目的 :观察快动眼睡眠 ( rapid eye movement sleep,REMS)剥夺大鼠行为的改变及其下丘脑诱生型一氧化氮合酶( i NOS) m RNA表达的时相变化。方法 :小站台水环境法剥夺大鼠睡眠 2 4、4 8、72 h后 ,用旷场实验 ( open field test,OFT)观...目的 :观察快动眼睡眠 ( rapid eye movement sleep,REMS)剥夺大鼠行为的改变及其下丘脑诱生型一氧化氮合酶( i NOS) m RNA表达的时相变化。方法 :小站台水环境法剥夺大鼠睡眠 2 4、4 8、72 h后 ,用旷场实验 ( open field test,OFT)观察大鼠主动行为的变化 ,同时用半定量逆转录聚合酶链反应 ( RT- PCR)检测其下丘脑 i NOS m RNA的表达。结果 :睡眠剥夺2 4、4 8h组大鼠的旷场实验得分较非剥夺组高 ( P<0 .0 1) ,剥夺 72 h后降低 ;睡眠剥夺 2 4 h及 4 8h组 i NOS m RNA的表达量无显著变化 ,但剥夺 72 h组 i NOS m RNA的表达量大幅度增加 ( P<0 .0 1)。结论 :内源性一氧化氮 ( NO)增加可能是睡眠剥夺后机体精神行为变化的重要原因 ,高水平的展开更多
AIM: To investigate if sleep deprivation is able to increase the expression of inducible heat shock protein 70 in gastric mucosa and its possible role in mucosal defense. METHODS: Rats for sleep disruption were placed...AIM: To investigate if sleep deprivation is able to increase the expression of inducible heat shock protein 70 in gastric mucosa and its possible role in mucosal defense. METHODS: Rats for sleep disruption were placed inside a computerized rotating drum, gastric mucosa was taken from rats with 1, 3 and 7d sleep deprivation. RT-PCR, immunohistochemistry and Western blotting were used to determine the expression of heat shock protein 70. Ethanol (500mL.L(-1), i.g.) was used to induce gastric mucosa damage. RESULTS: RT-PCR, Western blotting and immunostaining confirmed that the sleep deprivation as a stress resulted in significantly greater expression of inducible heat shock protein 70 in gastric mucosa of rats. After the 500mL.L(-1) ethanol challenge, the ulcer area found in the rats with 7d sleep deprivation (19.15 +/- 4.2)mm(2) was significantly lower (P【0.01) than the corresponding control (53.7 +/- 8.1) mm(2). CONCLUSION: Sleep deprivation as a stress, in addition to lowering the gastric mucosal barrier, is able to stimulate the expression of inducible heat shock protein 70 in gastric mucosa of rats, the heat shock protein 70 may play an important role in gastric mucosal protection.展开更多
目的分析ICU患者睡眠障碍的研究文献,了解ICU患者睡眠障碍的发展趋势、研究热点,为ICU患者睡眠障碍相关研究提供借鉴和参考。方法在中国知网数据库(CNKI)和Web of science(WOS)数据库,以MeSH主题词和布尔逻辑运算符对ICU患者睡眠剥夺主...目的分析ICU患者睡眠障碍的研究文献,了解ICU患者睡眠障碍的发展趋势、研究热点,为ICU患者睡眠障碍相关研究提供借鉴和参考。方法在中国知网数据库(CNKI)和Web of science(WOS)数据库,以MeSH主题词和布尔逻辑运算符对ICU患者睡眠剥夺主题进行检索(时间为建库-2020年),应用可视化分析软件Citespace进行文献计量学分析。结果CNKI检索出398篇,WOS共检索出584篇,发文量总体上呈现增加的趋势,高频关键词为综合护理、集束护理、舒适护理、护理对策等,分析出的突显词为镇静镇痛、彼斯堡睡眠指数及综合护理等。结论ICU患者睡眠剥夺的护理日益受到护理研究人员的关注,ICU患者睡眠剥夺的综合护理的价值开始得到更多的认可。但ICU患者睡眠剥夺的研究测量工具在临床实验和实践中有待进一步的发展,对ICU患者睡眠剥夺的整体护理有待更多的临床实践去发展。展开更多
Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to b...Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern experiments.In this study,we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance model.We established a model of sleep rhythm disturbance in C57 BL/6 J mice via a sleep deprivation method involving manual cage tapping,cage jostling,and nest disturbance.We used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive function.Our results indicated that an intervention in which the mice were exposed to blue light(40-Hz flickering frequency)for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep deprivation.However,interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points,as well as 2 Hz-blue light at 3 intervention time points(subjective day time,subjective night time,and offset time points),had no positive effects on circadian rhythm shift or memory deficits.Additionally,a 2000-Hz sound intervention during subjective day time attenuated the24-hour-acrophase shift and memory decline,while 440-Hz and 4000-Hz sounds had no effect on circadian rhythms.Overall,these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake rhythm.All animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University,China(approval No.20171102)on November20,2017.展开更多
