTS： Human GPR43 is highly expressed in human neutrophils and monocytes. SCFAs induce robust calcium flux in human neutrophils, but not in human monocytes. In this study, we show that SCFAs can induce human monocyte release of PGE2 and that this effect can be enhanced in the presence of lipopolysaccharide （LPS）. In addition, we demonstrate that PGE2 production induced by SCFA was inhibited by pertussis toxin, suggesting the involvement of a receptor-mediated mechanism. Furthermore, SCFAs can specifically inhibit constitutive monocyte chemotactic protein-1 （MCP-1） production and LPS-induced interleukin-10 （IL-10） production in human monocytes without affecting the secretion of other cytokines and chemokines examined. Similar activities were observed in human PBMC for the release of PGE2, MCP-1 and IL-10 after 5CFA treatment. In addition, SCFAs inhibit LPS-induced production of tumor necrosis factor-α and interferon-7 in human PBIVlC. Finally, we show that SCFAs and LPS can induce PGE2 production in vivo by intraplantar injection into rat paws （P 〈 0.01）. CONCLUSION： SCFAs can have distinct antiinflammatory activities due to their regulation of PGE2, cytokine and chemokine release from human immune cells.展开更多
目的:探讨不同孕期孕妇血清中不同激素水平与妊娠结局的相关性,旨在为血清学联检筛查胎儿发育异常的诊断方法提供依据。方法:采用横断面研究方法,选取唐山工人医院门诊常规孕前检查的孕妇400例,收集不同孕周(早期妊娠100例,中期妊娠200...目的:探讨不同孕期孕妇血清中不同激素水平与妊娠结局的相关性,旨在为血清学联检筛查胎儿发育异常的诊断方法提供依据。方法:采用横断面研究方法,选取唐山工人医院门诊常规孕前检查的孕妇400例,收集不同孕周(早期妊娠100例,中期妊娠200例,晚期妊娠100例)孕妇的外周血,应用全自动化学发光免疫分析仪检测不同孕期孕妇血清中人绒毛膜促性腺激素(hCG)、雌二醇(E2)、孕酮(PROG)、甲胎蛋白(AFP)、游离雌三醇(uE3)、催乳素(PRL)、黄体生成素(LH)、卵泡刺激素(FSH)水平,并跟踪妊娠结局,比较不同孕期不同妊娠结局组孕妇血清各激素水平差异性。结果:调查结果显示,400例不同孕期的孕妇中,正常妊娠结局者364例,占91%;早期妊娠的主要不良妊娠结局为流产和胚胎停育,且流产、胚胎停育孕妇血清hCG、PROG、E2水平低于早期正常妊娠孕妇,差异有统计学意义(22 570±6 219、5 997±1 790 vs 89430±19 689,18.36±4.58、10.87±1.42 vs 37.68±12.02,756±153、282±129 vs 1 270±220,P<0.001);胚胎停育孕妇血清h CG、PROG、E2水平低于流产孕妇,差异有统计学意义(5 997±1 790 vs 22 570±6 219,10.87±1.42vs18.36,282±129 vs 756±153,均P<0.05);中期妊娠的主要不良妊娠结局为唐氏综合征,且唐氏综合征孕妇血清h CG水平高于中期正常妊娠孕妇,差异有统计学意义(71 500±13 787 vs 43 720±16 038,P<0.01),血清u E3、AFP水平低于中期正常妊娠孕妇,差异有统计学意义(0.75±0.14 vs 2.94±1.41,21.69±3.29 vs 54.08±23.34,均P<0.05);晚期妊娠的主要不良妊娠结局为妊娠期高血压疾病,且妊娠期高血压疾病孕妇血清h CG、AFP水平高于晚期正常妊娠孕妇,差异有统计学意义(30 700±2 662 vs 25 389±8855,179.33±27.82 vs 104.15±23.10,均P<0.001);不同妊娠期各激素相关性分析中,流产孕妇中血清h CG与PROG呈正相关,r=0.660。结论:妊娠早期血清h CG、PROG、E2水平降低与流产及胚胎停育有关展开更多
文摘探讨固肾安胎丸联用黄体酮治疗先兆流产的临床疗效,为临床早期干预先兆流产提供思路和借鉴。选择收集的先兆流产患者共112例,随机分成对照组（n=56）和观察组（n=56）,对照组患者常规给予黄体酮针,观察组在对照组基础上再口服固肾安胎丸,2组均采用药物治疗2周,并于治疗前及治疗后1-2周抽取静脉血5 m L,测定血清β-HCG,P,E2和CA125水平,比较治疗后2组间的差异。观察组的有效率为91.9%,对照组为79%,2组的有效率比较,差异有统计学意义（P〈0.05）。治疗2周后,观察组血清P和E2水平均较治疗前明显上升,血清CA125水平显著下降（P〈0.05）,与同期对照组的这些指标比较,差异有统计学意义（P〈0.05）;治疗后2组血清β-HCG水平较治疗前均显著上升（P〈0.05）,但2组该水平比较,差异无统计学意义。固肾安胎丸联用黄体酮治疗先兆流产具有较好的临床疗效,可显著提升血清β-HCG,P,E2水平,降低血清CA125水平,增加保胎的有效率,值得临床推广应用。
