采用变性梯度凝胶电泳（DGGE）技术对内蒙古河套灌区三种不同盐碱程度土壤（盐土,强度盐化土,轻度盐化土）不同深度（0~20cm和20~30cm）土壤细菌16S r DNA V3~V6可变区扩增片段进行分析,并对土壤理化性质进行了测定.结果表明：细菌群落...采用变性梯度凝胶电泳（DGGE）技术对内蒙古河套灌区三种不同盐碱程度土壤（盐土,强度盐化土,轻度盐化土）不同深度（0~20cm和20~30cm）土壤细菌16S r DNA V3~V6可变区扩增片段进行分析,并对土壤理化性质进行了测定.结果表明：细菌群落多样性随土壤盐碱化程度的加深而减少（轻度盐化土〉强度盐化土〉盐土）,随土壤深度的增加而降低（细菌群落多样性0~20cm土层大于20~30cm土层）.细菌Shannon-Wiener指数在轻度盐化土中最大为3.36,在强度盐化土和盐土分别为3.05和2.49.不同盐碱程度土壤以细菌相似系数聚类,分为0~20cm层与20~30cm层两大族群,土壤细菌群落Shannon-Wiener指数在0~20cm层中（盐土为3.04,强度盐化土为3.29,轻度盐化土为3.36）均大于在20~30cm层（盐土为2.49,强度盐化土为3.05,轻度盐化土为3.14）.相关性分析和典范对应分析表明,土壤w（EC）、p H值、w（SOC）、w（TP）是土壤细菌群落结构多样性的显著影响因素,不同盐碱程度土壤中细菌群落的Shannon-Wiener指数与土壤w（EC）（r=-0.542,P〈0.05）、p H（r=-0.526,P〈0.05）呈显著负相关,与土壤w（SOC）（r=0.700,P〈0.01）和w（TP）（r=0.805,P〈0.01）呈极显著正相关.w（EC）和p H对盐碱土壤细菌群落结构的影响力最大.回收DGGE图谱中20个优势条带进行测序分析,结果显示,变形菌纲（α-变形菌纲、β-变形菌纲、γ-变形菌纲和δ-变形菌纲）是盐碱土壤的主要类群.展开更多
Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis...Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels （50, 150, and 250 mg/kg） were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos （250 mg/kg） could significantly stimulate fungal populations. DGGE （denaturing gradient gel electrophoresis） fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.展开更多
浸矿体系中的菌群结构及其演替规律与浸出率具有密切的关系,为了解硫化镍矿浸矿体系中的这种关系以提高浸出率,利用PCR-DGGE(变性梯度凝胶电泳)技术结合16 S rRNA基因序列对体系的细菌种群结构进行分析.研究表明,在硫化镍矿浸矿体系中,...浸矿体系中的菌群结构及其演替规律与浸出率具有密切的关系,为了解硫化镍矿浸矿体系中的这种关系以提高浸出率,利用PCR-DGGE(变性梯度凝胶电泳)技术结合16 S rRNA基因序列对体系的细菌种群结构进行分析.研究表明,在硫化镍矿浸矿体系中,菌群组成菌属为嗜酸氧化亚铁硫杆菌属(Acidithiobacillus),并在浸矿体系中具有明显的演替现象,浸矿体系中的菌株演替和浸出率具有明显的相关性,经过21d的浸出,硫化镍矿的浸出率达到70%,探索浸矿体系中细菌的种群结构及其演替与浸出率的关系,对优化浸出体系菌群组成,提高浸出率具有指导作用.展开更多
文摘采用变性梯度凝胶电泳（DGGE）技术对内蒙古河套灌区三种不同盐碱程度土壤（盐土,强度盐化土,轻度盐化土）不同深度（0~20cm和20~30cm）土壤细菌16S r DNA V3~V6可变区扩增片段进行分析,并对土壤理化性质进行了测定.结果表明：细菌群落多样性随土壤盐碱化程度的加深而减少（轻度盐化土〉强度盐化土〉盐土）,随土壤深度的增加而降低（细菌群落多样性0~20cm土层大于20~30cm土层）.细菌Shannon-Wiener指数在轻度盐化土中最大为3.36,在强度盐化土和盐土分别为3.05和2.49.不同盐碱程度土壤以细菌相似系数聚类,分为0~20cm层与20~30cm层两大族群,土壤细菌群落Shannon-Wiener指数在0~20cm层中（盐土为3.04,强度盐化土为3.29,轻度盐化土为3.36）均大于在20~30cm层（盐土为2.49,强度盐化土为3.05,轻度盐化土为3.14）.相关性分析和典范对应分析表明,土壤w（EC）、p H值、w（SOC）、w（TP）是土壤细菌群落结构多样性的显著影响因素,不同盐碱程度土壤中细菌群落的Shannon-Wiener指数与土壤w（EC）（r=-0.542,P〈0.05）、p H（r=-0.526,P〈0.05）呈显著负相关,与土壤w（SOC）（r=0.700,P〈0.01）和w（TP）（r=0.805,P〈0.01）呈极显著正相关.w（EC）和p H对盐碱土壤细菌群落结构的影响力最大.回收DGGE图谱中20个优势条带进行测序分析,结果显示,变形菌纲（α-变形菌纲、β-变形菌纲、γ-变形菌纲和δ-变形菌纲）是盐碱土壤的主要类群.
文摘Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels （50, 150, and 250 mg/kg） were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos （250 mg/kg） could significantly stimulate fungal populations. DGGE （denaturing gradient gel electrophoresis） fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.
文摘浸矿体系中的菌群结构及其演替规律与浸出率具有密切的关系,为了解硫化镍矿浸矿体系中的这种关系以提高浸出率,利用PCR-DGGE(变性梯度凝胶电泳)技术结合16 S rRNA基因序列对体系的细菌种群结构进行分析.研究表明,在硫化镍矿浸矿体系中,菌群组成菌属为嗜酸氧化亚铁硫杆菌属(Acidithiobacillus),并在浸矿体系中具有明显的演替现象,浸矿体系中的菌株演替和浸出率具有明显的相关性,经过21d的浸出,硫化镍矿的浸出率达到70%,探索浸矿体系中细菌的种群结构及其演替与浸出率的关系,对优化浸出体系菌群组成,提高浸出率具有指导作用.