Sleep hygiene and knowledge about sleep basics is unknown in college student populations. The college student population have abbreviated sleep due to social schedules and busy academic schedules. A measurement of sle...Sleep hygiene and knowledge about sleep basics is unknown in college student populations. The college student population have abbreviated sleep due to social schedules and busy academic schedules. A measurement of sleep hygiene and knowledge about sleep in the college student population indicates that there are varying levels along with sleep knowledge. The objective of the study was to examine young adult sleep quality because of providing an educative intervention. A sleep class of one hour provided once a week for ten weeks was provided following the administration of a ten-item sleep survey. Following the sleep class, the sleep survey was read ministered. Measures of central tendency and non-parametric analysis of nominal data were conducted. The results indicated two of the five cohorts had statistically significant differences. Further, the measures of sleep quality differed, statistically, for two of the cohorts receiving the classroom instruction. This research finding indicates a continued need to educate college student populations about their sleep health.展开更多
INTRODUCTIONStress has been shown to induce gastric mucosallesions and lower the effectiveness of the mucosa asa barrier.In rats,gastric ulcers can beproduced by cold-restraint stress and it isfrequently employed as a...INTRODUCTIONStress has been shown to induce gastric mucosallesions and lower the effectiveness of the mucosa asa barrier.In rats,gastric ulcers can beproduced by cold-restraint stress and it isfrequently employed as a model for the study of themechanisms of stress on ulcer formation.Cold-restraint stress however is not normally展开更多
文摘目的 :观察快动眼睡眠 ( rapid eye movement sleep,REMS)剥夺大鼠行为的改变及其下丘脑诱生型一氧化氮合酶( i NOS) m RNA表达的时相变化。方法 :小站台水环境法剥夺大鼠睡眠 2 4、4 8、72 h后 ,用旷场实验 ( open field test,OFT)观察大鼠主动行为的变化 ,同时用半定量逆转录聚合酶链反应 ( RT- PCR)检测其下丘脑 i NOS m RNA的表达。结果 :睡眠剥夺2 4、4 8h组大鼠的旷场实验得分较非剥夺组高 ( P<0 .0 1) ,剥夺 72 h后降低 ;睡眠剥夺 2 4 h及 4 8h组 i NOS m RNA的表达量无显著变化 ,但剥夺 72 h组 i NOS m RNA的表达量大幅度增加 ( P<0 .0 1)。结论 :内源性一氧化氮 ( NO)增加可能是睡眠剥夺后机体精神行为变化的重要原因 ,高水平的
文摘AIM: To investigate if sleep deprivation is able to increase the expression of inducible heat shock protein 70 in gastric mucosa and its possible role in mucosal defense. METHODS: Rats for sleep disruption were placed inside a computerized rotating drum, gastric mucosa was taken from rats with 1, 3 and 7d sleep deprivation. RT-PCR, immunohistochemistry and Western blotting were used to determine the expression of heat shock protein 70. Ethanol (500mL.L(-1), i.g.) was used to induce gastric mucosa damage. RESULTS: RT-PCR, Western blotting and immunostaining confirmed that the sleep deprivation as a stress resulted in significantly greater expression of inducible heat shock protein 70 in gastric mucosa of rats. After the 500mL.L(-1) ethanol challenge, the ulcer area found in the rats with 7d sleep deprivation (19.15 +/- 4.2)mm(2) was significantly lower (P【0.01) than the corresponding control (53.7 +/- 8.1) mm(2). CONCLUSION: Sleep deprivation as a stress, in addition to lowering the gastric mucosal barrier, is able to stimulate the expression of inducible heat shock protein 70 in gastric mucosa of rats, the heat shock protein 70 may play an important role in gastric mucosal protection.
