The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics.In this field,microfluidic deterministic lateral displacement(DLD)holds a promise...The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics.In this field,microfluidic deterministic lateral displacement(DLD)holds a promise due to the ability of continuous separation of particles by size,shape,deformability,and electrical properties with high resolution.DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes.DLD techniques have been previously reviewed in 2014.Since then,the field has matured as several physics of DLD have been updated,new phenomena have been discovered,and various designs have been presented to achieve a higher separation performance and throughput.Furthermore,some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection.This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection.Furthermore,current challenges and potential solutions of DLD are also discussed.We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications.In particular,the rapid,low-cost,and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.展开更多
The deterministic lateral displacement (DLD) is an important method used to sort particles and cells of different sizes. In this paper, the flexible cell sorting with the DLD method is studied by using a numerical mod...The deterministic lateral displacement (DLD) is an important method used to sort particles and cells of different sizes. In this paper, the flexible cell sorting with the DLD method is studied by using a numerical model based on the immersed boundary-lattice Boltzmann method (IB-LBM). In this model, the fluid motion is solved by the LBM, and the cell membrane-fluid interaction is modeled with the LBM. The proposed model is validated by simulating the rigid particle sorted with the DLD method, and the results are found in good agreement with those measured in experiments. We first study the effect of flexibility on a single cell and multiple cells continuously going through a DLD device. It is found that the cell flexibility can significantly affect the cell path, which means the flexibility could have significant effects on the continuous cell sorting by the DLD method. The sorting characteristics of white blood cells and red blood cells are further studied by varying the spatial distribution of cylinder arrays and the initial cell-cell distance. The numerical results indicate that a well concentrated cell sorting can be obtained under a proper arrangement of cylinder arrays and a large enough initial cell-cell distance.展开更多
基金the scholarship from NUS Graduate School for integrative science and engineering and funding support from Ministry of Education Academic Research Fund,Singapore(AcRF:R-397-000-270-114,R-397-000-183-112).
文摘The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics.In this field,microfluidic deterministic lateral displacement(DLD)holds a promise due to the ability of continuous separation of particles by size,shape,deformability,and electrical properties with high resolution.DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes.DLD techniques have been previously reviewed in 2014.Since then,the field has matured as several physics of DLD have been updated,new phenomena have been discovered,and various designs have been presented to achieve a higher separation performance and throughput.Furthermore,some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection.This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection.Furthermore,current challenges and potential solutions of DLD are also discussed.We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications.In particular,the rapid,low-cost,and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.
基金supported by the National Natural Science Foundation of China (Grant 81301291)the Beijing Higher Education Young Elite Teacher Project (Grant YETP1208)UNSW Special Research Grants Program
文摘The deterministic lateral displacement (DLD) is an important method used to sort particles and cells of different sizes. In this paper, the flexible cell sorting with the DLD method is studied by using a numerical model based on the immersed boundary-lattice Boltzmann method (IB-LBM). In this model, the fluid motion is solved by the LBM, and the cell membrane-fluid interaction is modeled with the LBM. The proposed model is validated by simulating the rigid particle sorted with the DLD method, and the results are found in good agreement with those measured in experiments. We first study the effect of flexibility on a single cell and multiple cells continuously going through a DLD device. It is found that the cell flexibility can significantly affect the cell path, which means the flexibility could have significant effects on the continuous cell sorting by the DLD method. The sorting characteristics of white blood cells and red blood cells are further studied by varying the spatial distribution of cylinder arrays and the initial cell-cell distance. The numerical results indicate that a well concentrated cell sorting can be obtained under a proper arrangement of cylinder arrays and a large enough initial cell-cell distance.