文摘AIM： To investigate the effect of short-chain fatty acids （SCFAs） on production of prostaglandin E2 （PGE2）, cytokines and chemokines in human monocytes. METHODS： Human neutrophils and monocytes were isolated from human whole blood by using 1-Step Polymorph and RosetteSep Human Monocyte Enrichment Cocktail, respectively. Human GPR41 and GPR43 mRNA expression was examined by quantitative realtime polymerase chain reaction, The calcium flux assay was used to examine the biological activities of SCFAs in human neutrophils and monocytes. The effect of SCFAs on human monocytes and peripheral blood mononuclear cells （PBMC） was studied by measuring PGE2, cytokines and chemokines in the supernatant. The effect of SCFAs in vivo was examined by intraplantar injection into rat paws. RESULTS： Human GPR43 is highly expressed in human neutrophils and monocytes. SCFAs induce robust calcium flux in human neutrophils, but not in human monocytes. In this study, we show that SCFAs can induce human monocyte release of PGE2 and that this effect can be enhanced in the presence of lipopolysaccharide （LPS）. In addition, we demonstrate that PGE2 production induced by SCFA was inhibited by pertussis toxin, suggesting the involvement of a receptor-mediated mechanism. Furthermore, SCFAs can specifically inhibit constitutive monocyte chemotactic protein-1 （MCP-1） production and LPS-induced interleukin-10 （IL-10） production in human monocytes without affecting the secretion of other cytokines and chemokines examined. Similar activities were observed in human PBMC for the release of PGE2, MCP-1 and IL-10 after 5CFA treatment. In addition, SCFAs inhibit LPS-induced production of tumor necrosis factor-α and interferon-7 in human PBIVlC. Finally, we show that SCFAs and LPS can induce PGE2 production in vivo by intraplantar injection into rat paws （P 〈 0.01）. CONCLUSION： SCFAs can have distinct antiinflammatory activities due to their regulation of PGE2, cytokine and chemokine release from human immune cells.
文摘目的:探讨不同孕期孕妇血清中不同激素水平与妊娠结局的相关性,旨在为血清学联检筛查胎儿发育异常的诊断方法提供依据。方法:采用横断面研究方法,选取唐山工人医院门诊常规孕前检查的孕妇400例,收集不同孕周(早期妊娠100例,中期妊娠200例,晚期妊娠100例)孕妇的外周血,应用全自动化学发光免疫分析仪检测不同孕期孕妇血清中人绒毛膜促性腺激素(hCG)、雌二醇(E2)、孕酮(PROG)、甲胎蛋白(AFP)、游离雌三醇(uE3)、催乳素(PRL)、黄体生成素(LH)、卵泡刺激素(FSH)水平,并跟踪妊娠结局,比较不同孕期不同妊娠结局组孕妇血清各激素水平差异性。结果:调查结果显示,400例不同孕期的孕妇中,正常妊娠结局者364例,占91%;早期妊娠的主要不良妊娠结局为流产和胚胎停育,且流产、胚胎停育孕妇血清hCG、PROG、E2水平低于早期正常妊娠孕妇,差异有统计学意义(22 570±6 219、5 997±1 790 vs 89430±19 689,18.36±4.58、10.87±1.42 vs 37.68±12.02,756±153、282±129 vs 1 270±220,P<0.001);胚胎停育孕妇血清h CG、PROG、E2水平低于流产孕妇,差异有统计学意义(5 997±1 790 vs 22 570±6 219,10.87±1.42vs18.36,282±129 vs 756±153,均P<0.05);中期妊娠的主要不良妊娠结局为唐氏综合征,且唐氏综合征孕妇血清h CG水平高于中期正常妊娠孕妇,差异有统计学意义(71 500±13 787 vs 43 720±16 038,P<0.01),血清u E3、AFP水平低于中期正常妊娠孕妇,差异有统计学意义(0.75±0.14 vs 2.94±1.41,21.69±3.29 vs 54.08±23.34,均P<0.05);晚期妊娠的主要不良妊娠结局为妊娠期高血压疾病,且妊娠期高血压疾病孕妇血清h CG、AFP水平高于晚期正常妊娠孕妇,差异有统计学意义(30 700±2 662 vs 25 389±8855,179.33±27.82 vs 104.15±23.10,均P<0.001);不同妊娠期各激素相关性分析中,流产孕妇中血清h CG与PROG呈正相关,r=0.660。结论:妊娠早期血清h CG、PROG、E2水平降低与流产及胚胎停育有关