文摘目的分析ICU患者睡眠障碍的研究文献,了解ICU患者睡眠障碍的发展趋势、研究热点,为ICU患者睡眠障碍相关研究提供借鉴和参考。方法在中国知网数据库(CNKI)和Web of science(WOS)数据库,以MeSH主题词和布尔逻辑运算符对ICU患者睡眠剥夺主题进行检索(时间为建库-2020年),应用可视化分析软件Citespace进行文献计量学分析。结果CNKI检索出398篇,WOS共检索出584篇,发文量总体上呈现增加的趋势,高频关键词为综合护理、集束护理、舒适护理、护理对策等,分析出的突显词为镇静镇痛、彼斯堡睡眠指数及综合护理等。结论ICU患者睡眠剥夺的护理日益受到护理研究人员的关注,ICU患者睡眠剥夺的综合护理的价值开始得到更多的认可。但ICU患者睡眠剥夺的研究测量工具在临床实验和实践中有待进一步的发展,对ICU患者睡眠剥夺的整体护理有待更多的临床实践去发展。
基金supported by the National Natural Science Foundation of China,No.81730033(to XPG),No.81701371(to TJX),No.81801380(to XZ)the Natural Science Foundation of Jiangsu Province of China,No.BK20170654(to TJX),No.BK20170129(to XZ)the Key Talent’s 13th Five-Year Plan for Strengthening Health of Jiangsu Province of China,No.ZDRCA2016069(to XPG)
文摘Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive dysfunction.Photoacoustic treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern experiments.In this study,we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance model.We established a model of sleep rhythm disturbance in C57 BL/6 J mice via a sleep deprivation method involving manual cage tapping,cage jostling,and nest disturbance.We used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive function.Our results indicated that an intervention in which the mice were exposed to blue light(40-Hz flickering frequency)for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep deprivation.However,interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points,as well as 2 Hz-blue light at 3 intervention time points(subjective day time,subjective night time,and offset time points),had no positive effects on circadian rhythm shift or memory deficits.Additionally,a 2000-Hz sound intervention during subjective day time attenuated the24-hour-acrophase shift and memory decline,while 440-Hz and 4000-Hz sounds had no effect on circadian rhythms.Overall,these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake rhythm.All animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University,China(approval No.20171102)on November20,2017.
文摘Sleep hygiene and knowledge about sleep basics is unknown in college student populations. The college student population have abbreviated sleep due to social schedules and busy academic schedules. A measurement of sleep hygiene and knowledge about sleep in the college student population indicates that there are varying levels along with sleep knowledge. The objective of the study was to examine young adult sleep quality because of providing an educative intervention. A sleep class of one hour provided once a week for ten weeks was provided following the administration of a ten-item sleep survey. Following the sleep class, the sleep survey was read ministered. Measures of central tendency and non-parametric analysis of nominal data were conducted. The results indicated two of the five cohorts had statistically significant differences. Further, the measures of sleep quality differed, statistically, for two of the cohorts receiving the classroom instruction. This research finding indicates a continued need to educate college student populations about their sleep health.
基金the CRCG grant from the University of Hong Kong.
文摘INTRODUCTIONStress has been shown to induce gastric mucosallesions and lower the effectiveness of the mucosa asa barrier.In rats,gastric ulcers can beproduced by cold-restraint stress and it isfrequently employed as a model for the study of themechanisms of stress on ulcer formation.Cold-restraint stress however is not normally
