OBJECTIVE： The increasing popularity and widespread use of traditional Chinese herbs as alterna- tive medicine have sparked an interest in understanding their biosafety, especially in decoctions that are consumed. Th...OBJECTIVE： The increasing popularity and widespread use of traditional Chinese herbs as alterna- tive medicine have sparked an interest in understanding their biosafety, especially in decoctions that are consumed. This study aimed to assess the level of microbial and heavy metal contamination in commonly consumed herbal medicine in Malay- sia and the effects of boiling on these contamination levels. METHODS： Four commonly consumed Chinese herbal medicine in Malaysia-＂Eight Treasure Herbal Tea＂, ＂Herbal Tea＂, Xiyangshen （Radix Panacis Quin- quefolii） and Dangshen （Radix Codonopsis） were evaluated in this study. Herbal medicines were pre- pared as boiled and non-boiled decoctions, and their microbial enumeration and heavy metal detection were conducted with plate assay and atom- ic absorption spectroscopy, respectively. RESULTS： Findings revealed that herbal medicines generally had 6 Iogl0cfu/mL microbial cells and that boiling had significantly reduced microbial contaminants, where no Bacillus spp., Staphylococcus spp. and Clostridium spp. were recovered. Heavy metals such as Mn, Cu, Cd, Pb, Fe and Zn were also detect- ed from all the samples, generally in low concentra- tions （〈1 mg/L） except for Mn （18.545 mg/L）. All de- coctions （after boiling） have reduced concentra- tions of Cu, while others were not significantly dif- ferent. Comparisons between samples with single and multi-herbs suggest level of microbial and metal contamination is not influenced by number of herbs in sample. CONCLUSION： Herbal medicines generally have microbial and heavy metal contaminants. However, the boiling process to generate decoctions was able to successfully reduce the number of microbes and Cu, ensuring safety of herbal medicines for consumption.展开更多
目的评估在静配中心应用密闭式药物配制和转运系统(closed-system drug transfer device,CSTD)对细胞毒性药物在工作环境中污染残留量的影响。方法利用环磷酰胺为示例药物,通过WIPE采样法和高效液相色谱法定量检测复旦大学附属肿瘤医院...目的评估在静配中心应用密闭式药物配制和转运系统(closed-system drug transfer device,CSTD)对细胞毒性药物在工作环境中污染残留量的影响。方法利用环磷酰胺为示例药物,通过WIPE采样法和高效液相色谱法定量检测复旦大学附属肿瘤医院静配中心配制舱内环境中环磷酰胺残留量。在常规配制方法配药的一个工作日配药结束时清理前、清理后以及连续应用3周CSTD进行配药工作后,分别进行3批采样。采样位置包括3个生物安全柜(BSC)操作台面、转运小推车、出舱台等19个位置。比较3批样本中环磷酰胺的残留量。结果常规配药清理去污前各监测点中位值为1.30(IRQ:0.24,2.60)ng/cm^2,去污后中位值为0.22(IRQ:0.08,0.53)ng/cm^2,前后比较差异有统计学意义(P<0.001)。CSTD系统配药后,各监测点中位值为0.06(0.02,0.88)ng/cm^2,明显低于常规方法去污前(P<0.001),也低于常规方法去污后,但差异无统计学意义(P=0.299)。结论应用CSTD可有效减少细胞毒性药物在配制转运过程中的渗漏污染,从而显著减少相关医务人员的毒性药物职业暴露。展开更多
