Ⅰ. INTRODUCTIONThe chelates of chiral chelating resins with ions of transition metals can be used as staionary phases for ligand-exchange chromatography on which some racemates of amino acids may be resolved. During ...Ⅰ. INTRODUCTIONThe chelates of chiral chelating resins with ions of transition metals can be used as staionary phases for ligand-exchange chromatography on which some racemates of amino acids may be resolved. During the chromatographic process, the mass transfer of an amino acid is affected significantly by the polarity of the supports used in the chiral chelating resins.展开更多
L-proline was linked onto cross-linked polystyrene to give chiral chelating resin (1). The copper (Ⅱ) chelate of resin (1) was used as the chiral stationary phase for the chromatographic resolution of DL-amino acids....L-proline was linked onto cross-linked polystyrene to give chiral chelating resin (1). The copper (Ⅱ) chelate of resin (1) was used as the chiral stationary phase for the chromatographic resolution of DL-amino acids. However, the hydrophobicity of polystyrene causes an amino acid to transfer slowly during chromatographic process so展开更多
利用实验室筛选得到的高立体选择性脂肪酶产生菌株溜曲霉(Aspergillus tamarii WYC3)不对称水解拆分N-BOC-DL-α-氨基丁酸甲酯得到N-BOC-L-α-氨基丁酸,对其反应条件进行研究,确定其最优反应条件为:0.1 mol/L p H 7.2的磷酸钾缓冲液,反...利用实验室筛选得到的高立体选择性脂肪酶产生菌株溜曲霉(Aspergillus tamarii WYC3)不对称水解拆分N-BOC-DL-α-氨基丁酸甲酯得到N-BOC-L-α-氨基丁酸,对其反应条件进行研究,确定其最优反应条件为:0.1 mol/L p H 7.2的磷酸钾缓冲液,反应温度30℃,底物浓度52.24 mmol/L,0.1 g菌粉,V(底物)∶V(丙酮)=1∶8,200 r/min恒温水浴反应6 h,可获得产物N-BOC-L-α-氨基丁酸,此时e.e._(s)值达到99.95%,对映体选择率E值为20.11,产率为36.21%。展开更多
文摘Ⅰ. INTRODUCTIONThe chelates of chiral chelating resins with ions of transition metals can be used as staionary phases for ligand-exchange chromatography on which some racemates of amino acids may be resolved. During the chromatographic process, the mass transfer of an amino acid is affected significantly by the polarity of the supports used in the chiral chelating resins.
文摘L-proline was linked onto cross-linked polystyrene to give chiral chelating resin (1). The copper (Ⅱ) chelate of resin (1) was used as the chiral stationary phase for the chromatographic resolution of DL-amino acids. However, the hydrophobicity of polystyrene causes an amino acid to transfer slowly during chromatographic process so
