A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOP...A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOPE）. This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K2HPO4 concentration,E. coli cel l concentration,pH and treatment time on the release of L-asparaginase were inv estigated.Under suitable conditions,over 90% of L-asparaginase in cells was re leased into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner str ucture of E.coli cells significantly.展开更多
研究了三乙醇胺(TEA)水溶液在电解质作用下的相分离及其通过形成TEA-Fe3+配合物实现三乙醇胺相对Fe3+的萃取,萃取过程将萃取剂和萃取溶剂合二为一。同时还讨论了表面张力、溶液提升率对火焰原子吸收光谱法(FAAS)测定三乙醇胺相中铁的影...研究了三乙醇胺(TEA)水溶液在电解质作用下的相分离及其通过形成TEA-Fe3+配合物实现三乙醇胺相对Fe3+的萃取,萃取过程将萃取剂和萃取溶剂合二为一。同时还讨论了表面张力、溶液提升率对火焰原子吸收光谱法(FAAS)测定三乙醇胺相中铁的影响。建立了TEA-K2HPO4-H2O双水相萃取、FAAS测定铁的新方法:在试液中加入1 mL三乙醇胺(1+1)和5 g K2HPO4后以水定容为10 mL,离心分离后用FAAS测定三乙醇胺相中的铁。线性范围20～240μg/L,检出限6.1μg/L(S/N=3),已用于自来水和井水中铁的萃取。展开更多
文摘A method to release L-asparaginase（EC3.5.1.1） from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether （POOPE）. This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K2HPO4 concentration,E. coli cel l concentration,pH and treatment time on the release of L-asparaginase were inv estigated.Under suitable conditions,over 90% of L-asparaginase in cells was re leased into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner str ucture of E.coli cells significantly.
文摘研究了三乙醇胺(TEA)水溶液在电解质作用下的相分离及其通过形成TEA-Fe3+配合物实现三乙醇胺相对Fe3+的萃取,萃取过程将萃取剂和萃取溶剂合二为一。同时还讨论了表面张力、溶液提升率对火焰原子吸收光谱法(FAAS)测定三乙醇胺相中铁的影响。建立了TEA-K2HPO4-H2O双水相萃取、FAAS测定铁的新方法:在试液中加入1 mL三乙醇胺(1+1)和5 g K2HPO4后以水定容为10 mL,离心分离后用FAAS测定三乙醇胺相中的铁。线性范围20～240μg/L,检出限6.1μg/L(S/N=3),已用于自来水和井水中铁的萃取。