目的建立硫酸沙丁胺醇原料药有关物质的检测方法。方法采用高效液相色谱(HPLC)法测定。色谱柱为Hypersil Gold C8柱(250 mm×4.6 mm,3μm),流动相A为3.45 g一水合磷酸二氢钠用900 m L 0.05%的三乙酸胺溶液溶解,以稀磷酸调节pH至3.0...目的建立硫酸沙丁胺醇原料药有关物质的检测方法。方法采用高效液相色谱(HPLC)法测定。色谱柱为Hypersil Gold C8柱(250 mm×4.6 mm,3μm),流动相A为3.45 g一水合磷酸二氢钠用900 m L 0.05%的三乙酸胺溶液溶解,以稀磷酸调节pH至3.0之后用0.05%的三乙酸胺溶液定容至1 000 mL;流动相B为甲醇∶乙腈(体积比20∶80),进行梯度洗脱;流速为1.0mL/min,检测波长为273 nm,柱温为25℃。结果在该色谱条件下,硫酸沙丁胺醇及杂质A、B、C、D、E、F、G、K、M、O、L、Q、I、J均能有效分离,分离度均大于1.5;并且硫酸沙丁胺醇及杂质C、D、F、M、O在0.45~18.0μg/mL内质量浓度与峰面积线性关系良好,回收率分别为杂质C为99.1%,RSD为2.6%(n=9);杂质D为99.3%,RSD为2.1%(n=9);杂质F为100.1%,RSD为2.5%(n=9);杂质M为100.5%,RSD为1.7%(n=9);杂质O为100.2%,RSD为1.9%(n=9)。结论该方法专属性强,适用于硫酸沙丁胺醇原料药有关物质检测。展开更多
文摘OBJECTIVE： The increasing popularity and widespread use of traditional Chinese herbs as alterna- tive medicine have sparked an interest in understanding their biosafety, especially in decoctions that are consumed. This study aimed to assess the level of microbial and heavy metal contamination in commonly consumed herbal medicine in Malay- sia and the effects of boiling on these contamination levels. METHODS： Four commonly consumed Chinese herbal medicine in Malaysia-＂Eight Treasure Herbal Tea＂, ＂Herbal Tea＂, Xiyangshen （Radix Panacis Quin- quefolii） and Dangshen （Radix Codonopsis） were evaluated in this study. Herbal medicines were pre- pared as boiled and non-boiled decoctions, and their microbial enumeration and heavy metal detection were conducted with plate assay and atom- ic absorption spectroscopy, respectively. RESULTS： Findings revealed that herbal medicines generally had 6 Iogl0cfu/mL microbial cells and that boiling had significantly reduced microbial contaminants, where no Bacillus spp., Staphylococcus spp. and Clostridium spp. were recovered. Heavy metals such as Mn, Cu, Cd, Pb, Fe and Zn were also detect- ed from all the samples, generally in low concentra- tions （〈1 mg/L） except for Mn （18.545 mg/L）. All de- coctions （after boiling） have reduced concentra- tions of Cu, while others were not significantly dif- ferent. Comparisons between samples with single and multi-herbs suggest level of microbial and metal contamination is not influenced by number of herbs in sample. CONCLUSION： Herbal medicines generally have microbial and heavy metal contaminants. However, the boiling process to generate decoctions was able to successfully reduce the number of microbes and Cu, ensuring safety of herbal medicines for consumption.
文摘目的评估在静配中心应用密闭式药物配制和转运系统(closed-system drug transfer device,CSTD)对细胞毒性药物在工作环境中污染残留量的影响。方法利用环磷酰胺为示例药物,通过WIPE采样法和高效液相色谱法定量检测复旦大学附属肿瘤医院静配中心配制舱内环境中环磷酰胺残留量。在常规配制方法配药的一个工作日配药结束时清理前、清理后以及连续应用3周CSTD进行配药工作后,分别进行3批采样。采样位置包括3个生物安全柜(BSC)操作台面、转运小推车、出舱台等19个位置。比较3批样本中环磷酰胺的残留量。结果常规配药清理去污前各监测点中位值为1.30(IRQ:0.24,2.60)ng/cm^2,去污后中位值为0.22(IRQ:0.08,0.53)ng/cm^2,前后比较差异有统计学意义(P<0.001)。CSTD系统配药后,各监测点中位值为0.06(0.02,0.88)ng/cm^2,明显低于常规方法去污前(P<0.001),也低于常规方法去污后,但差异无统计学意义(P=0.299)。结论应用CSTD可有效减少细胞毒性药物在配制转运过程中的渗漏污染,从而显著减少相关医务人员的毒性药物职业暴露。
